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Magnetic Levitation of Personalized Nanoparticle–Protein Corona as an Effective Tool for Cancer Detection

Unprecedented opportunities for early stage cancer detection have recently emerged from the characterization of the personalized protein corona (PC), i.e., the protein cloud that surrounds nanoparticles (NPs) upon exposure to a patients’ bodily fluids. Most of these methods require “direct character...

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Autores principales: Quagliarini, Erica, Digiacomo, Luca, Caputo, Damiano, Coppola, Alessandro, Amenitsch, Heinz, Caracciolo, Giulio, Pozzi, Daniela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104194/
https://www.ncbi.nlm.nih.gov/pubmed/35564106
http://dx.doi.org/10.3390/nano12091397
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author Quagliarini, Erica
Digiacomo, Luca
Caputo, Damiano
Coppola, Alessandro
Amenitsch, Heinz
Caracciolo, Giulio
Pozzi, Daniela
author_facet Quagliarini, Erica
Digiacomo, Luca
Caputo, Damiano
Coppola, Alessandro
Amenitsch, Heinz
Caracciolo, Giulio
Pozzi, Daniela
author_sort Quagliarini, Erica
collection PubMed
description Unprecedented opportunities for early stage cancer detection have recently emerged from the characterization of the personalized protein corona (PC), i.e., the protein cloud that surrounds nanoparticles (NPs) upon exposure to a patients’ bodily fluids. Most of these methods require “direct characterization” of the PC., i.e., they necessitate protein isolation, identification, and quantification. Each of these steps can introduce bias and affect reproducibility and inter-laboratory consistency of experimental data. To fulfill this gap, here we develop a nanoparticle-enabled blood (NEB) test based on the indirect characterization of the personalized PC by magnetic levitation (MagLev). The MagLev NEB test works by analyzing the levitation profiles of PC-coated graphene oxide (GO) NPs that migrate along a magnetic field gradient in a paramagnetic medium. For the test validation, we employed human plasma samples from 15 healthy individuals and 30 oncological patients affected by four cancer types, namely breast cancer, prostate cancer, colorectal cancer, and pancreatic ductal adenocarcinoma (PDAC). Over the last 15 years prostate cancer, colorectal cancer, and PDAC have continuously been the second, third, and fourth leading sites of cancer-related deaths in men, while breast cancer, colorectal cancer, and PDAC are the second, third and fourth leading sites for women. This proof-of-concept investigation shows that the sensitivity and specificity of the MagLev NEB test depend on the cancer type, with the global classification accuracy ranging from 70% for prostate cancer to an impressive 93.3% for PDAC. We also discuss how this tool could benefit from several tunable parameters (e.g., the intensity of magnetic field gradient, NP type, exposure conditions, etc.) that can be modulated to optimize the detection of different cancer types with high sensitivity and specificity.
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spelling pubmed-91041942022-05-14 Magnetic Levitation of Personalized Nanoparticle–Protein Corona as an Effective Tool for Cancer Detection Quagliarini, Erica Digiacomo, Luca Caputo, Damiano Coppola, Alessandro Amenitsch, Heinz Caracciolo, Giulio Pozzi, Daniela Nanomaterials (Basel) Communication Unprecedented opportunities for early stage cancer detection have recently emerged from the characterization of the personalized protein corona (PC), i.e., the protein cloud that surrounds nanoparticles (NPs) upon exposure to a patients’ bodily fluids. Most of these methods require “direct characterization” of the PC., i.e., they necessitate protein isolation, identification, and quantification. Each of these steps can introduce bias and affect reproducibility and inter-laboratory consistency of experimental data. To fulfill this gap, here we develop a nanoparticle-enabled blood (NEB) test based on the indirect characterization of the personalized PC by magnetic levitation (MagLev). The MagLev NEB test works by analyzing the levitation profiles of PC-coated graphene oxide (GO) NPs that migrate along a magnetic field gradient in a paramagnetic medium. For the test validation, we employed human plasma samples from 15 healthy individuals and 30 oncological patients affected by four cancer types, namely breast cancer, prostate cancer, colorectal cancer, and pancreatic ductal adenocarcinoma (PDAC). Over the last 15 years prostate cancer, colorectal cancer, and PDAC have continuously been the second, third, and fourth leading sites of cancer-related deaths in men, while breast cancer, colorectal cancer, and PDAC are the second, third and fourth leading sites for women. This proof-of-concept investigation shows that the sensitivity and specificity of the MagLev NEB test depend on the cancer type, with the global classification accuracy ranging from 70% for prostate cancer to an impressive 93.3% for PDAC. We also discuss how this tool could benefit from several tunable parameters (e.g., the intensity of magnetic field gradient, NP type, exposure conditions, etc.) that can be modulated to optimize the detection of different cancer types with high sensitivity and specificity. MDPI 2022-04-19 /pmc/articles/PMC9104194/ /pubmed/35564106 http://dx.doi.org/10.3390/nano12091397 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Quagliarini, Erica
Digiacomo, Luca
Caputo, Damiano
Coppola, Alessandro
Amenitsch, Heinz
Caracciolo, Giulio
Pozzi, Daniela
Magnetic Levitation of Personalized Nanoparticle–Protein Corona as an Effective Tool for Cancer Detection
title Magnetic Levitation of Personalized Nanoparticle–Protein Corona as an Effective Tool for Cancer Detection
title_full Magnetic Levitation of Personalized Nanoparticle–Protein Corona as an Effective Tool for Cancer Detection
title_fullStr Magnetic Levitation of Personalized Nanoparticle–Protein Corona as an Effective Tool for Cancer Detection
title_full_unstemmed Magnetic Levitation of Personalized Nanoparticle–Protein Corona as an Effective Tool for Cancer Detection
title_short Magnetic Levitation of Personalized Nanoparticle–Protein Corona as an Effective Tool for Cancer Detection
title_sort magnetic levitation of personalized nanoparticle–protein corona as an effective tool for cancer detection
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104194/
https://www.ncbi.nlm.nih.gov/pubmed/35564106
http://dx.doi.org/10.3390/nano12091397
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