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Development of Microscopic Techniques for the Visualization of Plant–Root-Knot Nematode Interaction
Plant-parasitic nematodes are a significant cause of yield losses and food security issues. Specifically, nematodes of the genus Meloidogyne can cause significant production losses in horticultural crops around the world. Understanding the mechanisms of the ever-changing physiology of plant roots by...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104198/ https://www.ncbi.nlm.nih.gov/pubmed/35567165 http://dx.doi.org/10.3390/plants11091165 |
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author | Vernet, Helena Fullana, Aïda Magdalena Sorribas, Francisco Javier Gualda, Emilio J. |
author_facet | Vernet, Helena Fullana, Aïda Magdalena Sorribas, Francisco Javier Gualda, Emilio J. |
author_sort | Vernet, Helena |
collection | PubMed |
description | Plant-parasitic nematodes are a significant cause of yield losses and food security issues. Specifically, nematodes of the genus Meloidogyne can cause significant production losses in horticultural crops around the world. Understanding the mechanisms of the ever-changing physiology of plant roots by imaging the galls induced by nematodes could provide a great insight into their control. However, infected roots are unsuitable for light microscopy investigation due to the opacity of plant tissues. Thus, samples must be cleared to visualize the interior of whole plants in order to make them transparent using clearing agents. This work aims to identify which clearing protocol and microscopy system is the most appropriate to obtain 3D images of tomato cv. Durinta and eggplant cv. Cristal samples infected with Meloidogyne incognita to visualize and study the root–nematode interaction. To that extent, two clearing solutions (BABB and ECi), combined with three different dehydration solvents (ethanol, methanol and 1-propanol), are tested. In addition, the advantages and disadvantages of alternative imaging techniques to confocal microscopy are analyzed by employing an experimental custom-made setup that combines two microscopic techniques, light sheet fluorescence microscopy and optical projection tomography, on a single instrument. |
format | Online Article Text |
id | pubmed-9104198 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91041982022-05-14 Development of Microscopic Techniques for the Visualization of Plant–Root-Knot Nematode Interaction Vernet, Helena Fullana, Aïda Magdalena Sorribas, Francisco Javier Gualda, Emilio J. Plants (Basel) Article Plant-parasitic nematodes are a significant cause of yield losses and food security issues. Specifically, nematodes of the genus Meloidogyne can cause significant production losses in horticultural crops around the world. Understanding the mechanisms of the ever-changing physiology of plant roots by imaging the galls induced by nematodes could provide a great insight into their control. However, infected roots are unsuitable for light microscopy investigation due to the opacity of plant tissues. Thus, samples must be cleared to visualize the interior of whole plants in order to make them transparent using clearing agents. This work aims to identify which clearing protocol and microscopy system is the most appropriate to obtain 3D images of tomato cv. Durinta and eggplant cv. Cristal samples infected with Meloidogyne incognita to visualize and study the root–nematode interaction. To that extent, two clearing solutions (BABB and ECi), combined with three different dehydration solvents (ethanol, methanol and 1-propanol), are tested. In addition, the advantages and disadvantages of alternative imaging techniques to confocal microscopy are analyzed by employing an experimental custom-made setup that combines two microscopic techniques, light sheet fluorescence microscopy and optical projection tomography, on a single instrument. MDPI 2022-04-26 /pmc/articles/PMC9104198/ /pubmed/35567165 http://dx.doi.org/10.3390/plants11091165 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Vernet, Helena Fullana, Aïda Magdalena Sorribas, Francisco Javier Gualda, Emilio J. Development of Microscopic Techniques for the Visualization of Plant–Root-Knot Nematode Interaction |
title | Development of Microscopic Techniques for the Visualization of Plant–Root-Knot Nematode Interaction |
title_full | Development of Microscopic Techniques for the Visualization of Plant–Root-Knot Nematode Interaction |
title_fullStr | Development of Microscopic Techniques for the Visualization of Plant–Root-Knot Nematode Interaction |
title_full_unstemmed | Development of Microscopic Techniques for the Visualization of Plant–Root-Knot Nematode Interaction |
title_short | Development of Microscopic Techniques for the Visualization of Plant–Root-Knot Nematode Interaction |
title_sort | development of microscopic techniques for the visualization of plant–root-knot nematode interaction |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104198/ https://www.ncbi.nlm.nih.gov/pubmed/35567165 http://dx.doi.org/10.3390/plants11091165 |
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