The Anti-Glucocorticoid Receptor Antibody Clone 5E4: Raising Awareness of Unspecific Antibody Binding

Unspecific antibody binding takes a significant toll on researchers in the form of both the economic burden and the disappointed hopes of promising new therapeutic targets. Despite recent initiatives promoting antibody validation, a uniform approach addressing this issue has not yet been developed....

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Autores principales: Ehlers, Lisa, Kirchner, Marieluise, Mertins, Philipp, Strehl, Cindy, Buttgereit, Frank, Gaber, Timo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104323/
https://www.ncbi.nlm.nih.gov/pubmed/35563440
http://dx.doi.org/10.3390/ijms23095049
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author Ehlers, Lisa
Kirchner, Marieluise
Mertins, Philipp
Strehl, Cindy
Buttgereit, Frank
Gaber, Timo
author_facet Ehlers, Lisa
Kirchner, Marieluise
Mertins, Philipp
Strehl, Cindy
Buttgereit, Frank
Gaber, Timo
author_sort Ehlers, Lisa
collection PubMed
description Unspecific antibody binding takes a significant toll on researchers in the form of both the economic burden and the disappointed hopes of promising new therapeutic targets. Despite recent initiatives promoting antibody validation, a uniform approach addressing this issue has not yet been developed. Here, we demonstrate that the anti-glucocorticoid receptor (GR) antibody clone 5E4 predominantly targets two different proteins of approximately the same size, namely AMP deaminase 2 (AMPD2) and transcription intermediary factor 1-beta (TRIM28). This paper is intended to generate awareness of unspecific binding of well-established reagents and advocate the use of more rigorous verification methods to improve antibody quality in the future.
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spelling pubmed-91043232022-05-14 The Anti-Glucocorticoid Receptor Antibody Clone 5E4: Raising Awareness of Unspecific Antibody Binding Ehlers, Lisa Kirchner, Marieluise Mertins, Philipp Strehl, Cindy Buttgereit, Frank Gaber, Timo Int J Mol Sci Article Unspecific antibody binding takes a significant toll on researchers in the form of both the economic burden and the disappointed hopes of promising new therapeutic targets. Despite recent initiatives promoting antibody validation, a uniform approach addressing this issue has not yet been developed. Here, we demonstrate that the anti-glucocorticoid receptor (GR) antibody clone 5E4 predominantly targets two different proteins of approximately the same size, namely AMP deaminase 2 (AMPD2) and transcription intermediary factor 1-beta (TRIM28). This paper is intended to generate awareness of unspecific binding of well-established reagents and advocate the use of more rigorous verification methods to improve antibody quality in the future. MDPI 2022-05-02 /pmc/articles/PMC9104323/ /pubmed/35563440 http://dx.doi.org/10.3390/ijms23095049 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ehlers, Lisa
Kirchner, Marieluise
Mertins, Philipp
Strehl, Cindy
Buttgereit, Frank
Gaber, Timo
The Anti-Glucocorticoid Receptor Antibody Clone 5E4: Raising Awareness of Unspecific Antibody Binding
title The Anti-Glucocorticoid Receptor Antibody Clone 5E4: Raising Awareness of Unspecific Antibody Binding
title_full The Anti-Glucocorticoid Receptor Antibody Clone 5E4: Raising Awareness of Unspecific Antibody Binding
title_fullStr The Anti-Glucocorticoid Receptor Antibody Clone 5E4: Raising Awareness of Unspecific Antibody Binding
title_full_unstemmed The Anti-Glucocorticoid Receptor Antibody Clone 5E4: Raising Awareness of Unspecific Antibody Binding
title_short The Anti-Glucocorticoid Receptor Antibody Clone 5E4: Raising Awareness of Unspecific Antibody Binding
title_sort anti-glucocorticoid receptor antibody clone 5e4: raising awareness of unspecific antibody binding
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104323/
https://www.ncbi.nlm.nih.gov/pubmed/35563440
http://dx.doi.org/10.3390/ijms23095049
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