Cargando…

Lymph Nodes Draining Infections Investigated by PET and Immunohistochemistry in a Juvenile Porcine Model

Background: [(18)F]FDG and [(11)C]methionine accumulate in lymph nodes draining S. aureus -infected foci. The lymph nodes were characterized by weight, [(11)C]methionine- and [(18)F]FDG-positron emissions tomography (PET)/computed tomography (CT), and immunohistochemical (IHC)-staining. Methods: 20...

Descripción completa

Detalles Bibliográficos
Autores principales: Afzelius, Pia, Morsing, Malene Kjelin, Nielsen, Ole Lerberg, Alstrup, Aage Kristian Olsen, Jensen, Svend Borup, Jødal, Lars
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104488/
https://www.ncbi.nlm.nih.gov/pubmed/35566137
http://dx.doi.org/10.3390/molecules27092792
Descripción
Sumario:Background: [(18)F]FDG and [(11)C]methionine accumulate in lymph nodes draining S. aureus -infected foci. The lymph nodes were characterized by weight, [(11)C]methionine- and [(18)F]FDG-positron emissions tomography (PET)/computed tomography (CT), and immunohistochemical (IHC)-staining. Methods: 20 pigs inoculated with S. aureus into the right femoral artery were PET/CT-scanned with [(18)F]FDG, and nine of the pigs were additionally scanned with [(11)C]methionine. Mammary, medial iliac, and popliteal lymph nodes from the left and right hind limbs were weighed. IHC-staining for calculations of area fractions of Ki-67, L1, and IL-8 positive cells was done in mammary and popliteal lymph nodes from the nine pigs. Results: The pigs developed one to six osteomyelitis foci. Some pigs developed contiguous infections of peri-osseous tissue and inoculation-site abscesses. Weights of mammary and medial iliac lymph nodes and their [(18)F]FDG maximum Standardized Uptake Values (SUV(FDGmax)) showed a significant increase in the inoculated limb compared to the left limb. Popliteal lymph node weight and their FDG uptake did not differ significantly between hind limbs. Area fractions of Ki-67 and IL-8 in the right mammary lymph nodes and SUV(Metmax) in the right popliteal lymph nodes were significantly increased compared with the left side. Conclusion: The PET-tracers [(18)F]FDG and [(11)C]methionine, and the IHC- markers Ki-67 and IL-8, but not L1, showed increased values in lymph nodes draining soft tissues infected with S. aureus. The increase in [(11)C]methionine may indicate a more acute lymph node response, whereas an increase in [(18)F]FDG may indicate a more chronic response.