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Genome-Wide Investigation of G6PDH Gene in Strawberry: Evolution and Expression Analysis during Development and Stress

As one of the key enzymes in the pentose phosphate pathway (PPP), glucose-6-phosphate dehydrogenase (G6PDH) provides NADPH and plays an important role in plant development and stress responses. However, little information was available about the G6PDH genes in strawberry (Fragaria × ananassa). The r...

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Autores principales: Lei, Diya, Lin, Yuanxiu, Luo, Mengwen, Zhao, Bing, Tang, Honglan, Zhou, Xuan, Yao, Wantian, Zhang, Yunting, Wang, Yan, Li, Mengyao, Chen, Qing, Luo, Ya, Wang, Xiaorong, Tang, Haoru, Zhang, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104510/
https://www.ncbi.nlm.nih.gov/pubmed/35563120
http://dx.doi.org/10.3390/ijms23094728
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author Lei, Diya
Lin, Yuanxiu
Luo, Mengwen
Zhao, Bing
Tang, Honglan
Zhou, Xuan
Yao, Wantian
Zhang, Yunting
Wang, Yan
Li, Mengyao
Chen, Qing
Luo, Ya
Wang, Xiaorong
Tang, Haoru
Zhang, Yong
author_facet Lei, Diya
Lin, Yuanxiu
Luo, Mengwen
Zhao, Bing
Tang, Honglan
Zhou, Xuan
Yao, Wantian
Zhang, Yunting
Wang, Yan
Li, Mengyao
Chen, Qing
Luo, Ya
Wang, Xiaorong
Tang, Haoru
Zhang, Yong
author_sort Lei, Diya
collection PubMed
description As one of the key enzymes in the pentose phosphate pathway (PPP), glucose-6-phosphate dehydrogenase (G6PDH) provides NADPH and plays an important role in plant development and stress responses. However, little information was available about the G6PDH genes in strawberry (Fragaria × ananassa). The recent release of the whole-genome sequence of strawberry allowed us to perform a genome-wide investigation into the organization and expression profiling of strawberry G6PDH genes. In the present study, 19 strawberry G6PDH genes (FaG6PDHs) were identified from the strawberry genome database. They were designated as FaG6PDH1 to FaG6PDH19, respectively, according to the conserved domain of each subfamily and multiple sequence alignment with Arabidopsis. According to their structural and phylogenetic features, the 19 FaG6PDHs were further classified into five types: Cy, P1, P1.1, P2 and PO. The number and location of exons and introns are similar, suggesting that genes of the same type are very similar and are alleles. A cis-element analysis inferred that FaG6PDHs possessed at least one stress-responsive cis-acting element. Expression profiles derived from transcriptome data analysis exhibited distinct expression patterns of FaG6PDHs genes in different developmental stages. Real-time quantitative PCR was used to detect the expression level of five types FaG6PDHs genes and demonstrated that the genes were expressed and responded to multiple abiotic stress and hormonal treatments.
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spelling pubmed-91045102022-05-14 Genome-Wide Investigation of G6PDH Gene in Strawberry: Evolution and Expression Analysis during Development and Stress Lei, Diya Lin, Yuanxiu Luo, Mengwen Zhao, Bing Tang, Honglan Zhou, Xuan Yao, Wantian Zhang, Yunting Wang, Yan Li, Mengyao Chen, Qing Luo, Ya Wang, Xiaorong Tang, Haoru Zhang, Yong Int J Mol Sci Article As one of the key enzymes in the pentose phosphate pathway (PPP), glucose-6-phosphate dehydrogenase (G6PDH) provides NADPH and plays an important role in plant development and stress responses. However, little information was available about the G6PDH genes in strawberry (Fragaria × ananassa). The recent release of the whole-genome sequence of strawberry allowed us to perform a genome-wide investigation into the organization and expression profiling of strawberry G6PDH genes. In the present study, 19 strawberry G6PDH genes (FaG6PDHs) were identified from the strawberry genome database. They were designated as FaG6PDH1 to FaG6PDH19, respectively, according to the conserved domain of each subfamily and multiple sequence alignment with Arabidopsis. According to their structural and phylogenetic features, the 19 FaG6PDHs were further classified into five types: Cy, P1, P1.1, P2 and PO. The number and location of exons and introns are similar, suggesting that genes of the same type are very similar and are alleles. A cis-element analysis inferred that FaG6PDHs possessed at least one stress-responsive cis-acting element. Expression profiles derived from transcriptome data analysis exhibited distinct expression patterns of FaG6PDHs genes in different developmental stages. Real-time quantitative PCR was used to detect the expression level of five types FaG6PDHs genes and demonstrated that the genes were expressed and responded to multiple abiotic stress and hormonal treatments. MDPI 2022-04-25 /pmc/articles/PMC9104510/ /pubmed/35563120 http://dx.doi.org/10.3390/ijms23094728 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lei, Diya
Lin, Yuanxiu
Luo, Mengwen
Zhao, Bing
Tang, Honglan
Zhou, Xuan
Yao, Wantian
Zhang, Yunting
Wang, Yan
Li, Mengyao
Chen, Qing
Luo, Ya
Wang, Xiaorong
Tang, Haoru
Zhang, Yong
Genome-Wide Investigation of G6PDH Gene in Strawberry: Evolution and Expression Analysis during Development and Stress
title Genome-Wide Investigation of G6PDH Gene in Strawberry: Evolution and Expression Analysis during Development and Stress
title_full Genome-Wide Investigation of G6PDH Gene in Strawberry: Evolution and Expression Analysis during Development and Stress
title_fullStr Genome-Wide Investigation of G6PDH Gene in Strawberry: Evolution and Expression Analysis during Development and Stress
title_full_unstemmed Genome-Wide Investigation of G6PDH Gene in Strawberry: Evolution and Expression Analysis during Development and Stress
title_short Genome-Wide Investigation of G6PDH Gene in Strawberry: Evolution and Expression Analysis during Development and Stress
title_sort genome-wide investigation of g6pdh gene in strawberry: evolution and expression analysis during development and stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104510/
https://www.ncbi.nlm.nih.gov/pubmed/35563120
http://dx.doi.org/10.3390/ijms23094728
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