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Binary Effects of Gynostemma Gold Nanoparticles on Obesity and Inflammation via Downregulation of PPARγ/CEPBα and TNF-α Gene Expression

Nanoscience is a multidisciplinary skill with elucidated nanoscale particles and their advantages in applications to various fields. Owing to their economical synthesis, biocompatible nature, and widespread biomedical and environmental applications, the green synthesis of metal nanoparticles using m...

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Autores principales: Akter, Reshmi, Ling, Li, Rupa, Esrat Jahan, KyuPark, Jin, Mathiyalagan, Ramya, Nahar, Jinnatun, Won, Lee Jong, Hyun, Kim Do, Murugesan, Mohanapriya, Yang, Deok Chun, Kang, Se Chan, Kwak, Gi-Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104634/
https://www.ncbi.nlm.nih.gov/pubmed/35566145
http://dx.doi.org/10.3390/molecules27092795
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author Akter, Reshmi
Ling, Li
Rupa, Esrat Jahan
KyuPark, Jin
Mathiyalagan, Ramya
Nahar, Jinnatun
Won, Lee Jong
Hyun, Kim Do
Murugesan, Mohanapriya
Yang, Deok Chun
Kang, Se Chan
Kwak, Gi-Young
author_facet Akter, Reshmi
Ling, Li
Rupa, Esrat Jahan
KyuPark, Jin
Mathiyalagan, Ramya
Nahar, Jinnatun
Won, Lee Jong
Hyun, Kim Do
Murugesan, Mohanapriya
Yang, Deok Chun
Kang, Se Chan
Kwak, Gi-Young
author_sort Akter, Reshmi
collection PubMed
description Nanoscience is a multidisciplinary skill with elucidated nanoscale particles and their advantages in applications to various fields. Owing to their economical synthesis, biocompatible nature, and widespread biomedical and environmental applications, the green synthesis of metal nanoparticles using medicinal plants has become a potential research area in biomedical research and functional food formulations. Gynostemma pentaphyllum (GP) has been extensively used in traditional Chinese medicine to cure several diseases, including diabetes mellitus (DM). This is the first study in which we examined the efficacy of G. pentaphyllum gold nanoparticles (GP-AuNPs) against obesity and related inflammation. GP extract was used as a capping agent to reduce Au(2+) to Au(0) to form stable gold nanoparticles. The nanoparticles were characterized by using UV–VIS spectroscopy, and TEM images were used to analyze morphology. In contrast, the existence of the functional group was measured using FTIR, and size and shape were examined using XRD analysis. In vitro analysis on GP-AuNPs was nontoxic to RAW 264.7 cells and 3T3-L1 cells up to a specific concentration. It significantly decreased lipid accumulation in 3T3-L1 obese and reduced NO production in Raw 264.7 macrophage cells. The significant adipogenic genes PPARγ and CEPBα and a major pro-inflammatory cytokine TNF-α expression were quantified using RT-PCR. The GP-AuNPs decreased the face of these genes remarkably, revealing the antiadipogenic and anti-inflammatory activity of our synthesized GP-AuNPs. This study represents thorough research on the antiobesity effect of Gynostemma pentaphyllum gold nanoparticles synthesized using a green approach and the efficacy instead of related inflammatory responses.
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spelling pubmed-91046342022-05-14 Binary Effects of Gynostemma Gold Nanoparticles on Obesity and Inflammation via Downregulation of PPARγ/CEPBα and TNF-α Gene Expression Akter, Reshmi Ling, Li Rupa, Esrat Jahan KyuPark, Jin Mathiyalagan, Ramya Nahar, Jinnatun Won, Lee Jong Hyun, Kim Do Murugesan, Mohanapriya Yang, Deok Chun Kang, Se Chan Kwak, Gi-Young Molecules Article Nanoscience is a multidisciplinary skill with elucidated nanoscale particles and their advantages in applications to various fields. Owing to their economical synthesis, biocompatible nature, and widespread biomedical and environmental applications, the green synthesis of metal nanoparticles using medicinal plants has become a potential research area in biomedical research and functional food formulations. Gynostemma pentaphyllum (GP) has been extensively used in traditional Chinese medicine to cure several diseases, including diabetes mellitus (DM). This is the first study in which we examined the efficacy of G. pentaphyllum gold nanoparticles (GP-AuNPs) against obesity and related inflammation. GP extract was used as a capping agent to reduce Au(2+) to Au(0) to form stable gold nanoparticles. The nanoparticles were characterized by using UV–VIS spectroscopy, and TEM images were used to analyze morphology. In contrast, the existence of the functional group was measured using FTIR, and size and shape were examined using XRD analysis. In vitro analysis on GP-AuNPs was nontoxic to RAW 264.7 cells and 3T3-L1 cells up to a specific concentration. It significantly decreased lipid accumulation in 3T3-L1 obese and reduced NO production in Raw 264.7 macrophage cells. The significant adipogenic genes PPARγ and CEPBα and a major pro-inflammatory cytokine TNF-α expression were quantified using RT-PCR. The GP-AuNPs decreased the face of these genes remarkably, revealing the antiadipogenic and anti-inflammatory activity of our synthesized GP-AuNPs. This study represents thorough research on the antiobesity effect of Gynostemma pentaphyllum gold nanoparticles synthesized using a green approach and the efficacy instead of related inflammatory responses. MDPI 2022-04-27 /pmc/articles/PMC9104634/ /pubmed/35566145 http://dx.doi.org/10.3390/molecules27092795 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Akter, Reshmi
Ling, Li
Rupa, Esrat Jahan
KyuPark, Jin
Mathiyalagan, Ramya
Nahar, Jinnatun
Won, Lee Jong
Hyun, Kim Do
Murugesan, Mohanapriya
Yang, Deok Chun
Kang, Se Chan
Kwak, Gi-Young
Binary Effects of Gynostemma Gold Nanoparticles on Obesity and Inflammation via Downregulation of PPARγ/CEPBα and TNF-α Gene Expression
title Binary Effects of Gynostemma Gold Nanoparticles on Obesity and Inflammation via Downregulation of PPARγ/CEPBα and TNF-α Gene Expression
title_full Binary Effects of Gynostemma Gold Nanoparticles on Obesity and Inflammation via Downregulation of PPARγ/CEPBα and TNF-α Gene Expression
title_fullStr Binary Effects of Gynostemma Gold Nanoparticles on Obesity and Inflammation via Downregulation of PPARγ/CEPBα and TNF-α Gene Expression
title_full_unstemmed Binary Effects of Gynostemma Gold Nanoparticles on Obesity and Inflammation via Downregulation of PPARγ/CEPBα and TNF-α Gene Expression
title_short Binary Effects of Gynostemma Gold Nanoparticles on Obesity and Inflammation via Downregulation of PPARγ/CEPBα and TNF-α Gene Expression
title_sort binary effects of gynostemma gold nanoparticles on obesity and inflammation via downregulation of pparγ/cepbα and tnf-α gene expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104634/
https://www.ncbi.nlm.nih.gov/pubmed/35566145
http://dx.doi.org/10.3390/molecules27092795
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