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AOM/DSS Induced Colitis-Associated Colorectal Cancer in 14-Month-Old Female Balb/C and C57/Bl6 Mice—A Pilot Study

Colitis is a major risk factor for the development of colorectal cancer, leading to colitis-associated colorectal cancer (CAC). The most commonly used animal model to study CAC is the azoxymethane-dextran sulphate-sodium (AOM/DSS) model. The ideal experimental conditions of this model depend on seve...

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Autores principales: Schepelmann, Martin, Kupper, Nadja, Gushchina, Valeriya, Mesteri, Ildiko, Manhardt, Teresa, Moritsch, Stefan, Müller, Christian, Piatek, Karina, Salzmann, Martina, Vlasaty, Andrea, Eferl, Robert, Kallay, Enikö
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104960/
https://www.ncbi.nlm.nih.gov/pubmed/35563665
http://dx.doi.org/10.3390/ijms23095278
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author Schepelmann, Martin
Kupper, Nadja
Gushchina, Valeriya
Mesteri, Ildiko
Manhardt, Teresa
Moritsch, Stefan
Müller, Christian
Piatek, Karina
Salzmann, Martina
Vlasaty, Andrea
Eferl, Robert
Kallay, Enikö
author_facet Schepelmann, Martin
Kupper, Nadja
Gushchina, Valeriya
Mesteri, Ildiko
Manhardt, Teresa
Moritsch, Stefan
Müller, Christian
Piatek, Karina
Salzmann, Martina
Vlasaty, Andrea
Eferl, Robert
Kallay, Enikö
author_sort Schepelmann, Martin
collection PubMed
description Colitis is a major risk factor for the development of colorectal cancer, leading to colitis-associated colorectal cancer (CAC). The most commonly used animal model to study CAC is the azoxymethane-dextran sulphate-sodium (AOM/DSS) model. The ideal experimental conditions of this model depend on several factors, including the used mouse strain. No data on feasibility and conditions for older mice, e.g., for aging studies, have yet been reported. Thus, we conducted a descriptive, observational pilot study where CAC was induced in 14-month-old female Balb/C and C57/Bl6 mice using 12.5 mg/kg AOM i.p. and three different concentrations of DSS (1, 2, and 3%) in drinking water (ad. lib.). The mice were monitored regularly during the three-month experimental phase. After euthanasia, the colons of the mice were evaluated macroscopically and microscopically. Both the mouse strains showed a DSS-concentration-dependent induction of CAC. Carcinomas were only observed at 3% DSS. The DSS dose was found to be significantly correlated with the histology score and % Ki67 positive cells only in C57/Bl6 mice but not in Balb/C mice, which showed a variable response to the CAC induction. No differences in colon length, weight, or mucin content were observed. Optimal conditions for CAC induction in these aged animals are thus considered to be 3% DSS, as carcinomas did not develop when 2% DSS was used. On the other hand, Balb/C mice reacted severely to 3% DSS, indicating that 2.5% DSS may be the “sweet spot” for future experiments comparing CAC in aged Balb/C and C57/Bl6 mice. This model will allow investigation of the effect of aging on CAC development and therapy.
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spelling pubmed-91049602022-05-14 AOM/DSS Induced Colitis-Associated Colorectal Cancer in 14-Month-Old Female Balb/C and C57/Bl6 Mice—A Pilot Study Schepelmann, Martin Kupper, Nadja Gushchina, Valeriya Mesteri, Ildiko Manhardt, Teresa Moritsch, Stefan Müller, Christian Piatek, Karina Salzmann, Martina Vlasaty, Andrea Eferl, Robert Kallay, Enikö Int J Mol Sci Brief Report Colitis is a major risk factor for the development of colorectal cancer, leading to colitis-associated colorectal cancer (CAC). The most commonly used animal model to study CAC is the azoxymethane-dextran sulphate-sodium (AOM/DSS) model. The ideal experimental conditions of this model depend on several factors, including the used mouse strain. No data on feasibility and conditions for older mice, e.g., for aging studies, have yet been reported. Thus, we conducted a descriptive, observational pilot study where CAC was induced in 14-month-old female Balb/C and C57/Bl6 mice using 12.5 mg/kg AOM i.p. and three different concentrations of DSS (1, 2, and 3%) in drinking water (ad. lib.). The mice were monitored regularly during the three-month experimental phase. After euthanasia, the colons of the mice were evaluated macroscopically and microscopically. Both the mouse strains showed a DSS-concentration-dependent induction of CAC. Carcinomas were only observed at 3% DSS. The DSS dose was found to be significantly correlated with the histology score and % Ki67 positive cells only in C57/Bl6 mice but not in Balb/C mice, which showed a variable response to the CAC induction. No differences in colon length, weight, or mucin content were observed. Optimal conditions for CAC induction in these aged animals are thus considered to be 3% DSS, as carcinomas did not develop when 2% DSS was used. On the other hand, Balb/C mice reacted severely to 3% DSS, indicating that 2.5% DSS may be the “sweet spot” for future experiments comparing CAC in aged Balb/C and C57/Bl6 mice. This model will allow investigation of the effect of aging on CAC development and therapy. MDPI 2022-05-09 /pmc/articles/PMC9104960/ /pubmed/35563665 http://dx.doi.org/10.3390/ijms23095278 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Schepelmann, Martin
Kupper, Nadja
Gushchina, Valeriya
Mesteri, Ildiko
Manhardt, Teresa
Moritsch, Stefan
Müller, Christian
Piatek, Karina
Salzmann, Martina
Vlasaty, Andrea
Eferl, Robert
Kallay, Enikö
AOM/DSS Induced Colitis-Associated Colorectal Cancer in 14-Month-Old Female Balb/C and C57/Bl6 Mice—A Pilot Study
title AOM/DSS Induced Colitis-Associated Colorectal Cancer in 14-Month-Old Female Balb/C and C57/Bl6 Mice—A Pilot Study
title_full AOM/DSS Induced Colitis-Associated Colorectal Cancer in 14-Month-Old Female Balb/C and C57/Bl6 Mice—A Pilot Study
title_fullStr AOM/DSS Induced Colitis-Associated Colorectal Cancer in 14-Month-Old Female Balb/C and C57/Bl6 Mice—A Pilot Study
title_full_unstemmed AOM/DSS Induced Colitis-Associated Colorectal Cancer in 14-Month-Old Female Balb/C and C57/Bl6 Mice—A Pilot Study
title_short AOM/DSS Induced Colitis-Associated Colorectal Cancer in 14-Month-Old Female Balb/C and C57/Bl6 Mice—A Pilot Study
title_sort aom/dss induced colitis-associated colorectal cancer in 14-month-old female balb/c and c57/bl6 mice—a pilot study
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104960/
https://www.ncbi.nlm.nih.gov/pubmed/35563665
http://dx.doi.org/10.3390/ijms23095278
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