Metabolomics Reveals the Effects of High Dietary Energy Density on the Metabolism of Transition Angus Cows

SIMPLE SUMMARY: The increase in the metabolic demand and the dramatically decreased feed intake of cows around parturition often cause a negative energy balance status in cows, which can cause metabolic disorders. Before parturition, dry matter intake of cows starts to decline, and this decline is practically unavoidable. Therefore, increasing the energy density of the diet is extremely important. We used untargeted metabolomics to reveal the effect of high dietary energy density on body metabolism and explore whether it can alleviate negative energy balance. Our research shows that feeding a high-energy diet could significantly improve antioxidant capacity, maintain phosphatidylcholine homeostasis and reduce the negative energy balance of cows by regulating lipid mobilization, muscle mobilization, and protein turnover. ABSTRACT: The diet energy level plays a vital role in the energy balance of transition cows. We investigated the effects of high dietary energy density on body metabolism. Twenty multiparous Angus cows were randomly assigned to two treatment groups (10 cows/treatment), one receiving a high-energy (HE) diet (NEm = 1.67 Mcal/kg of DM) and the other administered a control (CON) diet (NEm = 1.53 Mcal/kg of DM). The results indicated that feeding a high-energy diet resulted in higher plasma glucose concentration and lower concentrations of plasma NEFA and BHBA on d 14 relative to calving in the HE-fed cows compared to the CON-fed ones. The postpartum plasma levels of T-AOC were lower in cows that received the CON diet than in cows in the HE group, while the concentration of malondialdehyde (MDA) showed an opposite trend. Among the 51 significantly different metabolites, the concentrations of most identified fatty acids decreased in HE cows. The concentrations of inosine, glutamine, and citric acid were higher in HE-fed cows than in CON-fed cows. Enrichment analysis revealed that linoleic acid metabolism, valine, leucine as well as isoleucine biosynthesis, and glycerophospholipid metabolism were significantly enriched in the two groups.