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Chronic Experimental Model of TNBS-Induced Colitis to Study Inflammatory Bowel Disease
Background: Inflammatory bowel disease (IBD) is a world healthcare problem. In order to evaluate the effect of new pharmacological approaches for IBD, we aim to develop and validate chronic trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice. Methods: Experimental colitis was induced by the...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105049/ https://www.ncbi.nlm.nih.gov/pubmed/35563130 http://dx.doi.org/10.3390/ijms23094739 |
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author | Silva, Inês Solas, João Pinto, Rui Mateus, Vanessa |
author_facet | Silva, Inês Solas, João Pinto, Rui Mateus, Vanessa |
author_sort | Silva, Inês |
collection | PubMed |
description | Background: Inflammatory bowel disease (IBD) is a world healthcare problem. In order to evaluate the effect of new pharmacological approaches for IBD, we aim to develop and validate chronic trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice. Methods: Experimental colitis was induced by the rectal administration of multiple doses of TNBS in female CD-1 mice. The protocol was performed with six experimental groups, depending on the TNBS administration frequency, and two control groups (sham and ethanol groups). Results: The survival rate was 73.3% in the first three weeks and, from week 4 until the end of the experimental protocol, the mice’s survival remained unaltered at 70.9%. Fecal hemoglobin presented a progressive increase until week 4 (5.8 ± 0.3 µmol Hg/g feces, p < 0.0001) compared with the ethanol group, with no statistical differences to week 6. The highest level of tumor necrosis factor-α was observed on week 3; however, after week 4, a slight decrease in tumor necrosis factor-α concentration was verified, and the level was maintained until week 6 (71.3 ± 3.3 pg/mL and 72.7 ± 3.6 pg/mL, respectively). Conclusions: These findings allowed the verification of a stable pattern of clinical and inflammation signs after week 4, suggesting that the chronic model of TNBS-induced colitis develops in 4 weeks. |
format | Online Article Text |
id | pubmed-9105049 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91050492022-05-14 Chronic Experimental Model of TNBS-Induced Colitis to Study Inflammatory Bowel Disease Silva, Inês Solas, João Pinto, Rui Mateus, Vanessa Int J Mol Sci Article Background: Inflammatory bowel disease (IBD) is a world healthcare problem. In order to evaluate the effect of new pharmacological approaches for IBD, we aim to develop and validate chronic trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice. Methods: Experimental colitis was induced by the rectal administration of multiple doses of TNBS in female CD-1 mice. The protocol was performed with six experimental groups, depending on the TNBS administration frequency, and two control groups (sham and ethanol groups). Results: The survival rate was 73.3% in the first three weeks and, from week 4 until the end of the experimental protocol, the mice’s survival remained unaltered at 70.9%. Fecal hemoglobin presented a progressive increase until week 4 (5.8 ± 0.3 µmol Hg/g feces, p < 0.0001) compared with the ethanol group, with no statistical differences to week 6. The highest level of tumor necrosis factor-α was observed on week 3; however, after week 4, a slight decrease in tumor necrosis factor-α concentration was verified, and the level was maintained until week 6 (71.3 ± 3.3 pg/mL and 72.7 ± 3.6 pg/mL, respectively). Conclusions: These findings allowed the verification of a stable pattern of clinical and inflammation signs after week 4, suggesting that the chronic model of TNBS-induced colitis develops in 4 weeks. MDPI 2022-04-25 /pmc/articles/PMC9105049/ /pubmed/35563130 http://dx.doi.org/10.3390/ijms23094739 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Silva, Inês Solas, João Pinto, Rui Mateus, Vanessa Chronic Experimental Model of TNBS-Induced Colitis to Study Inflammatory Bowel Disease |
title | Chronic Experimental Model of TNBS-Induced Colitis to Study Inflammatory Bowel Disease |
title_full | Chronic Experimental Model of TNBS-Induced Colitis to Study Inflammatory Bowel Disease |
title_fullStr | Chronic Experimental Model of TNBS-Induced Colitis to Study Inflammatory Bowel Disease |
title_full_unstemmed | Chronic Experimental Model of TNBS-Induced Colitis to Study Inflammatory Bowel Disease |
title_short | Chronic Experimental Model of TNBS-Induced Colitis to Study Inflammatory Bowel Disease |
title_sort | chronic experimental model of tnbs-induced colitis to study inflammatory bowel disease |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105049/ https://www.ncbi.nlm.nih.gov/pubmed/35563130 http://dx.doi.org/10.3390/ijms23094739 |
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