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Chronic Experimental Model of TNBS-Induced Colitis to Study Inflammatory Bowel Disease

Background: Inflammatory bowel disease (IBD) is a world healthcare problem. In order to evaluate the effect of new pharmacological approaches for IBD, we aim to develop and validate chronic trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice. Methods: Experimental colitis was induced by the...

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Autores principales: Silva, Inês, Solas, João, Pinto, Rui, Mateus, Vanessa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105049/
https://www.ncbi.nlm.nih.gov/pubmed/35563130
http://dx.doi.org/10.3390/ijms23094739
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author Silva, Inês
Solas, João
Pinto, Rui
Mateus, Vanessa
author_facet Silva, Inês
Solas, João
Pinto, Rui
Mateus, Vanessa
author_sort Silva, Inês
collection PubMed
description Background: Inflammatory bowel disease (IBD) is a world healthcare problem. In order to evaluate the effect of new pharmacological approaches for IBD, we aim to develop and validate chronic trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice. Methods: Experimental colitis was induced by the rectal administration of multiple doses of TNBS in female CD-1 mice. The protocol was performed with six experimental groups, depending on the TNBS administration frequency, and two control groups (sham and ethanol groups). Results: The survival rate was 73.3% in the first three weeks and, from week 4 until the end of the experimental protocol, the mice’s survival remained unaltered at 70.9%. Fecal hemoglobin presented a progressive increase until week 4 (5.8 ± 0.3 µmol Hg/g feces, p < 0.0001) compared with the ethanol group, with no statistical differences to week 6. The highest level of tumor necrosis factor-α was observed on week 3; however, after week 4, a slight decrease in tumor necrosis factor-α concentration was verified, and the level was maintained until week 6 (71.3 ± 3.3 pg/mL and 72.7 ± 3.6 pg/mL, respectively). Conclusions: These findings allowed the verification of a stable pattern of clinical and inflammation signs after week 4, suggesting that the chronic model of TNBS-induced colitis develops in 4 weeks.
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spelling pubmed-91050492022-05-14 Chronic Experimental Model of TNBS-Induced Colitis to Study Inflammatory Bowel Disease Silva, Inês Solas, João Pinto, Rui Mateus, Vanessa Int J Mol Sci Article Background: Inflammatory bowel disease (IBD) is a world healthcare problem. In order to evaluate the effect of new pharmacological approaches for IBD, we aim to develop and validate chronic trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice. Methods: Experimental colitis was induced by the rectal administration of multiple doses of TNBS in female CD-1 mice. The protocol was performed with six experimental groups, depending on the TNBS administration frequency, and two control groups (sham and ethanol groups). Results: The survival rate was 73.3% in the first three weeks and, from week 4 until the end of the experimental protocol, the mice’s survival remained unaltered at 70.9%. Fecal hemoglobin presented a progressive increase until week 4 (5.8 ± 0.3 µmol Hg/g feces, p < 0.0001) compared with the ethanol group, with no statistical differences to week 6. The highest level of tumor necrosis factor-α was observed on week 3; however, after week 4, a slight decrease in tumor necrosis factor-α concentration was verified, and the level was maintained until week 6 (71.3 ± 3.3 pg/mL and 72.7 ± 3.6 pg/mL, respectively). Conclusions: These findings allowed the verification of a stable pattern of clinical and inflammation signs after week 4, suggesting that the chronic model of TNBS-induced colitis develops in 4 weeks. MDPI 2022-04-25 /pmc/articles/PMC9105049/ /pubmed/35563130 http://dx.doi.org/10.3390/ijms23094739 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Silva, Inês
Solas, João
Pinto, Rui
Mateus, Vanessa
Chronic Experimental Model of TNBS-Induced Colitis to Study Inflammatory Bowel Disease
title Chronic Experimental Model of TNBS-Induced Colitis to Study Inflammatory Bowel Disease
title_full Chronic Experimental Model of TNBS-Induced Colitis to Study Inflammatory Bowel Disease
title_fullStr Chronic Experimental Model of TNBS-Induced Colitis to Study Inflammatory Bowel Disease
title_full_unstemmed Chronic Experimental Model of TNBS-Induced Colitis to Study Inflammatory Bowel Disease
title_short Chronic Experimental Model of TNBS-Induced Colitis to Study Inflammatory Bowel Disease
title_sort chronic experimental model of tnbs-induced colitis to study inflammatory bowel disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105049/
https://www.ncbi.nlm.nih.gov/pubmed/35563130
http://dx.doi.org/10.3390/ijms23094739
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