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Nuclear Lipid Droplet Birth during Replicative Stress
The nuclear membrane defines the boundaries that confine, protect and shape the genome. As such, its blebbing, ruptures and deformations are known to compromise the integrity of genetic material. Yet, drastic transitions of the nuclear membrane such as its invagination towards the nucleoplasm or its...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105094/ https://www.ncbi.nlm.nih.gov/pubmed/35563696 http://dx.doi.org/10.3390/cells11091390 |
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author | Kumanski, Sylvain Forey, Romain Cazevieille, Chantal Moriel-Carretero, María |
author_facet | Kumanski, Sylvain Forey, Romain Cazevieille, Chantal Moriel-Carretero, María |
author_sort | Kumanski, Sylvain |
collection | PubMed |
description | The nuclear membrane defines the boundaries that confine, protect and shape the genome. As such, its blebbing, ruptures and deformations are known to compromise the integrity of genetic material. Yet, drastic transitions of the nuclear membrane such as its invagination towards the nucleoplasm or its capacity to emit nuclear lipid droplets (nLD) have not been evaluated with respect to their impact on genome dynamics. To begin assessing this, in this work we used Saccharomyces cerevisiae as a model to ask whether a selection of genotoxins can trigger the formation of nLD. We report that nLD formation is not a general feature of all genotoxins, but of those engendering replication stress. Exacerbation of endogenous replication stress by genetic tools also elicited nLD formation. When exploring the lipid features of the nuclear membrane at the base of this emission, we revealed a link with the unsaturation profile of its phospholipids and, for the first time, of its sterol content. We propose that stressed replication forks may stimulate nLD birth by anchoring to the inner nuclear membrane, provided that the lipid context is adequate. Further, we point to a transcriptional feed-back process that counteracts the membrane’s proneness to emit nLD. With nLD representing platforms onto which genome-modifying reactions can occur, our findings highlight them as important players in the response to replication stress. |
format | Online Article Text |
id | pubmed-9105094 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91050942022-05-14 Nuclear Lipid Droplet Birth during Replicative Stress Kumanski, Sylvain Forey, Romain Cazevieille, Chantal Moriel-Carretero, María Cells Article The nuclear membrane defines the boundaries that confine, protect and shape the genome. As such, its blebbing, ruptures and deformations are known to compromise the integrity of genetic material. Yet, drastic transitions of the nuclear membrane such as its invagination towards the nucleoplasm or its capacity to emit nuclear lipid droplets (nLD) have not been evaluated with respect to their impact on genome dynamics. To begin assessing this, in this work we used Saccharomyces cerevisiae as a model to ask whether a selection of genotoxins can trigger the formation of nLD. We report that nLD formation is not a general feature of all genotoxins, but of those engendering replication stress. Exacerbation of endogenous replication stress by genetic tools also elicited nLD formation. When exploring the lipid features of the nuclear membrane at the base of this emission, we revealed a link with the unsaturation profile of its phospholipids and, for the first time, of its sterol content. We propose that stressed replication forks may stimulate nLD birth by anchoring to the inner nuclear membrane, provided that the lipid context is adequate. Further, we point to a transcriptional feed-back process that counteracts the membrane’s proneness to emit nLD. With nLD representing platforms onto which genome-modifying reactions can occur, our findings highlight them as important players in the response to replication stress. MDPI 2022-04-20 /pmc/articles/PMC9105094/ /pubmed/35563696 http://dx.doi.org/10.3390/cells11091390 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kumanski, Sylvain Forey, Romain Cazevieille, Chantal Moriel-Carretero, María Nuclear Lipid Droplet Birth during Replicative Stress |
title | Nuclear Lipid Droplet Birth during Replicative Stress |
title_full | Nuclear Lipid Droplet Birth during Replicative Stress |
title_fullStr | Nuclear Lipid Droplet Birth during Replicative Stress |
title_full_unstemmed | Nuclear Lipid Droplet Birth during Replicative Stress |
title_short | Nuclear Lipid Droplet Birth during Replicative Stress |
title_sort | nuclear lipid droplet birth during replicative stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105094/ https://www.ncbi.nlm.nih.gov/pubmed/35563696 http://dx.doi.org/10.3390/cells11091390 |
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