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Role of LOC_Os01g68450, Containing DUF2358, in Salt Tolerance Is Mediated via Adaptation of Absorbed Light Energy Dissipation

Salt stress affects plant growth and productivity. In this study we determined the roles of eight genes involved in photosynthesis, using gene co-expression network analysis, under salt-stress conditions using Arabidopsis knockout mutants. The green area of the leaves was minimum in the at1g65230 mu...

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Autores principales: Punchkhon, Chutarat, Chutimanukul, Panita, Chokwiwatkul, Ratchata, Saputro, Triono Bagus, Grennan, Aleel K., Diego, Nuria De, Spíchal, Lukáš, Chadchawan, Supachitra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105198/
https://www.ncbi.nlm.nih.gov/pubmed/35567234
http://dx.doi.org/10.3390/plants11091233
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author Punchkhon, Chutarat
Chutimanukul, Panita
Chokwiwatkul, Ratchata
Saputro, Triono Bagus
Grennan, Aleel K.
Diego, Nuria De
Spíchal, Lukáš
Chadchawan, Supachitra
author_facet Punchkhon, Chutarat
Chutimanukul, Panita
Chokwiwatkul, Ratchata
Saputro, Triono Bagus
Grennan, Aleel K.
Diego, Nuria De
Spíchal, Lukáš
Chadchawan, Supachitra
author_sort Punchkhon, Chutarat
collection PubMed
description Salt stress affects plant growth and productivity. In this study we determined the roles of eight genes involved in photosynthesis, using gene co-expression network analysis, under salt-stress conditions using Arabidopsis knockout mutants. The green area of the leaves was minimum in the at1g65230 mutant line. Rice LOC_Os01g68450, a homolog of at1g65230, was ectopically expressed in the at1g65230 mutant line to generate revertant lines. Under salt stress, the revertant lines exhibited significantly higher net photosynthesis rates than the at1g65230 mutant line. Moreover, the operating efficiency of photosystem II (PSII) and electron transport rate of the revertant lines were higher than those of the wild type and at1g65230 mutant line after 10 days of exposure to salt stress. After this period, the protein PsbD–the component of PSII–decreased in all lines tested without significant difference among them. However, the chlorophyll a and b, carotenoid, and anthocyanin contents of revertant lines were higher than those of the mutant line. Furthermore, lower maximum chlorophyll fluorescence was detected in the revertant lines. This suggests that LOC_Os01g68450 expression contributed to the salt tolerance phenotype by modifying the energy dissipation process and led to the ability to maintain photosynthesis under salt stress conditions.
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spelling pubmed-91051982022-05-14 Role of LOC_Os01g68450, Containing DUF2358, in Salt Tolerance Is Mediated via Adaptation of Absorbed Light Energy Dissipation Punchkhon, Chutarat Chutimanukul, Panita Chokwiwatkul, Ratchata Saputro, Triono Bagus Grennan, Aleel K. Diego, Nuria De Spíchal, Lukáš Chadchawan, Supachitra Plants (Basel) Article Salt stress affects plant growth and productivity. In this study we determined the roles of eight genes involved in photosynthesis, using gene co-expression network analysis, under salt-stress conditions using Arabidopsis knockout mutants. The green area of the leaves was minimum in the at1g65230 mutant line. Rice LOC_Os01g68450, a homolog of at1g65230, was ectopically expressed in the at1g65230 mutant line to generate revertant lines. Under salt stress, the revertant lines exhibited significantly higher net photosynthesis rates than the at1g65230 mutant line. Moreover, the operating efficiency of photosystem II (PSII) and electron transport rate of the revertant lines were higher than those of the wild type and at1g65230 mutant line after 10 days of exposure to salt stress. After this period, the protein PsbD–the component of PSII–decreased in all lines tested without significant difference among them. However, the chlorophyll a and b, carotenoid, and anthocyanin contents of revertant lines were higher than those of the mutant line. Furthermore, lower maximum chlorophyll fluorescence was detected in the revertant lines. This suggests that LOC_Os01g68450 expression contributed to the salt tolerance phenotype by modifying the energy dissipation process and led to the ability to maintain photosynthesis under salt stress conditions. MDPI 2022-05-02 /pmc/articles/PMC9105198/ /pubmed/35567234 http://dx.doi.org/10.3390/plants11091233 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Punchkhon, Chutarat
Chutimanukul, Panita
Chokwiwatkul, Ratchata
Saputro, Triono Bagus
Grennan, Aleel K.
Diego, Nuria De
Spíchal, Lukáš
Chadchawan, Supachitra
Role of LOC_Os01g68450, Containing DUF2358, in Salt Tolerance Is Mediated via Adaptation of Absorbed Light Energy Dissipation
title Role of LOC_Os01g68450, Containing DUF2358, in Salt Tolerance Is Mediated via Adaptation of Absorbed Light Energy Dissipation
title_full Role of LOC_Os01g68450, Containing DUF2358, in Salt Tolerance Is Mediated via Adaptation of Absorbed Light Energy Dissipation
title_fullStr Role of LOC_Os01g68450, Containing DUF2358, in Salt Tolerance Is Mediated via Adaptation of Absorbed Light Energy Dissipation
title_full_unstemmed Role of LOC_Os01g68450, Containing DUF2358, in Salt Tolerance Is Mediated via Adaptation of Absorbed Light Energy Dissipation
title_short Role of LOC_Os01g68450, Containing DUF2358, in Salt Tolerance Is Mediated via Adaptation of Absorbed Light Energy Dissipation
title_sort role of loc_os01g68450, containing duf2358, in salt tolerance is mediated via adaptation of absorbed light energy dissipation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105198/
https://www.ncbi.nlm.nih.gov/pubmed/35567234
http://dx.doi.org/10.3390/plants11091233
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