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Visualization of the Crossroads between a Nascent Infection Thread and the First Cell Division Event in Phaseolus vulgaris Nodulation

The development of a symbiotic nitrogen-fixing nodule in legumes involves infection and organogenesis. Infection begins when rhizobia enter a root hair through an inward structure, the infection thread (IT), which guides the bacteria towards the cortical tissue. Concurrently, organogenesis takes pla...

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Autores principales: Monroy-Morales, Elizabeth, Dávila-Delgado, Raúl, Ayala-Guzmán, Emmanuel, Gamboa-deBuen, Alicia, Sánchez-López, Rosana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105610/
https://www.ncbi.nlm.nih.gov/pubmed/35563659
http://dx.doi.org/10.3390/ijms23095267
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author Monroy-Morales, Elizabeth
Dávila-Delgado, Raúl
Ayala-Guzmán, Emmanuel
Gamboa-deBuen, Alicia
Sánchez-López, Rosana
author_facet Monroy-Morales, Elizabeth
Dávila-Delgado, Raúl
Ayala-Guzmán, Emmanuel
Gamboa-deBuen, Alicia
Sánchez-López, Rosana
author_sort Monroy-Morales, Elizabeth
collection PubMed
description The development of a symbiotic nitrogen-fixing nodule in legumes involves infection and organogenesis. Infection begins when rhizobia enter a root hair through an inward structure, the infection thread (IT), which guides the bacteria towards the cortical tissue. Concurrently, organogenesis takes place by inducing cortical cell division (CCD) at the infection site. Genetic analysis showed that both events are well-coordinated; however, the dynamics connecting them remain to be elucidated. To visualize the crossroads between IT and CCD, we benefited from the fact that, in Phaseolus vulgaris nodulation, where the first division occurs in subepidermal cortical cells located underneath the infection site, we traced a Rhizobium etli strain expressing DsRed, the plant cytokinesis marker YFP-PvKNOLLE, a nuclear stain and cell wall auto-fluorescence. We found that the IT exits the root hair to penetrate an underlying subepidermal cortical (S-E) cell when it is concluding cytokinesis.
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spelling pubmed-91056102022-05-14 Visualization of the Crossroads between a Nascent Infection Thread and the First Cell Division Event in Phaseolus vulgaris Nodulation Monroy-Morales, Elizabeth Dávila-Delgado, Raúl Ayala-Guzmán, Emmanuel Gamboa-deBuen, Alicia Sánchez-López, Rosana Int J Mol Sci Article The development of a symbiotic nitrogen-fixing nodule in legumes involves infection and organogenesis. Infection begins when rhizobia enter a root hair through an inward structure, the infection thread (IT), which guides the bacteria towards the cortical tissue. Concurrently, organogenesis takes place by inducing cortical cell division (CCD) at the infection site. Genetic analysis showed that both events are well-coordinated; however, the dynamics connecting them remain to be elucidated. To visualize the crossroads between IT and CCD, we benefited from the fact that, in Phaseolus vulgaris nodulation, where the first division occurs in subepidermal cortical cells located underneath the infection site, we traced a Rhizobium etli strain expressing DsRed, the plant cytokinesis marker YFP-PvKNOLLE, a nuclear stain and cell wall auto-fluorescence. We found that the IT exits the root hair to penetrate an underlying subepidermal cortical (S-E) cell when it is concluding cytokinesis. MDPI 2022-05-09 /pmc/articles/PMC9105610/ /pubmed/35563659 http://dx.doi.org/10.3390/ijms23095267 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Monroy-Morales, Elizabeth
Dávila-Delgado, Raúl
Ayala-Guzmán, Emmanuel
Gamboa-deBuen, Alicia
Sánchez-López, Rosana
Visualization of the Crossroads between a Nascent Infection Thread and the First Cell Division Event in Phaseolus vulgaris Nodulation
title Visualization of the Crossroads between a Nascent Infection Thread and the First Cell Division Event in Phaseolus vulgaris Nodulation
title_full Visualization of the Crossroads between a Nascent Infection Thread and the First Cell Division Event in Phaseolus vulgaris Nodulation
title_fullStr Visualization of the Crossroads between a Nascent Infection Thread and the First Cell Division Event in Phaseolus vulgaris Nodulation
title_full_unstemmed Visualization of the Crossroads between a Nascent Infection Thread and the First Cell Division Event in Phaseolus vulgaris Nodulation
title_short Visualization of the Crossroads between a Nascent Infection Thread and the First Cell Division Event in Phaseolus vulgaris Nodulation
title_sort visualization of the crossroads between a nascent infection thread and the first cell division event in phaseolus vulgaris nodulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105610/
https://www.ncbi.nlm.nih.gov/pubmed/35563659
http://dx.doi.org/10.3390/ijms23095267
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