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Populus euphratica Phospholipase Dδ Increases Salt Tolerance by Regulating K(+)/Na(+) and ROS Homeostasis in Arabidopsis

Phospholipase Dα (PLDα), which produces signaling molecules phosphatidic acid (PA), has been shown to play a critical role in plants adapting to salt environments. However, it is unclear whether phospholipase Dδ (PLDδ) can mediate the salt response in higher plants. PePLDδ was isolated from salt-res...

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Autores principales: Zhang, Ying, Yao, Jun, Yin, Kexin, Liu, Zhe, Zhang, Yanli, Deng, Chen, Liu, Jian, Zhang, Yinan, Hou, Siyuan, Zhang, Huilong, Yu, Dade, Zhao, Nan, Zhao, Rui, Chen, Shaoliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105705/
https://www.ncbi.nlm.nih.gov/pubmed/35563299
http://dx.doi.org/10.3390/ijms23094911
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author Zhang, Ying
Yao, Jun
Yin, Kexin
Liu, Zhe
Zhang, Yanli
Deng, Chen
Liu, Jian
Zhang, Yinan
Hou, Siyuan
Zhang, Huilong
Yu, Dade
Zhao, Nan
Zhao, Rui
Chen, Shaoliang
author_facet Zhang, Ying
Yao, Jun
Yin, Kexin
Liu, Zhe
Zhang, Yanli
Deng, Chen
Liu, Jian
Zhang, Yinan
Hou, Siyuan
Zhang, Huilong
Yu, Dade
Zhao, Nan
Zhao, Rui
Chen, Shaoliang
author_sort Zhang, Ying
collection PubMed
description Phospholipase Dα (PLDα), which produces signaling molecules phosphatidic acid (PA), has been shown to play a critical role in plants adapting to salt environments. However, it is unclear whether phospholipase Dδ (PLDδ) can mediate the salt response in higher plants. PePLDδ was isolated from salt-resistant Populus euphratica and transferred to Arabidopsis thaliana to testify the salt tolerance of transgenic plants. The NaCl treatment (130 mM) reduced the root growth and whole-plant fresh weight of wild-type (WT) A. thaliana, vector controls (VC) and PePLDδ-overexpressed lines, although a less pronounced effect was observed in transgenic plants. Under salt treatment, PePLDδ-transgenic Arabidopsis exhibited lower electrolyte leakage, malondialdehyde content and H(2)O(2) levels than WT and VC, resulting from the activated antioxidant enzymes and upregulated transcripts of genes encoding superoxide dismutase, ascorbic acid peroxidase and peroxidase. In addition, PePLDδ-overexpressed plants increased the transcription of genes encoding the plasma membrane Na(+)/H(+) antiporter (AtSOS1) and H(+)-ATPase (AtAHA2), which enabled transgenic plants to proceed with Na(+) extrusion and reduce K(+) loss under salinity. The capacity to regulate reactive oxygen species (ROS) and K(+)/Na(+) homeostasis was associated with the abundance of specific PA species in plants overexpressing PePLDδ. PePLDδ-transgenic plants retained a typically higher abundance of PA species, 34:2 (16:0–18:2), 34:3 (16:0–18:3), 36:4 (18:2–18:2), 36:5 (18:2–18:3) and 36:6 (18:3–18:3), under control and saline conditions. It is noteworthy that PA species 34:2 (16:0–18:2), 34:3 (16:0–18:3), 36:4 (18:2–18:2) and 36:5 (18:2–18:3) markedly increased in response to NaCl in transgenic plants. In conclusion, we suppose that PePLDδ-derived PA enhanced the salinity tolerance by regulating ROS and K(+)/Na(+) homeostasis in Arabidopsis.
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spelling pubmed-91057052022-05-14 Populus euphratica Phospholipase Dδ Increases Salt Tolerance by Regulating K(+)/Na(+) and ROS Homeostasis in Arabidopsis Zhang, Ying Yao, Jun Yin, Kexin Liu, Zhe Zhang, Yanli Deng, Chen Liu, Jian Zhang, Yinan Hou, Siyuan Zhang, Huilong Yu, Dade Zhao, Nan Zhao, Rui Chen, Shaoliang Int J Mol Sci Article Phospholipase Dα (PLDα), which produces signaling molecules phosphatidic acid (PA), has been shown to play a critical role in plants adapting to salt environments. However, it is unclear whether phospholipase Dδ (PLDδ) can mediate the salt response in higher plants. PePLDδ was isolated from salt-resistant Populus euphratica and transferred to Arabidopsis thaliana to testify the salt tolerance of transgenic plants. The NaCl treatment (130 mM) reduced the root growth and whole-plant fresh weight of wild-type (WT) A. thaliana, vector controls (VC) and PePLDδ-overexpressed lines, although a less pronounced effect was observed in transgenic plants. Under salt treatment, PePLDδ-transgenic Arabidopsis exhibited lower electrolyte leakage, malondialdehyde content and H(2)O(2) levels than WT and VC, resulting from the activated antioxidant enzymes and upregulated transcripts of genes encoding superoxide dismutase, ascorbic acid peroxidase and peroxidase. In addition, PePLDδ-overexpressed plants increased the transcription of genes encoding the plasma membrane Na(+)/H(+) antiporter (AtSOS1) and H(+)-ATPase (AtAHA2), which enabled transgenic plants to proceed with Na(+) extrusion and reduce K(+) loss under salinity. The capacity to regulate reactive oxygen species (ROS) and K(+)/Na(+) homeostasis was associated with the abundance of specific PA species in plants overexpressing PePLDδ. PePLDδ-transgenic plants retained a typically higher abundance of PA species, 34:2 (16:0–18:2), 34:3 (16:0–18:3), 36:4 (18:2–18:2), 36:5 (18:2–18:3) and 36:6 (18:3–18:3), under control and saline conditions. It is noteworthy that PA species 34:2 (16:0–18:2), 34:3 (16:0–18:3), 36:4 (18:2–18:2) and 36:5 (18:2–18:3) markedly increased in response to NaCl in transgenic plants. In conclusion, we suppose that PePLDδ-derived PA enhanced the salinity tolerance by regulating ROS and K(+)/Na(+) homeostasis in Arabidopsis. MDPI 2022-04-28 /pmc/articles/PMC9105705/ /pubmed/35563299 http://dx.doi.org/10.3390/ijms23094911 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Ying
Yao, Jun
Yin, Kexin
Liu, Zhe
Zhang, Yanli
Deng, Chen
Liu, Jian
Zhang, Yinan
Hou, Siyuan
Zhang, Huilong
Yu, Dade
Zhao, Nan
Zhao, Rui
Chen, Shaoliang
Populus euphratica Phospholipase Dδ Increases Salt Tolerance by Regulating K(+)/Na(+) and ROS Homeostasis in Arabidopsis
title Populus euphratica Phospholipase Dδ Increases Salt Tolerance by Regulating K(+)/Na(+) and ROS Homeostasis in Arabidopsis
title_full Populus euphratica Phospholipase Dδ Increases Salt Tolerance by Regulating K(+)/Na(+) and ROS Homeostasis in Arabidopsis
title_fullStr Populus euphratica Phospholipase Dδ Increases Salt Tolerance by Regulating K(+)/Na(+) and ROS Homeostasis in Arabidopsis
title_full_unstemmed Populus euphratica Phospholipase Dδ Increases Salt Tolerance by Regulating K(+)/Na(+) and ROS Homeostasis in Arabidopsis
title_short Populus euphratica Phospholipase Dδ Increases Salt Tolerance by Regulating K(+)/Na(+) and ROS Homeostasis in Arabidopsis
title_sort populus euphratica phospholipase dδ increases salt tolerance by regulating k(+)/na(+) and ros homeostasis in arabidopsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105705/
https://www.ncbi.nlm.nih.gov/pubmed/35563299
http://dx.doi.org/10.3390/ijms23094911
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