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Compound-Specific (14)N/(15)N Analysis of Amino Acid Trimethylsilylated Derivatives from Plant Seed Proteins
Isotopic analyses of plant samples are now of considerable importance for food certification and plant physiology. In fact, the natural nitrogen isotope composition (δ(15)N) is extremely useful to examine metabolic pathways of N nutrition involving isotope fractionations. However, δ(15)N analysis of...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105707/ https://www.ncbi.nlm.nih.gov/pubmed/35563286 http://dx.doi.org/10.3390/ijms23094893 |
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author | Domergue, Jean-Baptiste Lalande, Julie Abadie, Cyril Tcherkez, Guillaume |
author_facet | Domergue, Jean-Baptiste Lalande, Julie Abadie, Cyril Tcherkez, Guillaume |
author_sort | Domergue, Jean-Baptiste |
collection | PubMed |
description | Isotopic analyses of plant samples are now of considerable importance for food certification and plant physiology. In fact, the natural nitrogen isotope composition (δ(15)N) is extremely useful to examine metabolic pathways of N nutrition involving isotope fractionations. However, δ(15)N analysis of amino acids is not straightforward and involves specific derivatization procedures to yield volatile derivatives that can be analysed by gas chromatography coupled to isotope ratio mass spectrometry (GC-C-IRMS). Derivatizations other than trimethylsilylation are commonly used since they are believed to be more reliable and accurate. Their major drawback is that they are not associated with metabolite databases allowing identification of derivatives and by-products. Here, we revisit the potential of trimethylsilylated derivatives via concurrent analysis of δ(15)N and exact mass GC-MS of plant seed protein samples, allowing facile identification of derivatives using a database used for metabolomics. When multiple silylated derivatives of several amino acids are accounted for, there is a good agreement between theoretical and observed N mole fractions, and δ(15)N values are satisfactory, with little fractionation during derivatization. Overall, this technique may be suitable for compound-specific δ(15)N analysis, with pros and cons. |
format | Online Article Text |
id | pubmed-9105707 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91057072022-05-14 Compound-Specific (14)N/(15)N Analysis of Amino Acid Trimethylsilylated Derivatives from Plant Seed Proteins Domergue, Jean-Baptiste Lalande, Julie Abadie, Cyril Tcherkez, Guillaume Int J Mol Sci Article Isotopic analyses of plant samples are now of considerable importance for food certification and plant physiology. In fact, the natural nitrogen isotope composition (δ(15)N) is extremely useful to examine metabolic pathways of N nutrition involving isotope fractionations. However, δ(15)N analysis of amino acids is not straightforward and involves specific derivatization procedures to yield volatile derivatives that can be analysed by gas chromatography coupled to isotope ratio mass spectrometry (GC-C-IRMS). Derivatizations other than trimethylsilylation are commonly used since they are believed to be more reliable and accurate. Their major drawback is that they are not associated with metabolite databases allowing identification of derivatives and by-products. Here, we revisit the potential of trimethylsilylated derivatives via concurrent analysis of δ(15)N and exact mass GC-MS of plant seed protein samples, allowing facile identification of derivatives using a database used for metabolomics. When multiple silylated derivatives of several amino acids are accounted for, there is a good agreement between theoretical and observed N mole fractions, and δ(15)N values are satisfactory, with little fractionation during derivatization. Overall, this technique may be suitable for compound-specific δ(15)N analysis, with pros and cons. MDPI 2022-04-28 /pmc/articles/PMC9105707/ /pubmed/35563286 http://dx.doi.org/10.3390/ijms23094893 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Domergue, Jean-Baptiste Lalande, Julie Abadie, Cyril Tcherkez, Guillaume Compound-Specific (14)N/(15)N Analysis of Amino Acid Trimethylsilylated Derivatives from Plant Seed Proteins |
title | Compound-Specific (14)N/(15)N Analysis of Amino Acid Trimethylsilylated Derivatives from Plant Seed Proteins |
title_full | Compound-Specific (14)N/(15)N Analysis of Amino Acid Trimethylsilylated Derivatives from Plant Seed Proteins |
title_fullStr | Compound-Specific (14)N/(15)N Analysis of Amino Acid Trimethylsilylated Derivatives from Plant Seed Proteins |
title_full_unstemmed | Compound-Specific (14)N/(15)N Analysis of Amino Acid Trimethylsilylated Derivatives from Plant Seed Proteins |
title_short | Compound-Specific (14)N/(15)N Analysis of Amino Acid Trimethylsilylated Derivatives from Plant Seed Proteins |
title_sort | compound-specific (14)n/(15)n analysis of amino acid trimethylsilylated derivatives from plant seed proteins |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105707/ https://www.ncbi.nlm.nih.gov/pubmed/35563286 http://dx.doi.org/10.3390/ijms23094893 |
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