Postmortem Electrical Conductivity Changes of Dicentrarchus labrax Skeletal Muscle: Root Mean Square (RMS) Parameter in Estimating Time since Death

SIMPLE SUMMARY: The estimation of postmortem interval (PMI) still poses a major challenge for pathologists worldwide, making the search for new and more accurate technologies to assist in PMI estimation worthy of growing scientific interest. This study aimed to explore for the first time the use of an oscilloscope coupled with a signal generator, as innovative technology, to evaluate changes in the electrical conductivity of skeletal muscle of sea bass specimens during the early postmortem interval, to find an accurate, quantitative parameter useful in PMI estimation. The use of the oscilloscope, especially for the RMS measured parameter, has been shown here as a promising technology for studying dielectric muscle properties during the early postmortem interval, with the advantage of being a rapid, non-destructive, and inexpensive method. ABSTRACT: Electric impedance spectroscopy techniques have been widely employed to study basic biological processes, and recently explored to estimate postmortem interval (PMI). However, the most-relevant parameter to approximate PMI has not been recognized so far. This study investigated electrical conductivity changes in muscle of 18 sea bass specimens, maintained at different room temperatures (15.0 °C; 20.0 °C; 25.0 °C), during a 24 h postmortem period using an oscilloscope coupled with a signal generator, as innovative technology. The root mean square (RMS) was selected among all measured parameters, and recorded every 15 min for 24 h after death. The RMS(t) time series for each animal were collected and statistically analyzed using MATLAB(®). A similar trend in RMS values was observed in all animals over the 24 h study period. After a short period, during which the RMS signal decreased, an increasing trend of the signal was recorded for all fish until it reached a peak. Subsequently, the RMS value gradually decreased over time. A strong linear correlation was observed among the time series, confirming that the above time-behaviour holds for all animals. The time at which maximum value is reached strongly depended on the room temperature during the experiments, ranging from 6 h in fish kept at 25.0 °C to 14 h in animals kept at 15.0 °C. The use of the oscilloscope has proven to be a promising technology in the study of electrical muscle properties during the early postmortem interval, with the advantage of being a fast, non-destructive, and inexpensive method, although more studies will be needed to validate this technology before moving to real-time field investigations.