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Prognostic Significance of GPR55 mRNA Expression in Colon Cancer

G protein-coupled receptor 55 (GPR55) probably plays a role in innate immunity and tumor immunosurveillance through its effect on immune cells, such as T cells and NK cells. In this study, the prognostic value of GPR55 in colon cancer (CC) was investigated. mRNA expression levels of GPR55 were deter...

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Autores principales: Ismail, Hager Tarek H., AbdelMageed, Manar, Lindmark, Gudrun, Hammarström, Marie-Louise, Hammarström, Sten, Sitohy, Basel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106053/
https://www.ncbi.nlm.nih.gov/pubmed/35562947
http://dx.doi.org/10.3390/ijms23094556
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author Ismail, Hager Tarek H.
AbdelMageed, Manar
Lindmark, Gudrun
Hammarström, Marie-Louise
Hammarström, Sten
Sitohy, Basel
author_facet Ismail, Hager Tarek H.
AbdelMageed, Manar
Lindmark, Gudrun
Hammarström, Marie-Louise
Hammarström, Sten
Sitohy, Basel
author_sort Ismail, Hager Tarek H.
collection PubMed
description G protein-coupled receptor 55 (GPR55) probably plays a role in innate immunity and tumor immunosurveillance through its effect on immune cells, such as T cells and NK cells. In this study, the prognostic value of GPR55 in colon cancer (CC) was investigated. mRNA expression levels of GPR55 were determined in 382 regional lymph nodes of 121 CC patients with 12 years observation time after curative surgery. The same clinical material had previously been analyzed for expression levels of CEA, CXCL16, CXCL17, GPR35 V2/3 and LGR5 mRNAs. Clinical cutoffs of 0.1365 copies/18S rRNA unit for GPR55 and 0.1481 for the GPR55/CEA ratio were applied to differentiate between the high- and low-GPR55 expression groups. Kaplan–Meier survival analysis and Cox regression risk analysis were used to determine prognostic value. Improved discrimination between the two groups was achieved by combining GPR55 with CEA, CXCL16 or CXCL17 compared with GPR55 alone. The best result was obtained using the GPR55/CEA ratio, with an increased mean survival time of 14 and 33 months at 5 and 12 years observation time, respectively (p = 0.0003 and p = 0.003) for the high-GPR55/CEA group. The explanation for the observed improvement is most likely that GPR55 is a marker for T cells and B cells in lymph nodes, whereas CEA, CXCL16 and CXCL17, are markers for tumor cells of epithelial origin.
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spelling pubmed-91060532022-05-14 Prognostic Significance of GPR55 mRNA Expression in Colon Cancer Ismail, Hager Tarek H. AbdelMageed, Manar Lindmark, Gudrun Hammarström, Marie-Louise Hammarström, Sten Sitohy, Basel Int J Mol Sci Article G protein-coupled receptor 55 (GPR55) probably plays a role in innate immunity and tumor immunosurveillance through its effect on immune cells, such as T cells and NK cells. In this study, the prognostic value of GPR55 in colon cancer (CC) was investigated. mRNA expression levels of GPR55 were determined in 382 regional lymph nodes of 121 CC patients with 12 years observation time after curative surgery. The same clinical material had previously been analyzed for expression levels of CEA, CXCL16, CXCL17, GPR35 V2/3 and LGR5 mRNAs. Clinical cutoffs of 0.1365 copies/18S rRNA unit for GPR55 and 0.1481 for the GPR55/CEA ratio were applied to differentiate between the high- and low-GPR55 expression groups. Kaplan–Meier survival analysis and Cox regression risk analysis were used to determine prognostic value. Improved discrimination between the two groups was achieved by combining GPR55 with CEA, CXCL16 or CXCL17 compared with GPR55 alone. The best result was obtained using the GPR55/CEA ratio, with an increased mean survival time of 14 and 33 months at 5 and 12 years observation time, respectively (p = 0.0003 and p = 0.003) for the high-GPR55/CEA group. The explanation for the observed improvement is most likely that GPR55 is a marker for T cells and B cells in lymph nodes, whereas CEA, CXCL16 and CXCL17, are markers for tumor cells of epithelial origin. MDPI 2022-04-20 /pmc/articles/PMC9106053/ /pubmed/35562947 http://dx.doi.org/10.3390/ijms23094556 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ismail, Hager Tarek H.
AbdelMageed, Manar
Lindmark, Gudrun
Hammarström, Marie-Louise
Hammarström, Sten
Sitohy, Basel
Prognostic Significance of GPR55 mRNA Expression in Colon Cancer
title Prognostic Significance of GPR55 mRNA Expression in Colon Cancer
title_full Prognostic Significance of GPR55 mRNA Expression in Colon Cancer
title_fullStr Prognostic Significance of GPR55 mRNA Expression in Colon Cancer
title_full_unstemmed Prognostic Significance of GPR55 mRNA Expression in Colon Cancer
title_short Prognostic Significance of GPR55 mRNA Expression in Colon Cancer
title_sort prognostic significance of gpr55 mrna expression in colon cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106053/
https://www.ncbi.nlm.nih.gov/pubmed/35562947
http://dx.doi.org/10.3390/ijms23094556
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