Comparative Neuroanatomical Study of the Main Olfactory Bulb in Domestic and Wild Canids: Dog, Wolf and Red Fox

SIMPLE SUMMARY: The study of the morphological, physiological and molecular changes associated with the domestication process has been one of the most interesting unresolved neuroanatomical issues. The olfactory system deserves special attention since both wild and domestic canids are macrosmatic mammals with very high olfactory capacities. Nevertheless, the question remains open as to whether domestication involuted the sense of smell in domestic dogs. Further, there is a lack of comparative morphological information on the olfactory bulb, the first structure integrating olfactory sensory information in the brain. To provide comparative information on the domestication process, we studied the olfactory bulb of dogs and their two most important wild ancestors: the wolf and the fox. The study was carried out by macroscopic dissection and histological and immunohistochemical techniques and has allowed us to verify, first of all, that the three species present olfactory bulbs corresponding to a macrosmatic animal, but that there are noticeable differences not only in size, which was already known, but also in the cellularity and intensity of the immunohistochemical pattern characteristic of each species. These variations point to a reduction of the olfactory system as a consequence of the selection pressure associated with the domestication of animals. ABSTRACT: The sense of smell plays a fundamental role in mammalian survival. There is a considerable amount of information available on the vomeronasal system of both domestic and wild canids. However, much less information is available on the canid main olfactory system, particularly at the level of the main olfactory bulb. Comparative study of the neuroanatomy of wild and domestic canids provides an excellent model for understanding the effects of selection pressure associated with domestication. A comprehensive histological (hematoxylin–eosin, Nissl, Tolivia and Gallego’s Trichrome stains), lectin (UEA, LEA) and immunohistochemical (Gαo, Gαi2, calretinin, calbindin, olfactory marker protein, glial fibrillary acidic protein, microtubule-associated protein 2) study of the olfactory bulbs of the dog, fox and wolf was performed. Our study found greater macroscopic development of the olfactory bulb in both the wolf and fox compared to the dog. At the microscopic level, all three species show a well-developed pattern of lamination and cellularity typical of a macrosmatic animal. However, greater development of cellularity in the periglomerular and mitral layers of wild canids is characteristic. Likewise, the immunohistochemical study shows comparable results between the three species, but with a noticeably higher expression of markers in wild canids. These results suggest that the reduction in encephalization experienced in dogs due to domestication also corresponds to a lower degree of morphological and neurochemical differentiation of the olfactory bulb.