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Creatinine as a Urinary Marker of the Purine Derivatives Excretion in Urine Spot Samples of Lambs Fed Peach Palm Meal

SIMPLE SUMMARY: Experimental trials designed to predict the microbial protein synthesis in the rumen via urinary purine derivatives require access to 24-h urinary volumes. This technique is non-invasive but requires access to 24-h urinary volumes, which is impracticable in grazing animals or a large...

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Autores principales: Pereira, Taiala Cristina de Jesus, Pereira, Mara Lúcia Albuquerque, de Carvalho, Gleidson Giordano Pinto, Silva, Herymá Giovane de Oliveira, dos Santos, Alana Batista, Pina, Douglas dos Santos, Sousa, Leandro Borges
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106062/
https://www.ncbi.nlm.nih.gov/pubmed/35565619
http://dx.doi.org/10.3390/ani12091195
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author Pereira, Taiala Cristina de Jesus
Pereira, Mara Lúcia Albuquerque
de Carvalho, Gleidson Giordano Pinto
Silva, Herymá Giovane de Oliveira
dos Santos, Alana Batista
Pina, Douglas dos Santos
Sousa, Leandro Borges
author_facet Pereira, Taiala Cristina de Jesus
Pereira, Mara Lúcia Albuquerque
de Carvalho, Gleidson Giordano Pinto
Silva, Herymá Giovane de Oliveira
dos Santos, Alana Batista
Pina, Douglas dos Santos
Sousa, Leandro Borges
author_sort Pereira, Taiala Cristina de Jesus
collection PubMed
description SIMPLE SUMMARY: Experimental trials designed to predict the microbial protein synthesis in the rumen via urinary purine derivatives require access to 24-h urinary volumes. This technique is non-invasive but requires access to 24-h urinary volumes, which is impracticable in grazing animals or a large number of feedlot sheep. The challenge is to provide the possibility of using the purine derivatives and creatinine concentrations in a spot urine sample and the daily creatinine excretion in urine as accurate estimators of purine derivatives’ excretion per day. ABSTRACT: The objective was to evaluate the influence of diets on lambs using different levels of peach palm meal as a replacement for maize (0, 10, 40, 60, and 85% of diet dry matter) on the endogenous creatinine clearance (CC), urine concentration ratio of purine derivatives to creatinine (PDC index), and daily creatinine excretion (DCE) as a marker to estimate purine derivatives (PD) excretion from urinary spot samples collected at different time points (4, 8, 12, 16, 20, 24 h after morning feeding) compared to 24-h total urine collection. The measured parameters were voluntary intake, urinary volume, CC, DCE, the concentration of plasma creatinine, and PD and purine derivatives’ excretion (PDE). Five lambs were allocated to metabolic cages and distributed in a 5 × 5 Latin square. Urine collection was taken daily on days 16 to 19 of each experimental period. The inclusion of peach palm meal linearly reduced the intake of dry matter (g kg BW(−0.75), p = 0.005), crude protein (g kg BW(−0.75), p = 0.010), metabolizable energy (MJ kg BW(−0.75), p = 0.010) and CC (p < 0.0001). It also quadratically affected the urinary volume (p = 0.008) and DCE (p = 0.004). There was a linear decrease for PDC index (p = 0.032) and PDE (p < 0.0001) measured in the 24-h total urine with peach palm meal levels. The different times of spot urine sampling did not affect (p > 0.05) the PDC index and PDE. Peach palm meal decreases the CC thereby compromising the use of a mean value of DCE as a PDE marker in spot urine samples. There is greater accuracy when using different values of DCE obtained for each diet as markers for the PDE in spot urine samples. Unconventional foodstuffs of low palatability affecting the voluntary intake of feed change the renal function.
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spelling pubmed-91060622022-05-14 Creatinine as a Urinary Marker of the Purine Derivatives Excretion in Urine Spot Samples of Lambs Fed Peach Palm Meal Pereira, Taiala Cristina de Jesus Pereira, Mara Lúcia Albuquerque de Carvalho, Gleidson Giordano Pinto Silva, Herymá Giovane de Oliveira dos Santos, Alana Batista Pina, Douglas dos Santos Sousa, Leandro Borges Animals (Basel) Article SIMPLE SUMMARY: Experimental trials designed to predict the microbial protein synthesis in the rumen via urinary purine derivatives require access to 24-h urinary volumes. This technique is non-invasive but requires access to 24-h urinary volumes, which is impracticable in grazing animals or a large number of feedlot sheep. The challenge is to provide the possibility of using the purine derivatives and creatinine concentrations in a spot urine sample and the daily creatinine excretion in urine as accurate estimators of purine derivatives’ excretion per day. ABSTRACT: The objective was to evaluate the influence of diets on lambs using different levels of peach palm meal as a replacement for maize (0, 10, 40, 60, and 85% of diet dry matter) on the endogenous creatinine clearance (CC), urine concentration ratio of purine derivatives to creatinine (PDC index), and daily creatinine excretion (DCE) as a marker to estimate purine derivatives (PD) excretion from urinary spot samples collected at different time points (4, 8, 12, 16, 20, 24 h after morning feeding) compared to 24-h total urine collection. The measured parameters were voluntary intake, urinary volume, CC, DCE, the concentration of plasma creatinine, and PD and purine derivatives’ excretion (PDE). Five lambs were allocated to metabolic cages and distributed in a 5 × 5 Latin square. Urine collection was taken daily on days 16 to 19 of each experimental period. The inclusion of peach palm meal linearly reduced the intake of dry matter (g kg BW(−0.75), p = 0.005), crude protein (g kg BW(−0.75), p = 0.010), metabolizable energy (MJ kg BW(−0.75), p = 0.010) and CC (p < 0.0001). It also quadratically affected the urinary volume (p = 0.008) and DCE (p = 0.004). There was a linear decrease for PDC index (p = 0.032) and PDE (p < 0.0001) measured in the 24-h total urine with peach palm meal levels. The different times of spot urine sampling did not affect (p > 0.05) the PDC index and PDE. Peach palm meal decreases the CC thereby compromising the use of a mean value of DCE as a PDE marker in spot urine samples. There is greater accuracy when using different values of DCE obtained for each diet as markers for the PDE in spot urine samples. Unconventional foodstuffs of low palatability affecting the voluntary intake of feed change the renal function. MDPI 2022-05-06 /pmc/articles/PMC9106062/ /pubmed/35565619 http://dx.doi.org/10.3390/ani12091195 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pereira, Taiala Cristina de Jesus
Pereira, Mara Lúcia Albuquerque
de Carvalho, Gleidson Giordano Pinto
Silva, Herymá Giovane de Oliveira
dos Santos, Alana Batista
Pina, Douglas dos Santos
Sousa, Leandro Borges
Creatinine as a Urinary Marker of the Purine Derivatives Excretion in Urine Spot Samples of Lambs Fed Peach Palm Meal
title Creatinine as a Urinary Marker of the Purine Derivatives Excretion in Urine Spot Samples of Lambs Fed Peach Palm Meal
title_full Creatinine as a Urinary Marker of the Purine Derivatives Excretion in Urine Spot Samples of Lambs Fed Peach Palm Meal
title_fullStr Creatinine as a Urinary Marker of the Purine Derivatives Excretion in Urine Spot Samples of Lambs Fed Peach Palm Meal
title_full_unstemmed Creatinine as a Urinary Marker of the Purine Derivatives Excretion in Urine Spot Samples of Lambs Fed Peach Palm Meal
title_short Creatinine as a Urinary Marker of the Purine Derivatives Excretion in Urine Spot Samples of Lambs Fed Peach Palm Meal
title_sort creatinine as a urinary marker of the purine derivatives excretion in urine spot samples of lambs fed peach palm meal
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106062/
https://www.ncbi.nlm.nih.gov/pubmed/35565619
http://dx.doi.org/10.3390/ani12091195
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