Cargando…
Lung cancer targeting efficiency of Silibinin loaded Poly Caprolactone /Pluronic F68 Inhalable nanoparticles: In vitro and In vivo study
Silibinin (SB) is shown to have an anticancer properties. However, its clinical therapeutic effects have been restricted due to its low water solubility and poor absorption after oral administration. The aim of this study was to develop SB-loaded PCL/Pluronic F68 nanoparticles for pulmonary delivery...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106168/ https://www.ncbi.nlm.nih.gov/pubmed/35560136 http://dx.doi.org/10.1371/journal.pone.0267257 |
_version_ | 1784708218510901248 |
---|---|
author | Patel, Priya Raval, Mihir Manvar, Aneka Airao, Vishal Bhatt, Vaibhav Shah, Pranav |
author_facet | Patel, Priya Raval, Mihir Manvar, Aneka Airao, Vishal Bhatt, Vaibhav Shah, Pranav |
author_sort | Patel, Priya |
collection | PubMed |
description | Silibinin (SB) is shown to have an anticancer properties. However, its clinical therapeutic effects have been restricted due to its low water solubility and poor absorption after oral administration. The aim of this study was to develop SB-loaded PCL/Pluronic F68 nanoparticles for pulmonary delivery in the treatment of lung cancer. A modified solvent displacement process was used to make nanoparticles, which were then lyophilized to make inhalation powder, Nanoparticles were characterized with DSC, FTIR,SEM and In vitro release study. Further, a validated HPLC method was developed to investigate the Biodistribution study, pharmacokinetic parameters. Poly Caprolactone PCL / Pluronic F68 NPs showed the sustained release effect up to 48 h with an emitted (Mass median Aerodynamic diameter)MMAD and (Geometric size distribution)GSD were found to be 4.235 ±0.124 and 1.958±1.23 respectively. More specifically, the SB Loaded PCL/Pluronic F 68 NPs demonstrated long circulation and successful lung tumor-targeting potential due to their cancer-targeting capabilities. SB Loaded PCL/Pluronic F68 NPs significantly inhibited tumour growth in lung cancer-induced rats after inhalable administration. In a pharmacokinetics study, PCL/ Pluronic F68 NPs substantially improved SB bioavailability, with a more than 4-fold rise in AUC when compared to IV administration. These findings indicate that SB-loaded PCL/PluronicF68 nanoparticles may be a successful lung cancer therapy delivery system. |
format | Online Article Text |
id | pubmed-9106168 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-91061682022-05-14 Lung cancer targeting efficiency of Silibinin loaded Poly Caprolactone /Pluronic F68 Inhalable nanoparticles: In vitro and In vivo study Patel, Priya Raval, Mihir Manvar, Aneka Airao, Vishal Bhatt, Vaibhav Shah, Pranav PLoS One Research Article Silibinin (SB) is shown to have an anticancer properties. However, its clinical therapeutic effects have been restricted due to its low water solubility and poor absorption after oral administration. The aim of this study was to develop SB-loaded PCL/Pluronic F68 nanoparticles for pulmonary delivery in the treatment of lung cancer. A modified solvent displacement process was used to make nanoparticles, which were then lyophilized to make inhalation powder, Nanoparticles were characterized with DSC, FTIR,SEM and In vitro release study. Further, a validated HPLC method was developed to investigate the Biodistribution study, pharmacokinetic parameters. Poly Caprolactone PCL / Pluronic F68 NPs showed the sustained release effect up to 48 h with an emitted (Mass median Aerodynamic diameter)MMAD and (Geometric size distribution)GSD were found to be 4.235 ±0.124 and 1.958±1.23 respectively. More specifically, the SB Loaded PCL/Pluronic F 68 NPs demonstrated long circulation and successful lung tumor-targeting potential due to their cancer-targeting capabilities. SB Loaded PCL/Pluronic F68 NPs significantly inhibited tumour growth in lung cancer-induced rats after inhalable administration. In a pharmacokinetics study, PCL/ Pluronic F68 NPs substantially improved SB bioavailability, with a more than 4-fold rise in AUC when compared to IV administration. These findings indicate that SB-loaded PCL/PluronicF68 nanoparticles may be a successful lung cancer therapy delivery system. Public Library of Science 2022-05-13 /pmc/articles/PMC9106168/ /pubmed/35560136 http://dx.doi.org/10.1371/journal.pone.0267257 Text en © 2022 Patel et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Patel, Priya Raval, Mihir Manvar, Aneka Airao, Vishal Bhatt, Vaibhav Shah, Pranav Lung cancer targeting efficiency of Silibinin loaded Poly Caprolactone /Pluronic F68 Inhalable nanoparticles: In vitro and In vivo study |
title | Lung cancer targeting efficiency of Silibinin loaded Poly Caprolactone /Pluronic F68 Inhalable nanoparticles: In vitro and In vivo study |
title_full | Lung cancer targeting efficiency of Silibinin loaded Poly Caprolactone /Pluronic F68 Inhalable nanoparticles: In vitro and In vivo study |
title_fullStr | Lung cancer targeting efficiency of Silibinin loaded Poly Caprolactone /Pluronic F68 Inhalable nanoparticles: In vitro and In vivo study |
title_full_unstemmed | Lung cancer targeting efficiency of Silibinin loaded Poly Caprolactone /Pluronic F68 Inhalable nanoparticles: In vitro and In vivo study |
title_short | Lung cancer targeting efficiency of Silibinin loaded Poly Caprolactone /Pluronic F68 Inhalable nanoparticles: In vitro and In vivo study |
title_sort | lung cancer targeting efficiency of silibinin loaded poly caprolactone /pluronic f68 inhalable nanoparticles: in vitro and in vivo study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106168/ https://www.ncbi.nlm.nih.gov/pubmed/35560136 http://dx.doi.org/10.1371/journal.pone.0267257 |
work_keys_str_mv | AT patelpriya lungcancertargetingefficiencyofsilibininloadedpolycaprolactonepluronicf68inhalablenanoparticlesinvitroandinvivostudy AT ravalmihir lungcancertargetingefficiencyofsilibininloadedpolycaprolactonepluronicf68inhalablenanoparticlesinvitroandinvivostudy AT manvaraneka lungcancertargetingefficiencyofsilibininloadedpolycaprolactonepluronicf68inhalablenanoparticlesinvitroandinvivostudy AT airaovishal lungcancertargetingefficiencyofsilibininloadedpolycaprolactonepluronicf68inhalablenanoparticlesinvitroandinvivostudy AT bhattvaibhav lungcancertargetingefficiencyofsilibininloadedpolycaprolactonepluronicf68inhalablenanoparticlesinvitroandinvivostudy AT shahpranav lungcancertargetingefficiencyofsilibininloadedpolycaprolactonepluronicf68inhalablenanoparticlesinvitroandinvivostudy |