Cargando…

Quantitative and qualitative evaluation of the hippocampal cytoarchitecture in adult cats with regard to the pathological diagnosis of hippocampal sclerosis

Cats are known to be affected by hippocampal sclerosis, potentially causing antiseizure drug(s) resistance. In order to lay the foundation for a standardized, systematic classification and diagnosis of this pathology in cats, this prospective study aimed at evaluating normal reference values of cell...

Descripción completa

Detalles Bibliográficos
Autores principales: Zilli, Jessica, Schänzer, Anne, Büttner, Kathrin, Kressin, Monika, Schmidt, Martin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106214/
https://www.ncbi.nlm.nih.gov/pubmed/35560321
http://dx.doi.org/10.1371/journal.pone.0268010
_version_ 1784708230326255616
author Zilli, Jessica
Schänzer, Anne
Büttner, Kathrin
Kressin, Monika
Schmidt, Martin J.
author_facet Zilli, Jessica
Schänzer, Anne
Büttner, Kathrin
Kressin, Monika
Schmidt, Martin J.
author_sort Zilli, Jessica
collection PubMed
description Cats are known to be affected by hippocampal sclerosis, potentially causing antiseizure drug(s) resistance. In order to lay the foundation for a standardized, systematic classification and diagnosis of this pathology in cats, this prospective study aimed at evaluating normal reference values of cellular densities and the cytoarchitecture of the feline hippocampus. Three transverse sections (head, body and tail) of each left hippocampus were obtained from 17 non-epileptic cats of different brachycephalic and mesocephalic breeds and age classes (range: 3–17 years). Histological (hematoxylin and eosin, Nissl) and immunohistochemical (NeuN, GFAP) staining was performed to investigate neuron and astroglial cell populations, as well as the layer thickness of the pyramidal cell layer and granule cell layer. Significant differences in neuronal density (in CA2-CA4 and the granule cell layer) and layer thickness (in CA1-CA3 and the granule cell layer) were evidenced throughout the longitudinal hippocampal axis (p<0.05); on the other hand, the astrocyte density did not differ. Moreover, reference ranges were defined for these parameters in the pyramidal cell layer and in the granule cell layer. The findings did not differ according to breed or age. In veterinary medicine these parameters have not been evaluated in cats so far. As surgical treatment may become a therapeutic option for cats with temporal lobe epilepsy, estimating normal values of the hippocampal cytoarchitecture will help in the standardized histopathological examination of resected hippocampal specimens to reach a diagnosis of hippocampal sclerosis.
format Online
Article
Text
id pubmed-9106214
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-91062142022-05-14 Quantitative and qualitative evaluation of the hippocampal cytoarchitecture in adult cats with regard to the pathological diagnosis of hippocampal sclerosis Zilli, Jessica Schänzer, Anne Büttner, Kathrin Kressin, Monika Schmidt, Martin J. PLoS One Research Article Cats are known to be affected by hippocampal sclerosis, potentially causing antiseizure drug(s) resistance. In order to lay the foundation for a standardized, systematic classification and diagnosis of this pathology in cats, this prospective study aimed at evaluating normal reference values of cellular densities and the cytoarchitecture of the feline hippocampus. Three transverse sections (head, body and tail) of each left hippocampus were obtained from 17 non-epileptic cats of different brachycephalic and mesocephalic breeds and age classes (range: 3–17 years). Histological (hematoxylin and eosin, Nissl) and immunohistochemical (NeuN, GFAP) staining was performed to investigate neuron and astroglial cell populations, as well as the layer thickness of the pyramidal cell layer and granule cell layer. Significant differences in neuronal density (in CA2-CA4 and the granule cell layer) and layer thickness (in CA1-CA3 and the granule cell layer) were evidenced throughout the longitudinal hippocampal axis (p<0.05); on the other hand, the astrocyte density did not differ. Moreover, reference ranges were defined for these parameters in the pyramidal cell layer and in the granule cell layer. The findings did not differ according to breed or age. In veterinary medicine these parameters have not been evaluated in cats so far. As surgical treatment may become a therapeutic option for cats with temporal lobe epilepsy, estimating normal values of the hippocampal cytoarchitecture will help in the standardized histopathological examination of resected hippocampal specimens to reach a diagnosis of hippocampal sclerosis. Public Library of Science 2022-05-13 /pmc/articles/PMC9106214/ /pubmed/35560321 http://dx.doi.org/10.1371/journal.pone.0268010 Text en © 2022 Zilli et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zilli, Jessica
Schänzer, Anne
Büttner, Kathrin
Kressin, Monika
Schmidt, Martin J.
Quantitative and qualitative evaluation of the hippocampal cytoarchitecture in adult cats with regard to the pathological diagnosis of hippocampal sclerosis
title Quantitative and qualitative evaluation of the hippocampal cytoarchitecture in adult cats with regard to the pathological diagnosis of hippocampal sclerosis
title_full Quantitative and qualitative evaluation of the hippocampal cytoarchitecture in adult cats with regard to the pathological diagnosis of hippocampal sclerosis
title_fullStr Quantitative and qualitative evaluation of the hippocampal cytoarchitecture in adult cats with regard to the pathological diagnosis of hippocampal sclerosis
title_full_unstemmed Quantitative and qualitative evaluation of the hippocampal cytoarchitecture in adult cats with regard to the pathological diagnosis of hippocampal sclerosis
title_short Quantitative and qualitative evaluation of the hippocampal cytoarchitecture in adult cats with regard to the pathological diagnosis of hippocampal sclerosis
title_sort quantitative and qualitative evaluation of the hippocampal cytoarchitecture in adult cats with regard to the pathological diagnosis of hippocampal sclerosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106214/
https://www.ncbi.nlm.nih.gov/pubmed/35560321
http://dx.doi.org/10.1371/journal.pone.0268010
work_keys_str_mv AT zillijessica quantitativeandqualitativeevaluationofthehippocampalcytoarchitectureinadultcatswithregardtothepathologicaldiagnosisofhippocampalsclerosis
AT schanzeranne quantitativeandqualitativeevaluationofthehippocampalcytoarchitectureinadultcatswithregardtothepathologicaldiagnosisofhippocampalsclerosis
AT buttnerkathrin quantitativeandqualitativeevaluationofthehippocampalcytoarchitectureinadultcatswithregardtothepathologicaldiagnosisofhippocampalsclerosis
AT kressinmonika quantitativeandqualitativeevaluationofthehippocampalcytoarchitectureinadultcatswithregardtothepathologicaldiagnosisofhippocampalsclerosis
AT schmidtmartinj quantitativeandqualitativeevaluationofthehippocampalcytoarchitectureinadultcatswithregardtothepathologicaldiagnosisofhippocampalsclerosis