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Urinary Biomarkers of Oxidative Stress in Aging: Implications for Prediction of Accelerated Biological Age in Prospective Cohort Studies

BACKGROUND: Aging is a major risk factor for a range of chronic diseases. Oxidative stress theory of aging has been previously proposed as one of the mechanisms responsible for the age-related decline in organ/tissue function and the development of age-related diseases. Urine contains rich biologica...

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Autores principales: Mukli, Peter, Wu, Dee H., Csipo, Tamas, Owens, Cameron D., Lipecz, Agnes, Racz, Frigyes Samuel, Zouein, Fouad A., Tabak, Adam, Csiszar, Anna, Ungvari, Zoltan, Tsitouras, Panayiotis D., Yabluchanskiy, Andriy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106456/
https://www.ncbi.nlm.nih.gov/pubmed/35571254
http://dx.doi.org/10.1155/2022/6110226
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author Mukli, Peter
Wu, Dee H.
Csipo, Tamas
Owens, Cameron D.
Lipecz, Agnes
Racz, Frigyes Samuel
Zouein, Fouad A.
Tabak, Adam
Csiszar, Anna
Ungvari, Zoltan
Tsitouras, Panayiotis D.
Yabluchanskiy, Andriy
author_facet Mukli, Peter
Wu, Dee H.
Csipo, Tamas
Owens, Cameron D.
Lipecz, Agnes
Racz, Frigyes Samuel
Zouein, Fouad A.
Tabak, Adam
Csiszar, Anna
Ungvari, Zoltan
Tsitouras, Panayiotis D.
Yabluchanskiy, Andriy
author_sort Mukli, Peter
collection PubMed
description BACKGROUND: Aging is a major risk factor for a range of chronic diseases. Oxidative stress theory of aging has been previously proposed as one of the mechanisms responsible for the age-related decline in organ/tissue function and the development of age-related diseases. Urine contains rich biological information on the health status of every major organ system and can be an important noninvasive source for biomarkers of systemic oxidative stress in aging. AIMS: The objective of this cross-sectional study was to validate a novel panel of urinary oxidative stress biomarkers. METHODS: Nucleic acid oxidation adducts and oxidative damage markers of lipids and proteins were assessed in urine samples from nondiabetic and currently nonsmoking subjects (n = 198) across different ages (20 to 89 years old). Urinary parameters and chronological age were correlated then the biological age of enrolled individuals was determined from the urinary oxidative stress markers using the algorithm of Klemera and Doubal. RESULTS: Our findings showed that 8-oxo-7,8-deoxyguanosine (8-oxoG), 8-oxo-7,8-dihydroguanosine (8-OHdG), and dityrosine (DTyr) positively correlated with chronological age, while the level of an F(2)-isoprostane (iPF(2)α-VI) correlated negatively with age. We found that 8-oxoG, DTyr, and iPF(2)α-VI were significantly higher among accelerated agers compared to nonaccelerated agers and that a decision tree model could successfully identify accelerated agers with an accuracy of >92%. Discussion. Our results indicate that 8-oxoG and iPF(2)α-VI levels in the urine reveal biological aging. CONCLUSION: Assessing urinary biomarkers of oxidative stress may be an important approach for the evaluation of biological age by identifying individuals at accelerated risk for the development of age-related diseases.
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spelling pubmed-91064562022-05-14 Urinary Biomarkers of Oxidative Stress in Aging: Implications for Prediction of Accelerated Biological Age in Prospective Cohort Studies Mukli, Peter Wu, Dee H. Csipo, Tamas Owens, Cameron D. Lipecz, Agnes Racz, Frigyes Samuel Zouein, Fouad A. Tabak, Adam Csiszar, Anna Ungvari, Zoltan Tsitouras, Panayiotis D. Yabluchanskiy, Andriy Oxid Med Cell Longev Research Article BACKGROUND: Aging is a major risk factor for a range of chronic diseases. Oxidative stress theory of aging has been previously proposed as one of the mechanisms responsible for the age-related decline in organ/tissue function and the development of age-related diseases. Urine contains rich biological information on the health status of every major organ system and can be an important noninvasive source for biomarkers of systemic oxidative stress in aging. AIMS: The objective of this cross-sectional study was to validate a novel panel of urinary oxidative stress biomarkers. METHODS: Nucleic acid oxidation adducts and oxidative damage markers of lipids and proteins were assessed in urine samples from nondiabetic and currently nonsmoking subjects (n = 198) across different ages (20 to 89 years old). Urinary parameters and chronological age were correlated then the biological age of enrolled individuals was determined from the urinary oxidative stress markers using the algorithm of Klemera and Doubal. RESULTS: Our findings showed that 8-oxo-7,8-deoxyguanosine (8-oxoG), 8-oxo-7,8-dihydroguanosine (8-OHdG), and dityrosine (DTyr) positively correlated with chronological age, while the level of an F(2)-isoprostane (iPF(2)α-VI) correlated negatively with age. We found that 8-oxoG, DTyr, and iPF(2)α-VI were significantly higher among accelerated agers compared to nonaccelerated agers and that a decision tree model could successfully identify accelerated agers with an accuracy of >92%. Discussion. Our results indicate that 8-oxoG and iPF(2)α-VI levels in the urine reveal biological aging. CONCLUSION: Assessing urinary biomarkers of oxidative stress may be an important approach for the evaluation of biological age by identifying individuals at accelerated risk for the development of age-related diseases. Hindawi 2022-05-06 /pmc/articles/PMC9106456/ /pubmed/35571254 http://dx.doi.org/10.1155/2022/6110226 Text en Copyright © 2022 Peter Mukli et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mukli, Peter
Wu, Dee H.
Csipo, Tamas
Owens, Cameron D.
Lipecz, Agnes
Racz, Frigyes Samuel
Zouein, Fouad A.
Tabak, Adam
Csiszar, Anna
Ungvari, Zoltan
Tsitouras, Panayiotis D.
Yabluchanskiy, Andriy
Urinary Biomarkers of Oxidative Stress in Aging: Implications for Prediction of Accelerated Biological Age in Prospective Cohort Studies
title Urinary Biomarkers of Oxidative Stress in Aging: Implications for Prediction of Accelerated Biological Age in Prospective Cohort Studies
title_full Urinary Biomarkers of Oxidative Stress in Aging: Implications for Prediction of Accelerated Biological Age in Prospective Cohort Studies
title_fullStr Urinary Biomarkers of Oxidative Stress in Aging: Implications for Prediction of Accelerated Biological Age in Prospective Cohort Studies
title_full_unstemmed Urinary Biomarkers of Oxidative Stress in Aging: Implications for Prediction of Accelerated Biological Age in Prospective Cohort Studies
title_short Urinary Biomarkers of Oxidative Stress in Aging: Implications for Prediction of Accelerated Biological Age in Prospective Cohort Studies
title_sort urinary biomarkers of oxidative stress in aging: implications for prediction of accelerated biological age in prospective cohort studies
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106456/
https://www.ncbi.nlm.nih.gov/pubmed/35571254
http://dx.doi.org/10.1155/2022/6110226
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