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Bioactive fish collagen peptides weaken intestinal inflammation by orienting colonic macrophages phenotype through mannose receptor activation

PURPOSE: Particular interest is now given to the potential of dietary supplements as alternative non-pharmacological approaches in intestinal inflammation handling. In this aim, this study evaluates the efficiency of fish collagen peptides, Naticol(®)Gut, on colonic inflammation. METHODS: Wild type...

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Autores principales: Rahabi, Mouna, Salon, Marie, Bruno-Bonnet, Christelle, Prat, Mélissa, Jacquemin, Godefroy, Benmoussa, Khaddouj, Alaeddine, Mohamad, Parny, Mélissa, Bernad, José, Bertrand, Bénédicte, Auffret, Yannick, Robert-Jolimaître, Pascale, Alric, Laurent, Authier, Hélène, Coste, Agnès
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106617/
https://www.ncbi.nlm.nih.gov/pubmed/34999930
http://dx.doi.org/10.1007/s00394-021-02787-7
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author Rahabi, Mouna
Salon, Marie
Bruno-Bonnet, Christelle
Prat, Mélissa
Jacquemin, Godefroy
Benmoussa, Khaddouj
Alaeddine, Mohamad
Parny, Mélissa
Bernad, José
Bertrand, Bénédicte
Auffret, Yannick
Robert-Jolimaître, Pascale
Alric, Laurent
Authier, Hélène
Coste, Agnès
author_facet Rahabi, Mouna
Salon, Marie
Bruno-Bonnet, Christelle
Prat, Mélissa
Jacquemin, Godefroy
Benmoussa, Khaddouj
Alaeddine, Mohamad
Parny, Mélissa
Bernad, José
Bertrand, Bénédicte
Auffret, Yannick
Robert-Jolimaître, Pascale
Alric, Laurent
Authier, Hélène
Coste, Agnès
author_sort Rahabi, Mouna
collection PubMed
description PURPOSE: Particular interest is now given to the potential of dietary supplements as alternative non-pharmacological approaches in intestinal inflammation handling. In this aim, this study evaluates the efficiency of fish collagen peptides, Naticol(®)Gut, on colonic inflammation. METHODS: Wild type and Mannose receptor-deficient in the myeloid lineage C57BL/6 mice were administered with Dextran Sodium Sulfate (DSS), Naticol(®)Gut, DSS, and Naticol(®)Gut or only water for 4 or 8 days. Inflammatory status was evaluated by establishing macroscopic and microscopic scores, by measuring cytokine and calprotectin production by ELISA and the myeloperoxidase activity by chemiluminescence. Colonic macrophages were phenotyped by measuring mRNA levels of specific markers of inflammation and oxidative status. Colonic immune populations and T-cell activation profiles were determined by flow cytometry. Mucosa-associated gut microbiota assessment was undertaken by qPCR. The phenotype of human blood monocytes from inflammatory bowel disease (IBD) subjects was characterized by RT-qPCR and flow cytometry and their oxidative activity by chemiluminescence. RESULTS: Naticol(®)Gut-treated DSS mice showed attenuated colonic inflammation compared to mice that were only exposed to DSS. Naticol(®)Gut activity was displayed through its ability to orient the polarization of colonic macrophage towards an anti-inflammatory and anti-oxidant phenotype after its recognition by the mannose receptor. Subsequently, Naticol(®)Gut delivery modulated CD4 T cells in favor of a Th2 response and dampened CD8 T-cell activation. This immunomodulation resulted in an intestinal eubiosis. In human monocytes from IBD subjects, the treatment with Naticol(®)Gut also restored an anti-inflammatory and anti-oxidant phenotype. CONCLUSION: Naticol(®)Gut acts as a protective agent against colitis appearing as a new functional food and an innovative and complementary approach in gut health. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00394-021-02787-7.
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spelling pubmed-91066172022-05-15 Bioactive fish collagen peptides weaken intestinal inflammation by orienting colonic macrophages phenotype through mannose receptor activation Rahabi, Mouna Salon, Marie Bruno-Bonnet, Christelle Prat, Mélissa Jacquemin, Godefroy Benmoussa, Khaddouj Alaeddine, Mohamad Parny, Mélissa Bernad, José Bertrand, Bénédicte Auffret, Yannick Robert-Jolimaître, Pascale Alric, Laurent Authier, Hélène Coste, Agnès Eur J Nutr Original Contribution PURPOSE: Particular interest is now given to the potential of dietary supplements as alternative non-pharmacological approaches in intestinal inflammation handling. In this aim, this study evaluates the efficiency of fish collagen peptides, Naticol(®)Gut, on colonic inflammation. METHODS: Wild type and Mannose receptor-deficient in the myeloid lineage C57BL/6 mice were administered with Dextran Sodium Sulfate (DSS), Naticol(®)Gut, DSS, and Naticol(®)Gut or only water for 4 or 8 days. Inflammatory status was evaluated by establishing macroscopic and microscopic scores, by measuring cytokine and calprotectin production by ELISA and the myeloperoxidase activity by chemiluminescence. Colonic macrophages were phenotyped by measuring mRNA levels of specific markers of inflammation and oxidative status. Colonic immune populations and T-cell activation profiles were determined by flow cytometry. Mucosa-associated gut microbiota assessment was undertaken by qPCR. The phenotype of human blood monocytes from inflammatory bowel disease (IBD) subjects was characterized by RT-qPCR and flow cytometry and their oxidative activity by chemiluminescence. RESULTS: Naticol(®)Gut-treated DSS mice showed attenuated colonic inflammation compared to mice that were only exposed to DSS. Naticol(®)Gut activity was displayed through its ability to orient the polarization of colonic macrophage towards an anti-inflammatory and anti-oxidant phenotype after its recognition by the mannose receptor. Subsequently, Naticol(®)Gut delivery modulated CD4 T cells in favor of a Th2 response and dampened CD8 T-cell activation. This immunomodulation resulted in an intestinal eubiosis. In human monocytes from IBD subjects, the treatment with Naticol(®)Gut also restored an anti-inflammatory and anti-oxidant phenotype. CONCLUSION: Naticol(®)Gut acts as a protective agent against colitis appearing as a new functional food and an innovative and complementary approach in gut health. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00394-021-02787-7. Springer Berlin Heidelberg 2022-01-08 2022 /pmc/articles/PMC9106617/ /pubmed/34999930 http://dx.doi.org/10.1007/s00394-021-02787-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Contribution
Rahabi, Mouna
Salon, Marie
Bruno-Bonnet, Christelle
Prat, Mélissa
Jacquemin, Godefroy
Benmoussa, Khaddouj
Alaeddine, Mohamad
Parny, Mélissa
Bernad, José
Bertrand, Bénédicte
Auffret, Yannick
Robert-Jolimaître, Pascale
Alric, Laurent
Authier, Hélène
Coste, Agnès
Bioactive fish collagen peptides weaken intestinal inflammation by orienting colonic macrophages phenotype through mannose receptor activation
title Bioactive fish collagen peptides weaken intestinal inflammation by orienting colonic macrophages phenotype through mannose receptor activation
title_full Bioactive fish collagen peptides weaken intestinal inflammation by orienting colonic macrophages phenotype through mannose receptor activation
title_fullStr Bioactive fish collagen peptides weaken intestinal inflammation by orienting colonic macrophages phenotype through mannose receptor activation
title_full_unstemmed Bioactive fish collagen peptides weaken intestinal inflammation by orienting colonic macrophages phenotype through mannose receptor activation
title_short Bioactive fish collagen peptides weaken intestinal inflammation by orienting colonic macrophages phenotype through mannose receptor activation
title_sort bioactive fish collagen peptides weaken intestinal inflammation by orienting colonic macrophages phenotype through mannose receptor activation
topic Original Contribution
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106617/
https://www.ncbi.nlm.nih.gov/pubmed/34999930
http://dx.doi.org/10.1007/s00394-021-02787-7
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