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On the origins of conductive pulse sensing inside a nanopore

Nanopore sensing is nearly synonymous with resistive pulse sensing due to the characteristic occlusion of ions during pore occupancy, particularly at high salt concentrations. Contrarily, conductive pulses are observed under low salt conditions wherein electroosmotic flow is significant. Most litera...

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Autores principales: Lastra, Lauren S., Bandara, Y. M. Nuwan D. Y., Nguyen, Michelle, Farajpour, Nasim, Freedman, Kevin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106702/
https://www.ncbi.nlm.nih.gov/pubmed/35562332
http://dx.doi.org/10.1038/s41467-022-29758-8
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author Lastra, Lauren S.
Bandara, Y. M. Nuwan D. Y.
Nguyen, Michelle
Farajpour, Nasim
Freedman, Kevin J.
author_facet Lastra, Lauren S.
Bandara, Y. M. Nuwan D. Y.
Nguyen, Michelle
Farajpour, Nasim
Freedman, Kevin J.
author_sort Lastra, Lauren S.
collection PubMed
description Nanopore sensing is nearly synonymous with resistive pulse sensing due to the characteristic occlusion of ions during pore occupancy, particularly at high salt concentrations. Contrarily, conductive pulses are observed under low salt conditions wherein electroosmotic flow is significant. Most literature reports counterions as the dominant mechanism of conductive events (a molecule-centric theory). However, the counterion theory does not fit well with conductive events occurring via net neutral-charged protein translocation, prompting further investigation into translocation mechanics. Herein, we demonstrate theory and experiments underpinning the translocation mechanism (i.e., electroosmosis or electrophoresis), pulse direction (i.e., conductive or resistive) and shape (e.g., monophasic or biphasic) through fine control of chemical, physical, and electronic parameters. Results from these studies predict strong electroosmosis plays a role in driving DNA events and generating conductive events due to polarization effects (i.e., a pore-centric theory).
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spelling pubmed-91067022022-05-15 On the origins of conductive pulse sensing inside a nanopore Lastra, Lauren S. Bandara, Y. M. Nuwan D. Y. Nguyen, Michelle Farajpour, Nasim Freedman, Kevin J. Nat Commun Article Nanopore sensing is nearly synonymous with resistive pulse sensing due to the characteristic occlusion of ions during pore occupancy, particularly at high salt concentrations. Contrarily, conductive pulses are observed under low salt conditions wherein electroosmotic flow is significant. Most literature reports counterions as the dominant mechanism of conductive events (a molecule-centric theory). However, the counterion theory does not fit well with conductive events occurring via net neutral-charged protein translocation, prompting further investigation into translocation mechanics. Herein, we demonstrate theory and experiments underpinning the translocation mechanism (i.e., electroosmosis or electrophoresis), pulse direction (i.e., conductive or resistive) and shape (e.g., monophasic or biphasic) through fine control of chemical, physical, and electronic parameters. Results from these studies predict strong electroosmosis plays a role in driving DNA events and generating conductive events due to polarization effects (i.e., a pore-centric theory). Nature Publishing Group UK 2022-05-13 /pmc/articles/PMC9106702/ /pubmed/35562332 http://dx.doi.org/10.1038/s41467-022-29758-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Lastra, Lauren S.
Bandara, Y. M. Nuwan D. Y.
Nguyen, Michelle
Farajpour, Nasim
Freedman, Kevin J.
On the origins of conductive pulse sensing inside a nanopore
title On the origins of conductive pulse sensing inside a nanopore
title_full On the origins of conductive pulse sensing inside a nanopore
title_fullStr On the origins of conductive pulse sensing inside a nanopore
title_full_unstemmed On the origins of conductive pulse sensing inside a nanopore
title_short On the origins of conductive pulse sensing inside a nanopore
title_sort on the origins of conductive pulse sensing inside a nanopore
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106702/
https://www.ncbi.nlm.nih.gov/pubmed/35562332
http://dx.doi.org/10.1038/s41467-022-29758-8
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