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Direct RNA targeted in situ sequencing for transcriptomic profiling in tissue

Highly multiplexed spatial mapping of transcripts within tissues allows for investigation of the transcriptomic and cellular diversity of mammalian organs previously unseen. Here we explore a direct RNA (dRNA) detection approach incorporating the use of padlock probes and rolling circle amplificatio...

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Detalles Bibliográficos
Autores principales:	Lee, Hower, Marco Salas, Sergio, Gyllborg, Daniel, Nilsson, Mats
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106737/ https://www.ncbi.nlm.nih.gov/pubmed/35562352 http://dx.doi.org/10.1038/s41598-022-11534-9

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