Identification and genome characterization of novel parechovirus sequences from Hipposideros armiger in China

BACKGROUND: Bats were identified as a natural reservoir of emerging and re-emerging infectious pathogens threatening human health and life. METHODS: This study collected 21 fecal samples of Hipposideros armiger in Mengla County of Xishuangbanna Prefecture Yunnan Province to combine one pool for vira...

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Autores principales: Zeng, Jian, Yang, Zijun, Guo, Wentao, Wang, Xiaochun, Yang, Shixing, Shen, Quan, Wang, Hao, Zhang, Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107582/
https://www.ncbi.nlm.nih.gov/pubmed/35570277
http://dx.doi.org/10.1186/s12985-022-01806-1
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author Zeng, Jian
Yang, Zijun
Guo, Wentao
Wang, Xiaochun
Yang, Shixing
Shen, Quan
Wang, Hao
Zhang, Wen
author_facet Zeng, Jian
Yang, Zijun
Guo, Wentao
Wang, Xiaochun
Yang, Shixing
Shen, Quan
Wang, Hao
Zhang, Wen
author_sort Zeng, Jian
collection PubMed
description BACKGROUND: Bats were identified as a natural reservoir of emerging and re-emerging infectious pathogens threatening human health and life. METHODS: This study collected 21 fecal samples of Hipposideros armiger in Mengla County of Xishuangbanna Prefecture Yunnan Province to combine one pool for viral metagenomic sequencing. RESULTS: Two nearly complete genomes of parechoviruses, BPeV11 and BPeV20, were sequenced. Genome analysis revealed that BPeV11 and BPeV20 follow a 3-3-4 genome layout: 5′ UTR-VP0-VP3-VP1-2A-2B-2C-3A-3B-3C-3D-3′ UTR. The prevalence of BPev11 and BPev20 by Nested-PCR showed that 1 of 21 fecal samples was positive. Based on amino acid identity comparison and phylogenetic analysis of P1, 2C, and 3D, BPeV11 and BPeV20 were closely related to but distinct from FPeVs. CONCLUSION: It was probably proposed to be a novel species in the genus Parechovirus of the family Picornaviridae. The isolation of BPev11 and BPev20 from H. armiger in China is the first complete genome of parechovirus isolations from bat feces of the genus Hipposideros. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12985-022-01806-1.
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spelling pubmed-91075822022-05-16 Identification and genome characterization of novel parechovirus sequences from Hipposideros armiger in China Zeng, Jian Yang, Zijun Guo, Wentao Wang, Xiaochun Yang, Shixing Shen, Quan Wang, Hao Zhang, Wen Virol J Research BACKGROUND: Bats were identified as a natural reservoir of emerging and re-emerging infectious pathogens threatening human health and life. METHODS: This study collected 21 fecal samples of Hipposideros armiger in Mengla County of Xishuangbanna Prefecture Yunnan Province to combine one pool for viral metagenomic sequencing. RESULTS: Two nearly complete genomes of parechoviruses, BPeV11 and BPeV20, were sequenced. Genome analysis revealed that BPeV11 and BPeV20 follow a 3-3-4 genome layout: 5′ UTR-VP0-VP3-VP1-2A-2B-2C-3A-3B-3C-3D-3′ UTR. The prevalence of BPev11 and BPev20 by Nested-PCR showed that 1 of 21 fecal samples was positive. Based on amino acid identity comparison and phylogenetic analysis of P1, 2C, and 3D, BPeV11 and BPeV20 were closely related to but distinct from FPeVs. CONCLUSION: It was probably proposed to be a novel species in the genus Parechovirus of the family Picornaviridae. The isolation of BPev11 and BPev20 from H. armiger in China is the first complete genome of parechovirus isolations from bat feces of the genus Hipposideros. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12985-022-01806-1. BioMed Central 2022-05-15 /pmc/articles/PMC9107582/ /pubmed/35570277 http://dx.doi.org/10.1186/s12985-022-01806-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Zeng, Jian
Yang, Zijun
Guo, Wentao
Wang, Xiaochun
Yang, Shixing
Shen, Quan
Wang, Hao
Zhang, Wen
Identification and genome characterization of novel parechovirus sequences from Hipposideros armiger in China
title Identification and genome characterization of novel parechovirus sequences from Hipposideros armiger in China
title_full Identification and genome characterization of novel parechovirus sequences from Hipposideros armiger in China
title_fullStr Identification and genome characterization of novel parechovirus sequences from Hipposideros armiger in China
title_full_unstemmed Identification and genome characterization of novel parechovirus sequences from Hipposideros armiger in China
title_short Identification and genome characterization of novel parechovirus sequences from Hipposideros armiger in China
title_sort identification and genome characterization of novel parechovirus sequences from hipposideros armiger in china
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107582/
https://www.ncbi.nlm.nih.gov/pubmed/35570277
http://dx.doi.org/10.1186/s12985-022-01806-1
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