CRISPR-Cas12–based detection of SARS-CoV-2

An outbreak of betacoronavirus SARS-CoV-2 began in Wuhan, China in December 2019. COVID-19, the disease associated with infection, rapidly spread to produce a global pandemic. We report development of a rapid (<40 min), easy-to-implement and accurate CRISPR-Cas12-based lateral flow assay for dete...

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Autores principales: Broughton, James P., Deng, Xianding, Yu, Guixia, Fasching, Clare L., Servellita, Venice, Singh, Jasmeet, Miao, Xin, Streithorst, Jessica, Granados, Andrea, Sotomayor-Gonzalez, Alicia, Zorn, Kelsey, Gopez, Allan, Hsu, Elaine, Gu, Wei, Miller, Steve, Pan, Chao-Yang, Guevara, Hugo, Wadford, Debra A., Chen, Janice S., Chiu, Charles Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107629/
https://www.ncbi.nlm.nih.gov/pubmed/32300245
http://dx.doi.org/10.1038/s41587-020-0513-4
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author Broughton, James P.
Deng, Xianding
Yu, Guixia
Fasching, Clare L.
Servellita, Venice
Singh, Jasmeet
Miao, Xin
Streithorst, Jessica
Granados, Andrea
Sotomayor-Gonzalez, Alicia
Zorn, Kelsey
Gopez, Allan
Hsu, Elaine
Gu, Wei
Miller, Steve
Pan, Chao-Yang
Guevara, Hugo
Wadford, Debra A.
Chen, Janice S.
Chiu, Charles Y.
author_facet Broughton, James P.
Deng, Xianding
Yu, Guixia
Fasching, Clare L.
Servellita, Venice
Singh, Jasmeet
Miao, Xin
Streithorst, Jessica
Granados, Andrea
Sotomayor-Gonzalez, Alicia
Zorn, Kelsey
Gopez, Allan
Hsu, Elaine
Gu, Wei
Miller, Steve
Pan, Chao-Yang
Guevara, Hugo
Wadford, Debra A.
Chen, Janice S.
Chiu, Charles Y.
author_sort Broughton, James P.
collection PubMed
description An outbreak of betacoronavirus SARS-CoV-2 began in Wuhan, China in December 2019. COVID-19, the disease associated with infection, rapidly spread to produce a global pandemic. We report development of a rapid (<40 min), easy-to-implement and accurate CRISPR-Cas12-based lateral flow assay for detection of SARS-CoV-2 from respiratory swab RNA extracts. We validated our method using contrived reference samples and clinical samples from US patients, including 36 patients with COVID-19 infection and 42 patients with other viral respiratory infections. Our CRISPR-based DETECTR assay provides a visual and faster alternative to the US CDC SARS-CoV-2 real-time RT-PCR assay, with 95% positive predictive agreement and 100% negative predictive agreement..
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spelling pubmed-91076292022-05-15 CRISPR-Cas12–based detection of SARS-CoV-2 Broughton, James P. Deng, Xianding Yu, Guixia Fasching, Clare L. Servellita, Venice Singh, Jasmeet Miao, Xin Streithorst, Jessica Granados, Andrea Sotomayor-Gonzalez, Alicia Zorn, Kelsey Gopez, Allan Hsu, Elaine Gu, Wei Miller, Steve Pan, Chao-Yang Guevara, Hugo Wadford, Debra A. Chen, Janice S. Chiu, Charles Y. Nat Biotechnol Article An outbreak of betacoronavirus SARS-CoV-2 began in Wuhan, China in December 2019. COVID-19, the disease associated with infection, rapidly spread to produce a global pandemic. We report development of a rapid (<40 min), easy-to-implement and accurate CRISPR-Cas12-based lateral flow assay for detection of SARS-CoV-2 from respiratory swab RNA extracts. We validated our method using contrived reference samples and clinical samples from US patients, including 36 patients with COVID-19 infection and 42 patients with other viral respiratory infections. Our CRISPR-based DETECTR assay provides a visual and faster alternative to the US CDC SARS-CoV-2 real-time RT-PCR assay, with 95% positive predictive agreement and 100% negative predictive agreement.. 2020-07 2020-04-16 /pmc/articles/PMC9107629/ /pubmed/32300245 http://dx.doi.org/10.1038/s41587-020-0513-4 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Broughton, James P.
Deng, Xianding
Yu, Guixia
Fasching, Clare L.
Servellita, Venice
Singh, Jasmeet
Miao, Xin
Streithorst, Jessica
Granados, Andrea
Sotomayor-Gonzalez, Alicia
Zorn, Kelsey
Gopez, Allan
Hsu, Elaine
Gu, Wei
Miller, Steve
Pan, Chao-Yang
Guevara, Hugo
Wadford, Debra A.
Chen, Janice S.
Chiu, Charles Y.
CRISPR-Cas12–based detection of SARS-CoV-2
title CRISPR-Cas12–based detection of SARS-CoV-2
title_full CRISPR-Cas12–based detection of SARS-CoV-2
title_fullStr CRISPR-Cas12–based detection of SARS-CoV-2
title_full_unstemmed CRISPR-Cas12–based detection of SARS-CoV-2
title_short CRISPR-Cas12–based detection of SARS-CoV-2
title_sort crispr-cas12–based detection of sars-cov-2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107629/
https://www.ncbi.nlm.nih.gov/pubmed/32300245
http://dx.doi.org/10.1038/s41587-020-0513-4
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