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Biosynthesis of eriodictyol from tyrosine by Corynebacterium glutamicum

BACKGROUND: Eriodictyol is a bioactive flavonoid compound that shows potential applications in medicine development and food processing. Microbial synthesis of eriodictyol has been attracting increasing attention due to several benefits. In this study, we employed a GRAS strain Corynebacterium gluta...

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Autores principales: Wu, Xia, Liu, Jingyi, Liu, Dan, Yuwen, Miaomiao, Koffas, Mattheos A. G., Zha, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107716/
https://www.ncbi.nlm.nih.gov/pubmed/35568867
http://dx.doi.org/10.1186/s12934-022-01815-3
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author Wu, Xia
Liu, Jingyi
Liu, Dan
Yuwen, Miaomiao
Koffas, Mattheos A. G.
Zha, Jian
author_facet Wu, Xia
Liu, Jingyi
Liu, Dan
Yuwen, Miaomiao
Koffas, Mattheos A. G.
Zha, Jian
author_sort Wu, Xia
collection PubMed
description BACKGROUND: Eriodictyol is a bioactive flavonoid compound that shows potential applications in medicine development and food processing. Microbial synthesis of eriodictyol has been attracting increasing attention due to several benefits. In this study, we employed a GRAS strain Corynebacterium glutamicum as the host to produce eriodictyol directly from tyrosine. RESULTS: We firstly optimized the biosynthetic module of naringenin, the upstream intermediate for eriodictyol production, through screening of different gene orthologues. Next, to improve the level of the precursor malonyl-CoA necessary for naringenin production, we introduced matB and matC from Rhizobium trifolii into C. glutamicum to convert extracellular malonate to intracellular malonyl-CoA. This combinatorial engineering resulted in around 35-fold increase in naringenin production from tyrosine compared to the initial recombinant C. glutamicum. Subsequently, the hpaBC genes from E. coli encoding 4-hydroxyphenylacetate 3-hydroxylase were expressed in C. glutamicum to synthesize eriodictyol from naringenin. Further optimization of the biotransformation process parameters led to the production of 14.10 mg/L eriodictyol. CONCLUSIONS: The biosynthesis of the ortho-hydroxylated flavonoid eriodictyol in C. glutamicum was achieved for the first time via functional expression of E. coli hpaBC, providing a baseline strain for biosynthesis of other complex flavonoids. Our study demonstrates the potential application of C. glutamicum as a host microbe for the biosynthesis of value-added natural compounds from tyrosine.
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spelling pubmed-91077162022-05-16 Biosynthesis of eriodictyol from tyrosine by Corynebacterium glutamicum Wu, Xia Liu, Jingyi Liu, Dan Yuwen, Miaomiao Koffas, Mattheos A. G. Zha, Jian Microb Cell Fact Research BACKGROUND: Eriodictyol is a bioactive flavonoid compound that shows potential applications in medicine development and food processing. Microbial synthesis of eriodictyol has been attracting increasing attention due to several benefits. In this study, we employed a GRAS strain Corynebacterium glutamicum as the host to produce eriodictyol directly from tyrosine. RESULTS: We firstly optimized the biosynthetic module of naringenin, the upstream intermediate for eriodictyol production, through screening of different gene orthologues. Next, to improve the level of the precursor malonyl-CoA necessary for naringenin production, we introduced matB and matC from Rhizobium trifolii into C. glutamicum to convert extracellular malonate to intracellular malonyl-CoA. This combinatorial engineering resulted in around 35-fold increase in naringenin production from tyrosine compared to the initial recombinant C. glutamicum. Subsequently, the hpaBC genes from E. coli encoding 4-hydroxyphenylacetate 3-hydroxylase were expressed in C. glutamicum to synthesize eriodictyol from naringenin. Further optimization of the biotransformation process parameters led to the production of 14.10 mg/L eriodictyol. CONCLUSIONS: The biosynthesis of the ortho-hydroxylated flavonoid eriodictyol in C. glutamicum was achieved for the first time via functional expression of E. coli hpaBC, providing a baseline strain for biosynthesis of other complex flavonoids. Our study demonstrates the potential application of C. glutamicum as a host microbe for the biosynthesis of value-added natural compounds from tyrosine. BioMed Central 2022-05-14 /pmc/articles/PMC9107716/ /pubmed/35568867 http://dx.doi.org/10.1186/s12934-022-01815-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Wu, Xia
Liu, Jingyi
Liu, Dan
Yuwen, Miaomiao
Koffas, Mattheos A. G.
Zha, Jian
Biosynthesis of eriodictyol from tyrosine by Corynebacterium glutamicum
title Biosynthesis of eriodictyol from tyrosine by Corynebacterium glutamicum
title_full Biosynthesis of eriodictyol from tyrosine by Corynebacterium glutamicum
title_fullStr Biosynthesis of eriodictyol from tyrosine by Corynebacterium glutamicum
title_full_unstemmed Biosynthesis of eriodictyol from tyrosine by Corynebacterium glutamicum
title_short Biosynthesis of eriodictyol from tyrosine by Corynebacterium glutamicum
title_sort biosynthesis of eriodictyol from tyrosine by corynebacterium glutamicum
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107716/
https://www.ncbi.nlm.nih.gov/pubmed/35568867
http://dx.doi.org/10.1186/s12934-022-01815-3
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