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Comprehensive analysis of circRNA expression profiles in rat cerebral cortex after moderate traumatic brain injury
Traumatic brain injury is a medical event of global concern, and a growing body of research suggests that circular RNAs can play very important roles in traumatic brain injury. To explore the functions of more novel and valuable circular RNA in traumatic brain injury response, a moderate traumatic b...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9108397/ https://www.ncbi.nlm.nih.gov/pubmed/35582420 http://dx.doi.org/10.7150/ijms.71769 |
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author | Li, Gang Li, Shaoping Liu, Ruining Yu, Jiangtao Ma, Haoli Zhao, Yan |
author_facet | Li, Gang Li, Shaoping Liu, Ruining Yu, Jiangtao Ma, Haoli Zhao, Yan |
author_sort | Li, Gang |
collection | PubMed |
description | Traumatic brain injury is a medical event of global concern, and a growing body of research suggests that circular RNAs can play very important roles in traumatic brain injury. To explore the functions of more novel and valuable circular RNA in traumatic brain injury response, a moderate traumatic brain injury in rats was established and comprehensive analysis of circular RNA expression profiles in rat cerebral cortex was done. As a result, 301 up-regulated and 284 down-regulated circular RNAs were obtained in moderate traumatic brain injury rats, the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis were performed based on the circular RNA's host genes, and a circRNA-miRNA interaction network based on differentially expressed circular RNAs was constructed. Also, four circular RNAs were validated by RT-qPCR and Sanger sequencing. This study showed that differentially expressed circular RNAs existed between rat cerebral cortex after moderate traumatic brain injury and control. And this will provide valuable information for circular RNA research in the field of traumatic brain injury. |
format | Online Article Text |
id | pubmed-9108397 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-91083972022-05-16 Comprehensive analysis of circRNA expression profiles in rat cerebral cortex after moderate traumatic brain injury Li, Gang Li, Shaoping Liu, Ruining Yu, Jiangtao Ma, Haoli Zhao, Yan Int J Med Sci Research Paper Traumatic brain injury is a medical event of global concern, and a growing body of research suggests that circular RNAs can play very important roles in traumatic brain injury. To explore the functions of more novel and valuable circular RNA in traumatic brain injury response, a moderate traumatic brain injury in rats was established and comprehensive analysis of circular RNA expression profiles in rat cerebral cortex was done. As a result, 301 up-regulated and 284 down-regulated circular RNAs were obtained in moderate traumatic brain injury rats, the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis were performed based on the circular RNA's host genes, and a circRNA-miRNA interaction network based on differentially expressed circular RNAs was constructed. Also, four circular RNAs were validated by RT-qPCR and Sanger sequencing. This study showed that differentially expressed circular RNAs existed between rat cerebral cortex after moderate traumatic brain injury and control. And this will provide valuable information for circular RNA research in the field of traumatic brain injury. Ivyspring International Publisher 2022-04-18 /pmc/articles/PMC9108397/ /pubmed/35582420 http://dx.doi.org/10.7150/ijms.71769 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Li, Gang Li, Shaoping Liu, Ruining Yu, Jiangtao Ma, Haoli Zhao, Yan Comprehensive analysis of circRNA expression profiles in rat cerebral cortex after moderate traumatic brain injury |
title | Comprehensive analysis of circRNA expression profiles in rat cerebral cortex after moderate traumatic brain injury |
title_full | Comprehensive analysis of circRNA expression profiles in rat cerebral cortex after moderate traumatic brain injury |
title_fullStr | Comprehensive analysis of circRNA expression profiles in rat cerebral cortex after moderate traumatic brain injury |
title_full_unstemmed | Comprehensive analysis of circRNA expression profiles in rat cerebral cortex after moderate traumatic brain injury |
title_short | Comprehensive analysis of circRNA expression profiles in rat cerebral cortex after moderate traumatic brain injury |
title_sort | comprehensive analysis of circrna expression profiles in rat cerebral cortex after moderate traumatic brain injury |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9108397/ https://www.ncbi.nlm.nih.gov/pubmed/35582420 http://dx.doi.org/10.7150/ijms.71769 |
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