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Characterization and microsatellite marker development for a common bark and ambrosia beetle associate, Geosmithia obscura

Symbioses between Geosmithia fungi and wood‐boring and bark beetles seldom result in disease induction within the plant host. Yet, exceptions exist such as Geosmithia morbida, the causal agent of Thousand Cankers Disease (TCD) of walnuts and wingnuts, and Geosmithia sp. 41, the causal agent of Foamy...

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Autores principales: Pietsch, Grace M., Gazis, Romina, Klingeman, William E., Huff, Matthew L., Staton, Margaret E., Kolarik, Miroslav, Hadziabdic, Denita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9108439/
https://www.ncbi.nlm.nih.gov/pubmed/35765178
http://dx.doi.org/10.1002/mbo3.1286
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author Pietsch, Grace M.
Gazis, Romina
Klingeman, William E.
Huff, Matthew L.
Staton, Margaret E.
Kolarik, Miroslav
Hadziabdic, Denita
author_facet Pietsch, Grace M.
Gazis, Romina
Klingeman, William E.
Huff, Matthew L.
Staton, Margaret E.
Kolarik, Miroslav
Hadziabdic, Denita
author_sort Pietsch, Grace M.
collection PubMed
description Symbioses between Geosmithia fungi and wood‐boring and bark beetles seldom result in disease induction within the plant host. Yet, exceptions exist such as Geosmithia morbida, the causal agent of Thousand Cankers Disease (TCD) of walnuts and wingnuts, and Geosmithia sp. 41, the causal agent of Foamy Bark Canker disease of oaks. Isolates of G. obscura were recovered from black walnut trees in eastern Tennessee and at least one isolate induced cankers following artificial inoculation. Due to the putative pathogenicity and lack of recovery of G. obscura from natural lesions, a molecular diagnostic screening tool was developed using microsatellite markers mined from the G. obscura genome. A total of 3256 candidate microsatellite markers were identified (2236, 789, 137 di‐, tri‐, and tetranucleotide motifs, respectively), with 2011, 703, 101 di‐, tri‐, and tetranucleotide motifs, respectively, containing markers with primers. From these, 75 microsatellite markers were randomly selected, screened, and optimized, resulting in 28 polymorphic markers that yielded single, consistently recovered bands, which were used in downstream analyses. Five of these microsatellite markers were found to be specific to G. obscura and did not cross‐amplify into other, closely related species. Although the remaining tested markers could be useful, they cross‐amplified within different Geosmithia species, making them not reliable for G. obscura detection. Five novel microsatellite markers (GOBS9, GOBS10, GOBS41, GOBS43, and GOBS50) were developed based on the G. obscura genome. These species‐specific microsatellite markers are available as a tool for use in molecular diagnostics and can assist future surveillance studies.
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spelling pubmed-91084392022-05-20 Characterization and microsatellite marker development for a common bark and ambrosia beetle associate, Geosmithia obscura Pietsch, Grace M. Gazis, Romina Klingeman, William E. Huff, Matthew L. Staton, Margaret E. Kolarik, Miroslav Hadziabdic, Denita Microbiologyopen Original Articles Symbioses between Geosmithia fungi and wood‐boring and bark beetles seldom result in disease induction within the plant host. Yet, exceptions exist such as Geosmithia morbida, the causal agent of Thousand Cankers Disease (TCD) of walnuts and wingnuts, and Geosmithia sp. 41, the causal agent of Foamy Bark Canker disease of oaks. Isolates of G. obscura were recovered from black walnut trees in eastern Tennessee and at least one isolate induced cankers following artificial inoculation. Due to the putative pathogenicity and lack of recovery of G. obscura from natural lesions, a molecular diagnostic screening tool was developed using microsatellite markers mined from the G. obscura genome. A total of 3256 candidate microsatellite markers were identified (2236, 789, 137 di‐, tri‐, and tetranucleotide motifs, respectively), with 2011, 703, 101 di‐, tri‐, and tetranucleotide motifs, respectively, containing markers with primers. From these, 75 microsatellite markers were randomly selected, screened, and optimized, resulting in 28 polymorphic markers that yielded single, consistently recovered bands, which were used in downstream analyses. Five of these microsatellite markers were found to be specific to G. obscura and did not cross‐amplify into other, closely related species. Although the remaining tested markers could be useful, they cross‐amplified within different Geosmithia species, making them not reliable for G. obscura detection. Five novel microsatellite markers (GOBS9, GOBS10, GOBS41, GOBS43, and GOBS50) were developed based on the G. obscura genome. These species‐specific microsatellite markers are available as a tool for use in molecular diagnostics and can assist future surveillance studies. John Wiley and Sons Inc. 2022-05-15 /pmc/articles/PMC9108439/ /pubmed/35765178 http://dx.doi.org/10.1002/mbo3.1286 Text en © 2022 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Pietsch, Grace M.
Gazis, Romina
Klingeman, William E.
Huff, Matthew L.
Staton, Margaret E.
Kolarik, Miroslav
Hadziabdic, Denita
Characterization and microsatellite marker development for a common bark and ambrosia beetle associate, Geosmithia obscura
title Characterization and microsatellite marker development for a common bark and ambrosia beetle associate, Geosmithia obscura
title_full Characterization and microsatellite marker development for a common bark and ambrosia beetle associate, Geosmithia obscura
title_fullStr Characterization and microsatellite marker development for a common bark and ambrosia beetle associate, Geosmithia obscura
title_full_unstemmed Characterization and microsatellite marker development for a common bark and ambrosia beetle associate, Geosmithia obscura
title_short Characterization and microsatellite marker development for a common bark and ambrosia beetle associate, Geosmithia obscura
title_sort characterization and microsatellite marker development for a common bark and ambrosia beetle associate, geosmithia obscura
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9108439/
https://www.ncbi.nlm.nih.gov/pubmed/35765178
http://dx.doi.org/10.1002/mbo3.1286
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