Injection of human neuroblastoma cells into neural crest streams in live zebrafish embryos

Cancer cell behavior is highly microenvironment dependent, but we have a limited understanding of malignant cell-microenvironment interactions in vivo. Here, we describe a protocol for xenotransplanting human neuroblastoma (NB) cells into streams of migrating neural crest stem cells in zebrafish emb...

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Detalles Bibliográficos
Autores principales: Ibarra, Beatriz A., Jiang, Xinghang, Treffy, Randall W., Saxena, Ankur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9108679/
https://www.ncbi.nlm.nih.gov/pubmed/35586316
http://dx.doi.org/10.1016/j.xpro.2022.101380
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author Ibarra, Beatriz A.
Jiang, Xinghang
Treffy, Randall W.
Saxena, Ankur
author_facet Ibarra, Beatriz A.
Jiang, Xinghang
Treffy, Randall W.
Saxena, Ankur
author_sort Ibarra, Beatriz A.
collection PubMed
description Cancer cell behavior is highly microenvironment dependent, but we have a limited understanding of malignant cell-microenvironment interactions in vivo. Here, we describe a protocol for xenotransplanting human neuroblastoma (NB) cells into streams of migrating neural crest stem cells in zebrafish embryos, followed by confocal time-lapse imaging and cell tracking. This high-resolution model system facilitates the quantitative spatiotemporal analysis of cancer cell-cell and cell-environment interactions. For complete details on the use and execution of this protocol, please refer to Treffy et al. (2021).
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spelling pubmed-91086792022-05-17 Injection of human neuroblastoma cells into neural crest streams in live zebrafish embryos Ibarra, Beatriz A. Jiang, Xinghang Treffy, Randall W. Saxena, Ankur STAR Protoc Protocol Cancer cell behavior is highly microenvironment dependent, but we have a limited understanding of malignant cell-microenvironment interactions in vivo. Here, we describe a protocol for xenotransplanting human neuroblastoma (NB) cells into streams of migrating neural crest stem cells in zebrafish embryos, followed by confocal time-lapse imaging and cell tracking. This high-resolution model system facilitates the quantitative spatiotemporal analysis of cancer cell-cell and cell-environment interactions. For complete details on the use and execution of this protocol, please refer to Treffy et al. (2021). Elsevier 2022-05-09 /pmc/articles/PMC9108679/ /pubmed/35586316 http://dx.doi.org/10.1016/j.xpro.2022.101380 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ibarra, Beatriz A.
Jiang, Xinghang
Treffy, Randall W.
Saxena, Ankur
Injection of human neuroblastoma cells into neural crest streams in live zebrafish embryos
title Injection of human neuroblastoma cells into neural crest streams in live zebrafish embryos
title_full Injection of human neuroblastoma cells into neural crest streams in live zebrafish embryos
title_fullStr Injection of human neuroblastoma cells into neural crest streams in live zebrafish embryos
title_full_unstemmed Injection of human neuroblastoma cells into neural crest streams in live zebrafish embryos
title_short Injection of human neuroblastoma cells into neural crest streams in live zebrafish embryos
title_sort injection of human neuroblastoma cells into neural crest streams in live zebrafish embryos
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9108679/
https://www.ncbi.nlm.nih.gov/pubmed/35586316
http://dx.doi.org/10.1016/j.xpro.2022.101380
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