Cargando…
Regulation of Neural Differentiation of ADMSCs using Graphene‐Mediated Wireless‐Localized Electrical Signals Driven by Electromagnetic Induction
Although adipose‐derived mesenchymal stem cells (ADMSCs) isolated from patients’ fat are considered as the most important autologous stem cells for tissue repair, significant difficulties in the neural differentiation of ADMSCs still impede stem cell therapy for neurodegenerative diseases. Herein, a...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9109060/ https://www.ncbi.nlm.nih.gov/pubmed/35152569 http://dx.doi.org/10.1002/advs.202104424 |
_version_ | 1784708832411254784 |
---|---|
author | Guo, Zhijie Sun, Chunhui Yang, Hongru Gao, Haoyang Liang, Na Wang, Jian Hu, Shuang Ren, Na Pang, Jinbo Wang, Jingang Meng, Ning Han, Lin Liu, Hong |
author_facet | Guo, Zhijie Sun, Chunhui Yang, Hongru Gao, Haoyang Liang, Na Wang, Jian Hu, Shuang Ren, Na Pang, Jinbo Wang, Jingang Meng, Ning Han, Lin Liu, Hong |
author_sort | Guo, Zhijie |
collection | PubMed |
description | Although adipose‐derived mesenchymal stem cells (ADMSCs) isolated from patients’ fat are considered as the most important autologous stem cells for tissue repair, significant difficulties in the neural differentiation of ADMSCs still impede stem cell therapy for neurodegenerative diseases. Herein, a wireless‐electrical stimulation method is proposed to direct the neural differentiation of ADMSCs based on the electromagnetic effect using a graphene film as a conductive scaffold. By placing a rotating magnet on the top of a culture system without any inducer, the ADMSCs cultured on graphene differentiate into functional neurons within 15 days. As a conductive biodegradable nanomaterial, graphene film acts as a wireless electrical signal generator driven by the electromagnetic induction, and millivolt‐level voltage generated in situ provokes ADMSCs to differentiate into neurons, proved by morphological variation, extremely high levels of neuron‐specific genes, and proteins. Most importantly, Ca(2+) intracellular influx is observed in these ADMSC‐derived neurons once exposure to neurotransmitters, indicating that these cells are functional neurons. This research enhances stem cell therapy for neurodegenerative diseases using autologous ADMSCs and overcomes the lack of neural stem cells. This nanostructure‐mediated physical‐signal simulation method is inexpensive, safe, and localized, and has a significant impact on neural regeneration. |
format | Online Article Text |
id | pubmed-9109060 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91090602022-05-20 Regulation of Neural Differentiation of ADMSCs using Graphene‐Mediated Wireless‐Localized Electrical Signals Driven by Electromagnetic Induction Guo, Zhijie Sun, Chunhui Yang, Hongru Gao, Haoyang Liang, Na Wang, Jian Hu, Shuang Ren, Na Pang, Jinbo Wang, Jingang Meng, Ning Han, Lin Liu, Hong Adv Sci (Weinh) Research Articles Although adipose‐derived mesenchymal stem cells (ADMSCs) isolated from patients’ fat are considered as the most important autologous stem cells for tissue repair, significant difficulties in the neural differentiation of ADMSCs still impede stem cell therapy for neurodegenerative diseases. Herein, a wireless‐electrical stimulation method is proposed to direct the neural differentiation of ADMSCs based on the electromagnetic effect using a graphene film as a conductive scaffold. By placing a rotating magnet on the top of a culture system without any inducer, the ADMSCs cultured on graphene differentiate into functional neurons within 15 days. As a conductive biodegradable nanomaterial, graphene film acts as a wireless electrical signal generator driven by the electromagnetic induction, and millivolt‐level voltage generated in situ provokes ADMSCs to differentiate into neurons, proved by morphological variation, extremely high levels of neuron‐specific genes, and proteins. Most importantly, Ca(2+) intracellular influx is observed in these ADMSC‐derived neurons once exposure to neurotransmitters, indicating that these cells are functional neurons. This research enhances stem cell therapy for neurodegenerative diseases using autologous ADMSCs and overcomes the lack of neural stem cells. This nanostructure‐mediated physical‐signal simulation method is inexpensive, safe, and localized, and has a significant impact on neural regeneration. John Wiley and Sons Inc. 2022-02-12 /pmc/articles/PMC9109060/ /pubmed/35152569 http://dx.doi.org/10.1002/advs.202104424 Text en © 2022 The Authors. Advanced Science published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Guo, Zhijie Sun, Chunhui Yang, Hongru Gao, Haoyang Liang, Na Wang, Jian Hu, Shuang Ren, Na Pang, Jinbo Wang, Jingang Meng, Ning Han, Lin Liu, Hong Regulation of Neural Differentiation of ADMSCs using Graphene‐Mediated Wireless‐Localized Electrical Signals Driven by Electromagnetic Induction |
title | Regulation of Neural Differentiation of ADMSCs using Graphene‐Mediated Wireless‐Localized Electrical Signals Driven by Electromagnetic Induction |
title_full | Regulation of Neural Differentiation of ADMSCs using Graphene‐Mediated Wireless‐Localized Electrical Signals Driven by Electromagnetic Induction |
title_fullStr | Regulation of Neural Differentiation of ADMSCs using Graphene‐Mediated Wireless‐Localized Electrical Signals Driven by Electromagnetic Induction |
title_full_unstemmed | Regulation of Neural Differentiation of ADMSCs using Graphene‐Mediated Wireless‐Localized Electrical Signals Driven by Electromagnetic Induction |
title_short | Regulation of Neural Differentiation of ADMSCs using Graphene‐Mediated Wireless‐Localized Electrical Signals Driven by Electromagnetic Induction |
title_sort | regulation of neural differentiation of admscs using graphene‐mediated wireless‐localized electrical signals driven by electromagnetic induction |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9109060/ https://www.ncbi.nlm.nih.gov/pubmed/35152569 http://dx.doi.org/10.1002/advs.202104424 |
work_keys_str_mv | AT guozhijie regulationofneuraldifferentiationofadmscsusinggraphenemediatedwirelesslocalizedelectricalsignalsdrivenbyelectromagneticinduction AT sunchunhui regulationofneuraldifferentiationofadmscsusinggraphenemediatedwirelesslocalizedelectricalsignalsdrivenbyelectromagneticinduction AT yanghongru regulationofneuraldifferentiationofadmscsusinggraphenemediatedwirelesslocalizedelectricalsignalsdrivenbyelectromagneticinduction AT gaohaoyang regulationofneuraldifferentiationofadmscsusinggraphenemediatedwirelesslocalizedelectricalsignalsdrivenbyelectromagneticinduction AT liangna regulationofneuraldifferentiationofadmscsusinggraphenemediatedwirelesslocalizedelectricalsignalsdrivenbyelectromagneticinduction AT wangjian regulationofneuraldifferentiationofadmscsusinggraphenemediatedwirelesslocalizedelectricalsignalsdrivenbyelectromagneticinduction AT hushuang regulationofneuraldifferentiationofadmscsusinggraphenemediatedwirelesslocalizedelectricalsignalsdrivenbyelectromagneticinduction AT renna regulationofneuraldifferentiationofadmscsusinggraphenemediatedwirelesslocalizedelectricalsignalsdrivenbyelectromagneticinduction AT pangjinbo regulationofneuraldifferentiationofadmscsusinggraphenemediatedwirelesslocalizedelectricalsignalsdrivenbyelectromagneticinduction AT wangjingang regulationofneuraldifferentiationofadmscsusinggraphenemediatedwirelesslocalizedelectricalsignalsdrivenbyelectromagneticinduction AT mengning regulationofneuraldifferentiationofadmscsusinggraphenemediatedwirelesslocalizedelectricalsignalsdrivenbyelectromagneticinduction AT hanlin regulationofneuraldifferentiationofadmscsusinggraphenemediatedwirelesslocalizedelectricalsignalsdrivenbyelectromagneticinduction AT liuhong regulationofneuraldifferentiationofadmscsusinggraphenemediatedwirelesslocalizedelectricalsignalsdrivenbyelectromagneticinduction |