Cargando…
Bellidifolin Inhibits SRY-Related High Mobility Group-Box Gene 9 to Block TGF-β Signalling Activation to Ameliorate Myocardial Fibrosis
Myocardial fibrosis is the main morphological change of ventricular remodelling caused by cardiovascular diseases, mainly manifested due to the excessive production of collagen proteins. SRY-related high mobility group-box gene 9 (SOX9) is a new target regulating myocardial fibrosis. Bellidifolin (B...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110156/ https://www.ncbi.nlm.nih.gov/pubmed/35586685 http://dx.doi.org/10.1155/2022/6841276 |
_version_ | 1784709038472167424 |
---|---|
author | Yao, Ting-Ting Yang, Hong-Xia Sun, Jia-Huan Zhang, Yue Zhang, Yu Song, Qiu-Hang Liu, Wei-Zhe Zhang, Juan-Juan Li, Ai-Ying |
author_facet | Yao, Ting-Ting Yang, Hong-Xia Sun, Jia-Huan Zhang, Yue Zhang, Yu Song, Qiu-Hang Liu, Wei-Zhe Zhang, Juan-Juan Li, Ai-Ying |
author_sort | Yao, Ting-Ting |
collection | PubMed |
description | Myocardial fibrosis is the main morphological change of ventricular remodelling caused by cardiovascular diseases, mainly manifested due to the excessive production of collagen proteins. SRY-related high mobility group-box gene 9 (SOX9) is a new target regulating myocardial fibrosis. Bellidifolin (BEL), the active component of G. acuta, can prevent heart damage. However, it is unclear whether BEL can regulate SOX9 to alleviate myocardial fibrosis. The mice were subjected to isoproterenol (ISO) to establish myocardial fibrosis, and human myocardial fibroblasts (HCFs) were activated by TGF-β1 in the present study. The pathological changes of cardiac tissue were observed by HE staining. Masson staining was applied to reveal the collagen deposition in the heart. The measurement for expression of fibrosis-related proteins, SOX9, and TGF-β1 signalling molecules adopted Western blot and immunohistochemistry. The effects of BEL on HCFs, activity were detected by CCK-8. The result showed that BEL did not affect cell viability. And, the data indicated that BEL inhibited the elevations in α-SMA, Collagen I, and Collagen III by decreasing SOX9 expression. Additionally, SOX9 suppression by siRNA downregulated the TGF-β1 expression and prevented Smad3 phosphorylation, as supported by reducing the expression of α-SMA, Collagen I, and Collagen III. In vivo study verified that BEL ameliorated myocardial fibrosis by inhibiting SOX9. Therefore, BEL inhibited SOX9 to block TGF-β1 signalling activation to ameliorate myocardial fibrosis. |
format | Online Article Text |
id | pubmed-9110156 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-91101562022-05-17 Bellidifolin Inhibits SRY-Related High Mobility Group-Box Gene 9 to Block TGF-β Signalling Activation to Ameliorate Myocardial Fibrosis Yao, Ting-Ting Yang, Hong-Xia Sun, Jia-Huan Zhang, Yue Zhang, Yu Song, Qiu-Hang Liu, Wei-Zhe Zhang, Juan-Juan Li, Ai-Ying Evid Based Complement Alternat Med Research Article Myocardial fibrosis is the main morphological change of ventricular remodelling caused by cardiovascular diseases, mainly manifested due to the excessive production of collagen proteins. SRY-related high mobility group-box gene 9 (SOX9) is a new target regulating myocardial fibrosis. Bellidifolin (BEL), the active component of G. acuta, can prevent heart damage. However, it is unclear whether BEL can regulate SOX9 to alleviate myocardial fibrosis. The mice were subjected to isoproterenol (ISO) to establish myocardial fibrosis, and human myocardial fibroblasts (HCFs) were activated by TGF-β1 in the present study. The pathological changes of cardiac tissue were observed by HE staining. Masson staining was applied to reveal the collagen deposition in the heart. The measurement for expression of fibrosis-related proteins, SOX9, and TGF-β1 signalling molecules adopted Western blot and immunohistochemistry. The effects of BEL on HCFs, activity were detected by CCK-8. The result showed that BEL did not affect cell viability. And, the data indicated that BEL inhibited the elevations in α-SMA, Collagen I, and Collagen III by decreasing SOX9 expression. Additionally, SOX9 suppression by siRNA downregulated the TGF-β1 expression and prevented Smad3 phosphorylation, as supported by reducing the expression of α-SMA, Collagen I, and Collagen III. In vivo study verified that BEL ameliorated myocardial fibrosis by inhibiting SOX9. Therefore, BEL inhibited SOX9 to block TGF-β1 signalling activation to ameliorate myocardial fibrosis. Hindawi 2022-05-09 /pmc/articles/PMC9110156/ /pubmed/35586685 http://dx.doi.org/10.1155/2022/6841276 Text en Copyright © 2022 Ting-Ting Yao et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yao, Ting-Ting Yang, Hong-Xia Sun, Jia-Huan Zhang, Yue Zhang, Yu Song, Qiu-Hang Liu, Wei-Zhe Zhang, Juan-Juan Li, Ai-Ying Bellidifolin Inhibits SRY-Related High Mobility Group-Box Gene 9 to Block TGF-β Signalling Activation to Ameliorate Myocardial Fibrosis |
title | Bellidifolin Inhibits SRY-Related High Mobility Group-Box Gene 9 to Block TGF-β Signalling Activation to Ameliorate Myocardial Fibrosis |
title_full | Bellidifolin Inhibits SRY-Related High Mobility Group-Box Gene 9 to Block TGF-β Signalling Activation to Ameliorate Myocardial Fibrosis |
title_fullStr | Bellidifolin Inhibits SRY-Related High Mobility Group-Box Gene 9 to Block TGF-β Signalling Activation to Ameliorate Myocardial Fibrosis |
title_full_unstemmed | Bellidifolin Inhibits SRY-Related High Mobility Group-Box Gene 9 to Block TGF-β Signalling Activation to Ameliorate Myocardial Fibrosis |
title_short | Bellidifolin Inhibits SRY-Related High Mobility Group-Box Gene 9 to Block TGF-β Signalling Activation to Ameliorate Myocardial Fibrosis |
title_sort | bellidifolin inhibits sry-related high mobility group-box gene 9 to block tgf-β signalling activation to ameliorate myocardial fibrosis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110156/ https://www.ncbi.nlm.nih.gov/pubmed/35586685 http://dx.doi.org/10.1155/2022/6841276 |
work_keys_str_mv | AT yaotingting bellidifolininhibitssryrelatedhighmobilitygroupboxgene9toblocktgfbsignallingactivationtoamelioratemyocardialfibrosis AT yanghongxia bellidifolininhibitssryrelatedhighmobilitygroupboxgene9toblocktgfbsignallingactivationtoamelioratemyocardialfibrosis AT sunjiahuan bellidifolininhibitssryrelatedhighmobilitygroupboxgene9toblocktgfbsignallingactivationtoamelioratemyocardialfibrosis AT zhangyue bellidifolininhibitssryrelatedhighmobilitygroupboxgene9toblocktgfbsignallingactivationtoamelioratemyocardialfibrosis AT zhangyu bellidifolininhibitssryrelatedhighmobilitygroupboxgene9toblocktgfbsignallingactivationtoamelioratemyocardialfibrosis AT songqiuhang bellidifolininhibitssryrelatedhighmobilitygroupboxgene9toblocktgfbsignallingactivationtoamelioratemyocardialfibrosis AT liuweizhe bellidifolininhibitssryrelatedhighmobilitygroupboxgene9toblocktgfbsignallingactivationtoamelioratemyocardialfibrosis AT zhangjuanjuan bellidifolininhibitssryrelatedhighmobilitygroupboxgene9toblocktgfbsignallingactivationtoamelioratemyocardialfibrosis AT liaiying bellidifolininhibitssryrelatedhighmobilitygroupboxgene9toblocktgfbsignallingactivationtoamelioratemyocardialfibrosis |