Transcriptomic profile of the predatory mite Amblyseius swirskii (Acari: Phytoseiidae) on different host plants

Amblyseius swirskii Athias-Henriot (Acari: Phytoseiidae) is a predatory mite, effective at controlling whiteflies and thrips in protected crops. However, on tomato its efficacy as a biocontrol agent is hindered, most probably by the plant trichomes and their exudates. Our aim was to characterize the response of A. swirskii to the tomato trichome exudates and identify three major detoxification gene sets in this species: cytochromes P450 (CYPs), glutathione S-transferases (GSTs) and carboxyl/cholinesterases (CCEs). Mites were exposed separately to tomato and pepper, a favourable host plant for A. swirskii, after which their transcriptional responses were analysed and compared. The de novo transcriptome assembly resulted in 71,336 unigenes with 66.1% of them annotated. Thirty-nine A. swirskii genes were differentially expressed after transfer on tomato leaves when compared to pepper leaves; some of the expressed genes were associated with the metabolism of tomato exudates. Our results illustrate that the detoxification gene sets CYPs, GSTs and CCEs are abundant in A. swirskii, but do not play a significant role when in contact with the tomato exudates. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10493-022-00715-w.