Novel electron microscopic staining method using traditional dye, hematoxylin

Uranyl acetate (UA) has been routinely used as a staining solution for ultrathin sections used in biological electron microscopy. As a radioactive nuclear material, UA is subject to strict international regulations. To develop an alternative and easy-to-use staining method for ultrathin sections, we...

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Autores principales: Sasaki, Hiroyuki, Arai, Hisako, Kikuchi, Emi, Saito, Hideki, Seki, Keiko, Matsui, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110702/
https://www.ncbi.nlm.nih.gov/pubmed/35577902
http://dx.doi.org/10.1038/s41598-022-11523-y
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author Sasaki, Hiroyuki
Arai, Hisako
Kikuchi, Emi
Saito, Hideki
Seki, Keiko
Matsui, Takeshi
author_facet Sasaki, Hiroyuki
Arai, Hisako
Kikuchi, Emi
Saito, Hideki
Seki, Keiko
Matsui, Takeshi
author_sort Sasaki, Hiroyuki
collection PubMed
description Uranyl acetate (UA) has been routinely used as a staining solution for ultrathin sections used in biological electron microscopy. As a radioactive nuclear material, UA is subject to strict international regulations. To develop an alternative and easy-to-use staining method for ultrathin sections, we examined various commercial light microscopic dyes. We found that Mayer’s hematoxylin followed by Reynold’s lead citrate solution showed staining results comparable to UA and Reynold’s lead citrate solution, and this method is therefore suggested as a reliable and promising alternative to UA staining.
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spelling pubmed-91107022022-05-18 Novel electron microscopic staining method using traditional dye, hematoxylin Sasaki, Hiroyuki Arai, Hisako Kikuchi, Emi Saito, Hideki Seki, Keiko Matsui, Takeshi Sci Rep Article Uranyl acetate (UA) has been routinely used as a staining solution for ultrathin sections used in biological electron microscopy. As a radioactive nuclear material, UA is subject to strict international regulations. To develop an alternative and easy-to-use staining method for ultrathin sections, we examined various commercial light microscopic dyes. We found that Mayer’s hematoxylin followed by Reynold’s lead citrate solution showed staining results comparable to UA and Reynold’s lead citrate solution, and this method is therefore suggested as a reliable and promising alternative to UA staining. Nature Publishing Group UK 2022-05-16 /pmc/articles/PMC9110702/ /pubmed/35577902 http://dx.doi.org/10.1038/s41598-022-11523-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Sasaki, Hiroyuki
Arai, Hisako
Kikuchi, Emi
Saito, Hideki
Seki, Keiko
Matsui, Takeshi
Novel electron microscopic staining method using traditional dye, hematoxylin
title Novel electron microscopic staining method using traditional dye, hematoxylin
title_full Novel electron microscopic staining method using traditional dye, hematoxylin
title_fullStr Novel electron microscopic staining method using traditional dye, hematoxylin
title_full_unstemmed Novel electron microscopic staining method using traditional dye, hematoxylin
title_short Novel electron microscopic staining method using traditional dye, hematoxylin
title_sort novel electron microscopic staining method using traditional dye, hematoxylin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110702/
https://www.ncbi.nlm.nih.gov/pubmed/35577902
http://dx.doi.org/10.1038/s41598-022-11523-y
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