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Comparative Analysis of the Morphological, Physiological, Proteomic, and Metabolic Mechanisms of the “Biloxi” Blueberry Response to Shade Stress

Blueberry is an important small berry crop in economic forests. In hot summers, the top tip of blueberry often burns and withers due to water loss. Therefore, this study subjected blueberry to shading treatment in the summer to study the effects of different shading treatments on the growth, morphol...

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Autores principales: Wu, Yaqiong, Huang, Zhengjin, Zhang, Chunhong, Shi, Chong, Lyu, Lianfei, Li, Weilin, Wu, Wenlong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9111170/
https://www.ncbi.nlm.nih.gov/pubmed/35592566
http://dx.doi.org/10.3389/fpls.2022.877789
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author Wu, Yaqiong
Huang, Zhengjin
Zhang, Chunhong
Shi, Chong
Lyu, Lianfei
Li, Weilin
Wu, Wenlong
author_facet Wu, Yaqiong
Huang, Zhengjin
Zhang, Chunhong
Shi, Chong
Lyu, Lianfei
Li, Weilin
Wu, Wenlong
author_sort Wu, Yaqiong
collection PubMed
description Blueberry is an important small berry crop in economic forests. In hot summers, the top tip of blueberry often burns and withers due to water loss. Therefore, this study subjected blueberry to shading treatment in the summer to study the effects of different shading treatments on the growth, morphology, physiology and protein levels of the plant. The results showed that the 50% shading (T1) treatment yielded the highest average increases in plant height, crown width, and ground diameter of blueberry. Under the 80% shading (T2) treatment, the cells of the leaves dissolved, the morphology was incomplete, the vascular bundles disappeared, and no supporting skeleton was detected. As demonstrated by physiological and biochemical data and the proteome expression levels, the T1 shading treatment was beneficial to the growth of blueberry and significantly enriched the photosynthetic pathway and flavonoid biosynthesis. An analysis of the interaction network of differentially expressed proteins indicated that trans-cinnamate 4-monooxygenase (C4H, CYP73A), naringenin 3-dioxygenase (F3H) and bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase (DFR) exhibited high connectivity and mutual regulation. In short, 50% shading can improve the growth index of blueberry and lead to an enrichment of flavonoid biosynthesis. This study provides a scientific basis for the breeding and summer protection of blueberry seedlings.
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spelling pubmed-91111702022-05-18 Comparative Analysis of the Morphological, Physiological, Proteomic, and Metabolic Mechanisms of the “Biloxi” Blueberry Response to Shade Stress Wu, Yaqiong Huang, Zhengjin Zhang, Chunhong Shi, Chong Lyu, Lianfei Li, Weilin Wu, Wenlong Front Plant Sci Plant Science Blueberry is an important small berry crop in economic forests. In hot summers, the top tip of blueberry often burns and withers due to water loss. Therefore, this study subjected blueberry to shading treatment in the summer to study the effects of different shading treatments on the growth, morphology, physiology and protein levels of the plant. The results showed that the 50% shading (T1) treatment yielded the highest average increases in plant height, crown width, and ground diameter of blueberry. Under the 80% shading (T2) treatment, the cells of the leaves dissolved, the morphology was incomplete, the vascular bundles disappeared, and no supporting skeleton was detected. As demonstrated by physiological and biochemical data and the proteome expression levels, the T1 shading treatment was beneficial to the growth of blueberry and significantly enriched the photosynthetic pathway and flavonoid biosynthesis. An analysis of the interaction network of differentially expressed proteins indicated that trans-cinnamate 4-monooxygenase (C4H, CYP73A), naringenin 3-dioxygenase (F3H) and bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase (DFR) exhibited high connectivity and mutual regulation. In short, 50% shading can improve the growth index of blueberry and lead to an enrichment of flavonoid biosynthesis. This study provides a scientific basis for the breeding and summer protection of blueberry seedlings. Frontiers Media S.A. 2022-05-03 /pmc/articles/PMC9111170/ /pubmed/35592566 http://dx.doi.org/10.3389/fpls.2022.877789 Text en Copyright © 2022 Wu, Huang, Zhang, Shi, Lyu, Li and Wu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Wu, Yaqiong
Huang, Zhengjin
Zhang, Chunhong
Shi, Chong
Lyu, Lianfei
Li, Weilin
Wu, Wenlong
Comparative Analysis of the Morphological, Physiological, Proteomic, and Metabolic Mechanisms of the “Biloxi” Blueberry Response to Shade Stress
title Comparative Analysis of the Morphological, Physiological, Proteomic, and Metabolic Mechanisms of the “Biloxi” Blueberry Response to Shade Stress
title_full Comparative Analysis of the Morphological, Physiological, Proteomic, and Metabolic Mechanisms of the “Biloxi” Blueberry Response to Shade Stress
title_fullStr Comparative Analysis of the Morphological, Physiological, Proteomic, and Metabolic Mechanisms of the “Biloxi” Blueberry Response to Shade Stress
title_full_unstemmed Comparative Analysis of the Morphological, Physiological, Proteomic, and Metabolic Mechanisms of the “Biloxi” Blueberry Response to Shade Stress
title_short Comparative Analysis of the Morphological, Physiological, Proteomic, and Metabolic Mechanisms of the “Biloxi” Blueberry Response to Shade Stress
title_sort comparative analysis of the morphological, physiological, proteomic, and metabolic mechanisms of the “biloxi” blueberry response to shade stress
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9111170/
https://www.ncbi.nlm.nih.gov/pubmed/35592566
http://dx.doi.org/10.3389/fpls.2022.877789
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