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New Insights Into the Local Auxin Biosynthesis and Its Effects on the Rapid Growth of Moso Bamboo (Phyllostachys edulis)

Auxin plays a crucial regulatory role in higher plants, but systematic studies on the location of auxin local biosynthesis are rare in bamboo and other graminaceous plants. We studied moso bamboo (Phyllostachys edulis), which can grow up to 1 m/day and serves as a reference species for bamboo and ot...

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Autores principales: Bai, Yucong, Cai, Miaomiao, Mu, Changhong, Cheng, Wenlong, Zheng, Huifang, Cheng, Zhanchao, Li, Juan, Mu, Shaohua, Gao, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9111533/
https://www.ncbi.nlm.nih.gov/pubmed/35592571
http://dx.doi.org/10.3389/fpls.2022.858686
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author Bai, Yucong
Cai, Miaomiao
Mu, Changhong
Cheng, Wenlong
Zheng, Huifang
Cheng, Zhanchao
Li, Juan
Mu, Shaohua
Gao, Jian
author_facet Bai, Yucong
Cai, Miaomiao
Mu, Changhong
Cheng, Wenlong
Zheng, Huifang
Cheng, Zhanchao
Li, Juan
Mu, Shaohua
Gao, Jian
author_sort Bai, Yucong
collection PubMed
description Auxin plays a crucial regulatory role in higher plants, but systematic studies on the location of auxin local biosynthesis are rare in bamboo and other graminaceous plants. We studied moso bamboo (Phyllostachys edulis), which can grow up to 1 m/day and serves as a reference species for bamboo and other fast-growing species. We selected young tissues such as root tips, shoot tips, young culm sheaths, sheath blades, and internode divisions for local auxin biosynthesis site analysis. IAA immunofluorescence localization revealed that auxin was similarly distributed in different stages of 50-cm and 300-cm bamboo shoots. Shoot tips had the highest auxin content, and it may be the main site of auxin biosynthesis in the early stage of rapid growth. A total of 22 key genes in the YUCCA family for auxin biosynthesis were identified by genome-wide identification, and these had obvious tissue-specific and spatio-temporal expression patterns. In situ hybridization analysis revealed that the localization of YUCCA genes was highly consistent with the distribution of auxin. Six major auxin synthesis genes, PheYUC3-1, PheYUC6-1, PheYUC6-3, PheYUC9-1, PheYUC9-2, and PheYUC7-3, were obtained that may have regulatory roles in auxin accumulation during moso bamboo growth. Culm sheaths were found to serve as the main local sites of auxin biosynthesis and the auxin required for internode elongation may be achieved mainly by auxin transport.
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spelling pubmed-91115332022-05-18 New Insights Into the Local Auxin Biosynthesis and Its Effects on the Rapid Growth of Moso Bamboo (Phyllostachys edulis) Bai, Yucong Cai, Miaomiao Mu, Changhong Cheng, Wenlong Zheng, Huifang Cheng, Zhanchao Li, Juan Mu, Shaohua Gao, Jian Front Plant Sci Plant Science Auxin plays a crucial regulatory role in higher plants, but systematic studies on the location of auxin local biosynthesis are rare in bamboo and other graminaceous plants. We studied moso bamboo (Phyllostachys edulis), which can grow up to 1 m/day and serves as a reference species for bamboo and other fast-growing species. We selected young tissues such as root tips, shoot tips, young culm sheaths, sheath blades, and internode divisions for local auxin biosynthesis site analysis. IAA immunofluorescence localization revealed that auxin was similarly distributed in different stages of 50-cm and 300-cm bamboo shoots. Shoot tips had the highest auxin content, and it may be the main site of auxin biosynthesis in the early stage of rapid growth. A total of 22 key genes in the YUCCA family for auxin biosynthesis were identified by genome-wide identification, and these had obvious tissue-specific and spatio-temporal expression patterns. In situ hybridization analysis revealed that the localization of YUCCA genes was highly consistent with the distribution of auxin. Six major auxin synthesis genes, PheYUC3-1, PheYUC6-1, PheYUC6-3, PheYUC9-1, PheYUC9-2, and PheYUC7-3, were obtained that may have regulatory roles in auxin accumulation during moso bamboo growth. Culm sheaths were found to serve as the main local sites of auxin biosynthesis and the auxin required for internode elongation may be achieved mainly by auxin transport. Frontiers Media S.A. 2022-05-03 /pmc/articles/PMC9111533/ /pubmed/35592571 http://dx.doi.org/10.3389/fpls.2022.858686 Text en Copyright © 2022 Bai, Cai, Mu, Cheng, Zheng, Cheng, Li, Mu and Gao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Bai, Yucong
Cai, Miaomiao
Mu, Changhong
Cheng, Wenlong
Zheng, Huifang
Cheng, Zhanchao
Li, Juan
Mu, Shaohua
Gao, Jian
New Insights Into the Local Auxin Biosynthesis and Its Effects on the Rapid Growth of Moso Bamboo (Phyllostachys edulis)
title New Insights Into the Local Auxin Biosynthesis and Its Effects on the Rapid Growth of Moso Bamboo (Phyllostachys edulis)
title_full New Insights Into the Local Auxin Biosynthesis and Its Effects on the Rapid Growth of Moso Bamboo (Phyllostachys edulis)
title_fullStr New Insights Into the Local Auxin Biosynthesis and Its Effects on the Rapid Growth of Moso Bamboo (Phyllostachys edulis)
title_full_unstemmed New Insights Into the Local Auxin Biosynthesis and Its Effects on the Rapid Growth of Moso Bamboo (Phyllostachys edulis)
title_short New Insights Into the Local Auxin Biosynthesis and Its Effects on the Rapid Growth of Moso Bamboo (Phyllostachys edulis)
title_sort new insights into the local auxin biosynthesis and its effects on the rapid growth of moso bamboo (phyllostachys edulis)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9111533/
https://www.ncbi.nlm.nih.gov/pubmed/35592571
http://dx.doi.org/10.3389/fpls.2022.858686
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