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SARS‐CoV‐2 detection in bioaerosols using a liquid impinger collector and ddPCR
The airborne route is the dominant form of COVID‐19 transmission, and therefore, the development of methodologies to quantify SARS‐CoV‐2 in bioaerosols is needed. We aimed to identify SARS‐CoV‐2 in bioaerosols by using a highly efficient sampler for the collection of 1–3 µm particles, followed by a...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9111801/ https://www.ncbi.nlm.nih.gov/pubmed/35225399 http://dx.doi.org/10.1111/ina.13002 |
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author | Truyols Vives, Joan Muncunill, Josep Toledo Pons, Núria Baldoví, Herme G. Sala Llinàs, Ernest Mercader Barceló, Josep |
author_facet | Truyols Vives, Joan Muncunill, Josep Toledo Pons, Núria Baldoví, Herme G. Sala Llinàs, Ernest Mercader Barceló, Josep |
author_sort | Truyols Vives, Joan |
collection | PubMed |
description | The airborne route is the dominant form of COVID‐19 transmission, and therefore, the development of methodologies to quantify SARS‐CoV‐2 in bioaerosols is needed. We aimed to identify SARS‐CoV‐2 in bioaerosols by using a highly efficient sampler for the collection of 1–3 µm particles, followed by a highly sensitive detection method. 65 bioaerosol samples were collected in hospital rooms in the presence of a COVID‐19 patient using a liquid impinger sampler. The SARS‐CoV‐2 genome was detected by ddPCR using different primer/probe sets. 44.6% of the samples resulted positive for SARS‐CoV‐2 following this protocol. By increasing the sampled air volume from 339 to 650 L, the percentage of positive samples went from 41% to 50%. We detected five times less positives with a commercial one‐step RT‐PCR assay. However, the selection of primer/probe sets might be one of the most determining factor for bioaerosol SARS‐CoV‐2 detection since with the ORF1ab set more than 40% of the samples were positive, compared to <10% with other sets. In conclusion, the use of a liquid impinger collector and ddPCR is an adequate strategy to detect SARS‐CoV‐2 in bioaerosols. However, there are still some methodological aspects that must be adjusted to optimize and standardize a definitive protocol. |
format | Online Article Text |
id | pubmed-9111801 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91118012022-05-17 SARS‐CoV‐2 detection in bioaerosols using a liquid impinger collector and ddPCR Truyols Vives, Joan Muncunill, Josep Toledo Pons, Núria Baldoví, Herme G. Sala Llinàs, Ernest Mercader Barceló, Josep Indoor Air Original Articles The airborne route is the dominant form of COVID‐19 transmission, and therefore, the development of methodologies to quantify SARS‐CoV‐2 in bioaerosols is needed. We aimed to identify SARS‐CoV‐2 in bioaerosols by using a highly efficient sampler for the collection of 1–3 µm particles, followed by a highly sensitive detection method. 65 bioaerosol samples were collected in hospital rooms in the presence of a COVID‐19 patient using a liquid impinger sampler. The SARS‐CoV‐2 genome was detected by ddPCR using different primer/probe sets. 44.6% of the samples resulted positive for SARS‐CoV‐2 following this protocol. By increasing the sampled air volume from 339 to 650 L, the percentage of positive samples went from 41% to 50%. We detected five times less positives with a commercial one‐step RT‐PCR assay. However, the selection of primer/probe sets might be one of the most determining factor for bioaerosol SARS‐CoV‐2 detection since with the ORF1ab set more than 40% of the samples were positive, compared to <10% with other sets. In conclusion, the use of a liquid impinger collector and ddPCR is an adequate strategy to detect SARS‐CoV‐2 in bioaerosols. However, there are still some methodological aspects that must be adjusted to optimize and standardize a definitive protocol. John Wiley and Sons Inc. 2022-02-21 2022-02 /pmc/articles/PMC9111801/ /pubmed/35225399 http://dx.doi.org/10.1111/ina.13002 Text en © 2022 The Authors. Indoor Air published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Truyols Vives, Joan Muncunill, Josep Toledo Pons, Núria Baldoví, Herme G. Sala Llinàs, Ernest Mercader Barceló, Josep SARS‐CoV‐2 detection in bioaerosols using a liquid impinger collector and ddPCR |
title | SARS‐CoV‐2 detection in bioaerosols using a liquid impinger collector and ddPCR |
title_full | SARS‐CoV‐2 detection in bioaerosols using a liquid impinger collector and ddPCR |
title_fullStr | SARS‐CoV‐2 detection in bioaerosols using a liquid impinger collector and ddPCR |
title_full_unstemmed | SARS‐CoV‐2 detection in bioaerosols using a liquid impinger collector and ddPCR |
title_short | SARS‐CoV‐2 detection in bioaerosols using a liquid impinger collector and ddPCR |
title_sort | sars‐cov‐2 detection in bioaerosols using a liquid impinger collector and ddpcr |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9111801/ https://www.ncbi.nlm.nih.gov/pubmed/35225399 http://dx.doi.org/10.1111/ina.13002 |
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