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A report on digitised neuronal tracing method to study neurons in their entirety
Conventional camera lucida (CL) aided neuronal tracing technique for studying neural plasticity is a demanding procedure. Stereo Investigator-Neurolucida enabled neuronal tracing system is not accessible to all researchers. This necessitates alternate simple and less challenging digitised neuronal t...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9111970/ https://www.ncbi.nlm.nih.gov/pubmed/35592463 http://dx.doi.org/10.1016/j.mex.2022.101715 |
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author | Ali, Zeeshan Sivakumar, G. Prabhu, Krishnamoorthi Suryavanshi, Chinmay Ajit Narayanan, Sareesh Naduvil |
author_facet | Ali, Zeeshan Sivakumar, G. Prabhu, Krishnamoorthi Suryavanshi, Chinmay Ajit Narayanan, Sareesh Naduvil |
author_sort | Ali, Zeeshan |
collection | PubMed |
description | Conventional camera lucida (CL) aided neuronal tracing technique for studying neural plasticity is a demanding procedure. Stereo Investigator-Neurolucida enabled neuronal tracing system is not accessible to all researchers. This necessitates alternate simple and less challenging digitised neuronal tracing methods. This report describes a novel digitised neuronal tracing method using widefield microscopy, and its effectiveness is compared with the traditional camera lucida aided neuronal tracing method. Golgi-Cox stained hippocampal cornu ammonis area-3 (CA3) pyramidal neuron photomicrographs were serially captured at a depth of every 2µm in the z-axis by a wide field microscope from the point of appearance to the disappearance. These images were stacked along the axis perpendicular to the image plane to reconstruct the neuron in its entirety, digitally traced and dendritic quantification was performed using open source software. The same neurons were manually traced using camera lucida, and Sholl analysis was done manually to quantify the dendritic arborisation pattern. The dendritic quantification data were not significantly different in both methods. Hence, the technology-enabled, less demanding, and equally accurate neuronal tracing can be adopted instead of manual tracing and analysis of neurons. • A simple digitised neuronal tracing method is described. • It is fast, rigorous, and comparable to traditional tracing techniques. • Helps the researcher to repeatedly probe data to reduce errors. |
format | Online Article Text |
id | pubmed-9111970 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-91119702022-05-18 A report on digitised neuronal tracing method to study neurons in their entirety Ali, Zeeshan Sivakumar, G. Prabhu, Krishnamoorthi Suryavanshi, Chinmay Ajit Narayanan, Sareesh Naduvil MethodsX Method Article Conventional camera lucida (CL) aided neuronal tracing technique for studying neural plasticity is a demanding procedure. Stereo Investigator-Neurolucida enabled neuronal tracing system is not accessible to all researchers. This necessitates alternate simple and less challenging digitised neuronal tracing methods. This report describes a novel digitised neuronal tracing method using widefield microscopy, and its effectiveness is compared with the traditional camera lucida aided neuronal tracing method. Golgi-Cox stained hippocampal cornu ammonis area-3 (CA3) pyramidal neuron photomicrographs were serially captured at a depth of every 2µm in the z-axis by a wide field microscope from the point of appearance to the disappearance. These images were stacked along the axis perpendicular to the image plane to reconstruct the neuron in its entirety, digitally traced and dendritic quantification was performed using open source software. The same neurons were manually traced using camera lucida, and Sholl analysis was done manually to quantify the dendritic arborisation pattern. The dendritic quantification data were not significantly different in both methods. Hence, the technology-enabled, less demanding, and equally accurate neuronal tracing can be adopted instead of manual tracing and analysis of neurons. • A simple digitised neuronal tracing method is described. • It is fast, rigorous, and comparable to traditional tracing techniques. • Helps the researcher to repeatedly probe data to reduce errors. Elsevier 2022-04-29 /pmc/articles/PMC9111970/ /pubmed/35592463 http://dx.doi.org/10.1016/j.mex.2022.101715 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Method Article Ali, Zeeshan Sivakumar, G. Prabhu, Krishnamoorthi Suryavanshi, Chinmay Ajit Narayanan, Sareesh Naduvil A report on digitised neuronal tracing method to study neurons in their entirety |
title | A report on digitised neuronal tracing method to study neurons in their entirety |
title_full | A report on digitised neuronal tracing method to study neurons in their entirety |
title_fullStr | A report on digitised neuronal tracing method to study neurons in their entirety |
title_full_unstemmed | A report on digitised neuronal tracing method to study neurons in their entirety |
title_short | A report on digitised neuronal tracing method to study neurons in their entirety |
title_sort | report on digitised neuronal tracing method to study neurons in their entirety |
topic | Method Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9111970/ https://www.ncbi.nlm.nih.gov/pubmed/35592463 http://dx.doi.org/10.1016/j.mex.2022.101715 |
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