Degron tagging of BleoR and other antibiotic-resistance genes selects for higher expression of linked transgenes and improved exosome engineering

Five antibiotic resistance (AR) genes have been used to select for transgenic eukaryotic cell lines, with the BleoR, PuroR, HygR, NeoR, and BsdR cassettes conferring resistance to zeocin, puromycin, hygromycin, geneticin/G418, and blasticidin, respectively. We recently demonstrated that each AR gene...

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Detalles Bibliográficos
Autores principales: Tsai, Shang Jui, Ai, Yiwei, Guo, Chenxu, Gould, Stephen J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9111990/
https://www.ncbi.nlm.nih.gov/pubmed/35314197
http://dx.doi.org/10.1016/j.jbc.2022.101846

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9111990/
https://www.ncbi.nlm.nih.gov/pubmed/35314197
http://dx.doi.org/10.1016/j.jbc.2022.101846

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