A dual conditional CRISPR-Cas9 system to activate gene editing and reduce off-target effects in human stem cells

The CRISPR-Cas9 system has emerged as a powerful and efficient tool for genome editing. An important drawback of the CRISPR-Cas9 system is the constitutive endonuclease activity when Cas9 endonuclease and its sgRNA are co-expressed. This constitutive activity results in undesirable off-target effect...

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Detalles Bibliográficos
Autores principales: Park, Seung Bum, Uchida, Takuro, Tilson, Samantha, Hu, Zongyi, Ma, Christopher D., Leek, Madeleine, Eichner, Michael, Hong, So Gun, Liang, T. Jake
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2022
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9112054/
https://www.ncbi.nlm.nih.gov/pubmed/35615005
http://dx.doi.org/10.1016/j.omtn.2022.04.013

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