Protocol to generate xenogeneic-free/serum-free human dental pulp stem cells

Human dental pulp stem cell (hDPSCs)-based therapy is a feasible option for regenerative medicine, such as dental pulp regeneration. Here, we show the steps needed to colony-forming unit-fibroblasts (CFU-F)-based isolation, expansion, and cryopreservation of hDPSCs for manufacturing clinical-grade p...

Descripción completa

Detalles Bibliográficos
Autores principales: Sonoda, Soichiro, Yamaza, Haruyoshi, Yoshimaru, Koichiro, Taguchi, Tomoaki, Yamaza, Takayoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9112100/
https://www.ncbi.nlm.nih.gov/pubmed/35592060
http://dx.doi.org/10.1016/j.xpro.2022.101386
Descripción
Sumario:Human dental pulp stem cell (hDPSCs)-based therapy is a feasible option for regenerative medicine, such as dental pulp regeneration. Here, we show the steps needed to colony-forming unit-fibroblasts (CFU-F)-based isolation, expansion, and cryopreservation of hDPSCs for manufacturing clinical-grade products under a xenogeneic-free/serum-free condition. We also demonstrate the characterization of hDPSCs by CFU-F, flow cytometric, and in vitro multipotent assays. For complete details on the use and execution of this protocol, please refer to Iwanaka et al. (2020).

Ejemplares similares