Protocol to generate xenogeneic-free/serum-free human dental pulp stem cells

Human dental pulp stem cell (hDPSCs)-based therapy is a feasible option for regenerative medicine, such as dental pulp regeneration. Here, we show the steps needed to colony-forming unit-fibroblasts (CFU-F)-based isolation, expansion, and cryopreservation of hDPSCs for manufacturing clinical-grade p...

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Autores principales: Sonoda, Soichiro, Yamaza, Haruyoshi, Yoshimaru, Koichiro, Taguchi, Tomoaki, Yamaza, Takayoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9112100/
https://www.ncbi.nlm.nih.gov/pubmed/35592060
http://dx.doi.org/10.1016/j.xpro.2022.101386
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author Sonoda, Soichiro
Yamaza, Haruyoshi
Yoshimaru, Koichiro
Taguchi, Tomoaki
Yamaza, Takayoshi
author_facet Sonoda, Soichiro
Yamaza, Haruyoshi
Yoshimaru, Koichiro
Taguchi, Tomoaki
Yamaza, Takayoshi
author_sort Sonoda, Soichiro
collection PubMed
description Human dental pulp stem cell (hDPSCs)-based therapy is a feasible option for regenerative medicine, such as dental pulp regeneration. Here, we show the steps needed to colony-forming unit-fibroblasts (CFU-F)-based isolation, expansion, and cryopreservation of hDPSCs for manufacturing clinical-grade products under a xenogeneic-free/serum-free condition. We also demonstrate the characterization of hDPSCs by CFU-F, flow cytometric, and in vitro multipotent assays. For complete details on the use and execution of this protocol, please refer to Iwanaka et al. (2020).
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spelling pubmed-91121002022-05-18 Protocol to generate xenogeneic-free/serum-free human dental pulp stem cells Sonoda, Soichiro Yamaza, Haruyoshi Yoshimaru, Koichiro Taguchi, Tomoaki Yamaza, Takayoshi STAR Protoc Protocol Human dental pulp stem cell (hDPSCs)-based therapy is a feasible option for regenerative medicine, such as dental pulp regeneration. Here, we show the steps needed to colony-forming unit-fibroblasts (CFU-F)-based isolation, expansion, and cryopreservation of hDPSCs for manufacturing clinical-grade products under a xenogeneic-free/serum-free condition. We also demonstrate the characterization of hDPSCs by CFU-F, flow cytometric, and in vitro multipotent assays. For complete details on the use and execution of this protocol, please refer to Iwanaka et al. (2020). Elsevier 2022-05-13 /pmc/articles/PMC9112100/ /pubmed/35592060 http://dx.doi.org/10.1016/j.xpro.2022.101386 Text en © 2022. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Sonoda, Soichiro
Yamaza, Haruyoshi
Yoshimaru, Koichiro
Taguchi, Tomoaki
Yamaza, Takayoshi
Protocol to generate xenogeneic-free/serum-free human dental pulp stem cells
title Protocol to generate xenogeneic-free/serum-free human dental pulp stem cells
title_full Protocol to generate xenogeneic-free/serum-free human dental pulp stem cells
title_fullStr Protocol to generate xenogeneic-free/serum-free human dental pulp stem cells
title_full_unstemmed Protocol to generate xenogeneic-free/serum-free human dental pulp stem cells
title_short Protocol to generate xenogeneic-free/serum-free human dental pulp stem cells
title_sort protocol to generate xenogeneic-free/serum-free human dental pulp stem cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9112100/
https://www.ncbi.nlm.nih.gov/pubmed/35592060
http://dx.doi.org/10.1016/j.xpro.2022.101386
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