Making Antimicrobial Susceptibility Testing More Physiologically Relevant with Bicarbonate?

Azithromycin is a clinically important drug for treating invasive salmonellosis despite poor activity in laboratory assays for MIC. Addition of the main buffer in blood, bicarbonate, has been proposed for more physiologically relevant and more predictive testing conditions. However, we show here tha...

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Autores principales: Hinnu, Mariliis, Putrinš, Marta, Kogermann, Karin, Bumann, Dirk, Tenson, Tanel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Susceptibility
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9112938/
https://www.ncbi.nlm.nih.gov/pubmed/35435706
http://dx.doi.org/10.1128/aac.02412-21
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author Hinnu, Mariliis
Putrinš, Marta
Kogermann, Karin
Bumann, Dirk
Tenson, Tanel
author_facet Hinnu, Mariliis
Putrinš, Marta
Kogermann, Karin
Bumann, Dirk
Tenson, Tanel
author_sort Hinnu, Mariliis
collection PubMed
description Azithromycin is a clinically important drug for treating invasive salmonellosis despite poor activity in laboratory assays for MIC. Addition of the main buffer in blood, bicarbonate, has been proposed for more physiologically relevant and more predictive testing conditions. However, we show here that bicarbonate-triggered lowering of azithromycin MIC is entirely due to alkalization of insufficiently buffered media. In addition, bicarbonate is unlikely to be altering efflux pump activity.
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spelling pubmed-91129382022-05-18 Making Antimicrobial Susceptibility Testing More Physiologically Relevant with Bicarbonate? Hinnu, Mariliis Putrinš, Marta Kogermann, Karin Bumann, Dirk Tenson, Tanel Antimicrob Agents Chemother Susceptibility Azithromycin is a clinically important drug for treating invasive salmonellosis despite poor activity in laboratory assays for MIC. Addition of the main buffer in blood, bicarbonate, has been proposed for more physiologically relevant and more predictive testing conditions. However, we show here that bicarbonate-triggered lowering of azithromycin MIC is entirely due to alkalization of insufficiently buffered media. In addition, bicarbonate is unlikely to be altering efflux pump activity. American Society for Microbiology 2022-04-18 /pmc/articles/PMC9112938/ /pubmed/35435706 http://dx.doi.org/10.1128/aac.02412-21 Text en Copyright © 2022 Hinnu et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Susceptibility
Hinnu, Mariliis
Putrinš, Marta
Kogermann, Karin
Bumann, Dirk
Tenson, Tanel
Making Antimicrobial Susceptibility Testing More Physiologically Relevant with Bicarbonate?
title Making Antimicrobial Susceptibility Testing More Physiologically Relevant with Bicarbonate?
title_full Making Antimicrobial Susceptibility Testing More Physiologically Relevant with Bicarbonate?
title_fullStr Making Antimicrobial Susceptibility Testing More Physiologically Relevant with Bicarbonate?
title_full_unstemmed Making Antimicrobial Susceptibility Testing More Physiologically Relevant with Bicarbonate?
title_short Making Antimicrobial Susceptibility Testing More Physiologically Relevant with Bicarbonate?
title_sort making antimicrobial susceptibility testing more physiologically relevant with bicarbonate?
topic Susceptibility
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9112938/
https://www.ncbi.nlm.nih.gov/pubmed/35435706
http://dx.doi.org/10.1128/aac.02412-21
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