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Cc2d1b Contributes to the Regulation of Developmental Myelination in the Central Nervous System

BACKGROUND: Numerous studies have indicated that myelination is the result of the interplay between extracellular signals and an intricate network of transcription factors. Yet, the identification and characterization of the full repertoire of transcription factors that modulate myelination are stil...

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Autores principales: Acheta, Jenica, Hong, Jiayue, Jeanette, Haley, Brar, Simrandeep, Yalamanchili, Anish, Feltri, M. Laura, Manzini, M. Chiara, Belin, Sophie, Poitelon, Yannick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113218/
https://www.ncbi.nlm.nih.gov/pubmed/35592111
http://dx.doi.org/10.3389/fnmol.2022.881571
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author Acheta, Jenica
Hong, Jiayue
Jeanette, Haley
Brar, Simrandeep
Yalamanchili, Anish
Feltri, M. Laura
Manzini, M. Chiara
Belin, Sophie
Poitelon, Yannick
author_facet Acheta, Jenica
Hong, Jiayue
Jeanette, Haley
Brar, Simrandeep
Yalamanchili, Anish
Feltri, M. Laura
Manzini, M. Chiara
Belin, Sophie
Poitelon, Yannick
author_sort Acheta, Jenica
collection PubMed
description BACKGROUND: Numerous studies have indicated that myelination is the result of the interplay between extracellular signals and an intricate network of transcription factors. Yet, the identification and characterization of the full repertoire of transcription factors that modulate myelination are still incomplete. CC2D1B is a member of the Lgd/CC2D1 family of proteins highly expressed in myelinating cells in the central and peripheral nervous systems. In addition, the absence of CC2D1B limits myelin formation in vitro. Here we propose to delineate the function of CC2D1B in myelinating cells during developmental myelination in vivo in the central and peripheral nervous systems. METHODS: We used a Cc2d1b constitutive knockout mouse model and then performed morphological analyses on semithin sections of sciatic nerves and electron micrographs of optic nerves. We also performed immunohistological studies on coronal brain sections. All analyses were performed at 30 days of age. RESULTS: In the peripheral nervous system, animals ablated for Cc2d1b did not show any myelin thickness difference compared to control animals. In the central nervous system, immunohistological studies did not show any difference in the number of oligodendrocytes or the level of myelin proteins in the cortex, corpus callosum, and striatum. However, optic nerves showed a hypomyelination (0.844 ± 0.022) compared to control animals (0.832 ± 0.016) of large diameter myelinated fibers. CONCLUSIONS: We found that CC2D1B plays a role in developmental myelination in the central nervous system. These results suggest that CC2D1B could contribute to gene regulation during oligodendrocytes myelination in optic nerves.
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spelling pubmed-91132182022-05-18 Cc2d1b Contributes to the Regulation of Developmental Myelination in the Central Nervous System Acheta, Jenica Hong, Jiayue Jeanette, Haley Brar, Simrandeep Yalamanchili, Anish Feltri, M. Laura Manzini, M. Chiara Belin, Sophie Poitelon, Yannick Front Mol Neurosci Molecular Neuroscience BACKGROUND: Numerous studies have indicated that myelination is the result of the interplay between extracellular signals and an intricate network of transcription factors. Yet, the identification and characterization of the full repertoire of transcription factors that modulate myelination are still incomplete. CC2D1B is a member of the Lgd/CC2D1 family of proteins highly expressed in myelinating cells in the central and peripheral nervous systems. In addition, the absence of CC2D1B limits myelin formation in vitro. Here we propose to delineate the function of CC2D1B in myelinating cells during developmental myelination in vivo in the central and peripheral nervous systems. METHODS: We used a Cc2d1b constitutive knockout mouse model and then performed morphological analyses on semithin sections of sciatic nerves and electron micrographs of optic nerves. We also performed immunohistological studies on coronal brain sections. All analyses were performed at 30 days of age. RESULTS: In the peripheral nervous system, animals ablated for Cc2d1b did not show any myelin thickness difference compared to control animals. In the central nervous system, immunohistological studies did not show any difference in the number of oligodendrocytes or the level of myelin proteins in the cortex, corpus callosum, and striatum. However, optic nerves showed a hypomyelination (0.844 ± 0.022) compared to control animals (0.832 ± 0.016) of large diameter myelinated fibers. CONCLUSIONS: We found that CC2D1B plays a role in developmental myelination in the central nervous system. These results suggest that CC2D1B could contribute to gene regulation during oligodendrocytes myelination in optic nerves. Frontiers Media S.A. 2022-05-03 /pmc/articles/PMC9113218/ /pubmed/35592111 http://dx.doi.org/10.3389/fnmol.2022.881571 Text en Copyright © 2022 Acheta, Hong, Jeanette, Brar, Yalamanchili, Feltri, Manzini, Belin and Poitelon. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Neuroscience
Acheta, Jenica
Hong, Jiayue
Jeanette, Haley
Brar, Simrandeep
Yalamanchili, Anish
Feltri, M. Laura
Manzini, M. Chiara
Belin, Sophie
Poitelon, Yannick
Cc2d1b Contributes to the Regulation of Developmental Myelination in the Central Nervous System
title Cc2d1b Contributes to the Regulation of Developmental Myelination in the Central Nervous System
title_full Cc2d1b Contributes to the Regulation of Developmental Myelination in the Central Nervous System
title_fullStr Cc2d1b Contributes to the Regulation of Developmental Myelination in the Central Nervous System
title_full_unstemmed Cc2d1b Contributes to the Regulation of Developmental Myelination in the Central Nervous System
title_short Cc2d1b Contributes to the Regulation of Developmental Myelination in the Central Nervous System
title_sort cc2d1b contributes to the regulation of developmental myelination in the central nervous system
topic Molecular Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113218/
https://www.ncbi.nlm.nih.gov/pubmed/35592111
http://dx.doi.org/10.3389/fnmol.2022.881571
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