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Sugar signal mediates flavonoid biosynthesis in tea leaves
Sugar metabolism and flavonoid biosynthesis vary with the development of tea leaves. In order to understand the regulatory mechanisms underlying the associations between them, a comprehensive transcriptomic analysis of naturally growing tea leaves at different stages of maturity was carried out. Bas...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113228/ https://www.ncbi.nlm.nih.gov/pubmed/35591928 http://dx.doi.org/10.1093/hr/uhac049 |
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author | Lv, Yi-Qing Li, Da Wu, Liang-Yu Zhu, Yu-Meng Ye, Ying Zheng, Xin-Qiang Lu, Jian-Liang Liang, Yue-Rong Li, Qing-Sheng Ye, Jian-Hui |
author_facet | Lv, Yi-Qing Li, Da Wu, Liang-Yu Zhu, Yu-Meng Ye, Ying Zheng, Xin-Qiang Lu, Jian-Liang Liang, Yue-Rong Li, Qing-Sheng Ye, Jian-Hui |
author_sort | Lv, Yi-Qing |
collection | PubMed |
description | Sugar metabolism and flavonoid biosynthesis vary with the development of tea leaves. In order to understand the regulatory mechanisms underlying the associations between them, a comprehensive transcriptomic analysis of naturally growing tea leaves at different stages of maturity was carried out. Based on weighted gene coexpression network analysis, the key gene modules (Modules 2 and 3) related to the varying relationship between sugar metabolism and flavonoid biosynthesis as well as the corresponding hub genes were obtained. KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis showed that the transcription factors (TFs) in Modules 2 and 3 were mainly enriched in the pathway of plant hormone signal transduction. An in vitro study showed that the transcriptional levels of ERF1B-like TF for hexokinase inhibitor and sucrose treatments were upregulated, being respectively 28.1- and 30.2-fold higher than in the control, suggesting that ERF1B-like TFs participate in the sugar-induced regulation of flavonoid biosynthesis. The results of yeast one-hybrid and dual-luciferase assays demonstrated that CsF3′H, encoding flavonoid 3′-hydroxylase, was the target flavonoid biosynthetic gene for CsERF1B-like TF. Our study identified the potential key regulators participating in the metabolism of sugars and flavonoids, providing new insights into the crosstalk between sugar metabolism and flavonoid biosynthesis in tea plants. |
format | Online Article Text |
id | pubmed-9113228 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-91132282022-05-18 Sugar signal mediates flavonoid biosynthesis in tea leaves Lv, Yi-Qing Li, Da Wu, Liang-Yu Zhu, Yu-Meng Ye, Ying Zheng, Xin-Qiang Lu, Jian-Liang Liang, Yue-Rong Li, Qing-Sheng Ye, Jian-Hui Hortic Res Article Sugar metabolism and flavonoid biosynthesis vary with the development of tea leaves. In order to understand the regulatory mechanisms underlying the associations between them, a comprehensive transcriptomic analysis of naturally growing tea leaves at different stages of maturity was carried out. Based on weighted gene coexpression network analysis, the key gene modules (Modules 2 and 3) related to the varying relationship between sugar metabolism and flavonoid biosynthesis as well as the corresponding hub genes were obtained. KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis showed that the transcription factors (TFs) in Modules 2 and 3 were mainly enriched in the pathway of plant hormone signal transduction. An in vitro study showed that the transcriptional levels of ERF1B-like TF for hexokinase inhibitor and sucrose treatments were upregulated, being respectively 28.1- and 30.2-fold higher than in the control, suggesting that ERF1B-like TFs participate in the sugar-induced regulation of flavonoid biosynthesis. The results of yeast one-hybrid and dual-luciferase assays demonstrated that CsF3′H, encoding flavonoid 3′-hydroxylase, was the target flavonoid biosynthetic gene for CsERF1B-like TF. Our study identified the potential key regulators participating in the metabolism of sugars and flavonoids, providing new insights into the crosstalk between sugar metabolism and flavonoid biosynthesis in tea plants. Oxford University Press 2022-03-14 /pmc/articles/PMC9113228/ /pubmed/35591928 http://dx.doi.org/10.1093/hr/uhac049 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Nanjing Agricultural University https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Lv, Yi-Qing Li, Da Wu, Liang-Yu Zhu, Yu-Meng Ye, Ying Zheng, Xin-Qiang Lu, Jian-Liang Liang, Yue-Rong Li, Qing-Sheng Ye, Jian-Hui Sugar signal mediates flavonoid biosynthesis in tea leaves |
title | Sugar signal mediates flavonoid biosynthesis in tea leaves |
title_full | Sugar signal mediates flavonoid biosynthesis in tea leaves |
title_fullStr | Sugar signal mediates flavonoid biosynthesis in tea leaves |
title_full_unstemmed | Sugar signal mediates flavonoid biosynthesis in tea leaves |
title_short | Sugar signal mediates flavonoid biosynthesis in tea leaves |
title_sort | sugar signal mediates flavonoid biosynthesis in tea leaves |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113228/ https://www.ncbi.nlm.nih.gov/pubmed/35591928 http://dx.doi.org/10.1093/hr/uhac049 |
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