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Profile of circulating extracellular vesicles microRNA correlates with the disease activity in granulomatosis with polyangiitis

Granulomatosis with polyangiitis is a chronic systemic inflammation of small vessels characterized by circulating anti-proteinase 3 antibodies. MicroRNAs are short transcripts specifically inhibiting protein translation. Neutrophils can release extracellular vesicles (EVs). In this study, we charact...

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Autores principales: Surmiak, Marcin, Wawrzycka-Adamczyk, Katarzyna, Kosałka-Węgiel, Joanna, Polański, Stanisław, Sanak, Marek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113355/
https://www.ncbi.nlm.nih.gov/pubmed/35380163
http://dx.doi.org/10.1093/cei/uxac022
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author Surmiak, Marcin
Wawrzycka-Adamczyk, Katarzyna
Kosałka-Węgiel, Joanna
Polański, Stanisław
Sanak, Marek
author_facet Surmiak, Marcin
Wawrzycka-Adamczyk, Katarzyna
Kosałka-Węgiel, Joanna
Polański, Stanisław
Sanak, Marek
author_sort Surmiak, Marcin
collection PubMed
description Granulomatosis with polyangiitis is a chronic systemic inflammation of small vessels characterized by circulating anti-proteinase 3 antibodies. MicroRNAs are short transcripts specifically inhibiting protein translation. Neutrophils can release extracellular vesicles (EVs). In this study, we characterized profile of microRNA trafficked by EVs in GPA. Fifty patients with GPA were enrolled in the study, 25 at acute phase and 25 in remission. EVs were isolated from the blood serum, characterized by their number, size distribution. Following unbiased screening for microRNA expression, differentially expressed candidates were measured by quantitative real-time PCR. Circulating DNA-myeloperoxidase complexes and apoptosis-related transcripts in peripheral blood neutrophils were quantified. We identified four differentially expressed microRNAs from EVs in granulomatosis with polyangiitis (GPA). MirRs-223-3p, 664a-3p, and 200b-3p were overexpressed and miR-769-5p suppressed in the disease. A distinction between GPA and healthy controls was the best for miR-223-3p, whereas miR-664a-3p discriminated between active vs. remission of GPA. Correct classification of the disease based on multivariate discriminant analysis was between 92% for acute phase and 85% for all study participants. Bioinformatics tools identified genes transcripts potentially targeted by the microRNAs belonging to pathways of focal adhesion, mTOR signaling and neutrophil extracellular traps formation. Two microRNAs positively correlating with the disease activity were involved in neutrophil extracellular traps formation and apoptosis inhibition. A comprehensive characteristics of microRNAs trafficked in bloodstream inside EVs correlates well with our understanding of the mechanisms of GPA and suggests the importance of EVs in progression of the disease.
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spelling pubmed-91133552022-12-12 Profile of circulating extracellular vesicles microRNA correlates with the disease activity in granulomatosis with polyangiitis Surmiak, Marcin Wawrzycka-Adamczyk, Katarzyna Kosałka-Węgiel, Joanna Polański, Stanisław Sanak, Marek Clin Exp Immunol Research Articles Granulomatosis with polyangiitis is a chronic systemic inflammation of small vessels characterized by circulating anti-proteinase 3 antibodies. MicroRNAs are short transcripts specifically inhibiting protein translation. Neutrophils can release extracellular vesicles (EVs). In this study, we characterized profile of microRNA trafficked by EVs in GPA. Fifty patients with GPA were enrolled in the study, 25 at acute phase and 25 in remission. EVs were isolated from the blood serum, characterized by their number, size distribution. Following unbiased screening for microRNA expression, differentially expressed candidates were measured by quantitative real-time PCR. Circulating DNA-myeloperoxidase complexes and apoptosis-related transcripts in peripheral blood neutrophils were quantified. We identified four differentially expressed microRNAs from EVs in granulomatosis with polyangiitis (GPA). MirRs-223-3p, 664a-3p, and 200b-3p were overexpressed and miR-769-5p suppressed in the disease. A distinction between GPA and healthy controls was the best for miR-223-3p, whereas miR-664a-3p discriminated between active vs. remission of GPA. Correct classification of the disease based on multivariate discriminant analysis was between 92% for acute phase and 85% for all study participants. Bioinformatics tools identified genes transcripts potentially targeted by the microRNAs belonging to pathways of focal adhesion, mTOR signaling and neutrophil extracellular traps formation. Two microRNAs positively correlating with the disease activity were involved in neutrophil extracellular traps formation and apoptosis inhibition. A comprehensive characteristics of microRNAs trafficked in bloodstream inside EVs correlates well with our understanding of the mechanisms of GPA and suggests the importance of EVs in progression of the disease. Oxford University Press 2022-03-03 /pmc/articles/PMC9113355/ /pubmed/35380163 http://dx.doi.org/10.1093/cei/uxac022 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of the British Society for Immunology. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Surmiak, Marcin
Wawrzycka-Adamczyk, Katarzyna
Kosałka-Węgiel, Joanna
Polański, Stanisław
Sanak, Marek
Profile of circulating extracellular vesicles microRNA correlates with the disease activity in granulomatosis with polyangiitis
title Profile of circulating extracellular vesicles microRNA correlates with the disease activity in granulomatosis with polyangiitis
title_full Profile of circulating extracellular vesicles microRNA correlates with the disease activity in granulomatosis with polyangiitis
title_fullStr Profile of circulating extracellular vesicles microRNA correlates with the disease activity in granulomatosis with polyangiitis
title_full_unstemmed Profile of circulating extracellular vesicles microRNA correlates with the disease activity in granulomatosis with polyangiitis
title_short Profile of circulating extracellular vesicles microRNA correlates with the disease activity in granulomatosis with polyangiitis
title_sort profile of circulating extracellular vesicles microrna correlates with the disease activity in granulomatosis with polyangiitis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113355/
https://www.ncbi.nlm.nih.gov/pubmed/35380163
http://dx.doi.org/10.1093/cei/uxac022
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