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Disruption of the Trichoderma reesei gul1 gene stimulates hyphal branching and reduces broth viscosity in cellulase production

Hyphal morphology is considered to have a close relationship with the production level of secreted proteins by filamentous fungi. In this study, the gul1 gene, which encodes a putative mRNA-binding protein, was disrupted in cellulase-producing fungus Trichoderma reesei. The hyphae of Δgul1 strain pr...

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Autores principales: Zhao, Qinqin, Liu, Qin, Wang, Qi, Qin, Yuqi, Zhong, Yaohua, Gao, Liwei, Liu, Guodong, Qu, Yinbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113457/
https://www.ncbi.nlm.nih.gov/pubmed/33693788
http://dx.doi.org/10.1093/jimb/kuab012
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author Zhao, Qinqin
Liu, Qin
Wang, Qi
Qin, Yuqi
Zhong, Yaohua
Gao, Liwei
Liu, Guodong
Qu, Yinbo
author_facet Zhao, Qinqin
Liu, Qin
Wang, Qi
Qin, Yuqi
Zhong, Yaohua
Gao, Liwei
Liu, Guodong
Qu, Yinbo
author_sort Zhao, Qinqin
collection PubMed
description Hyphal morphology is considered to have a close relationship with the production level of secreted proteins by filamentous fungi. In this study, the gul1 gene, which encodes a putative mRNA-binding protein, was disrupted in cellulase-producing fungus Trichoderma reesei. The hyphae of Δgul1 strain produced more lateral branches than the parent strain. Under the condition for cellulase production, disruption of gul1 resulted in smaller mycelial clumps and significantly lower viscosity of fermentation broth. In addition, cellulase production was improved by 22% relative to the parent strain. Transcriptome analysis revealed that a set of genes encoding cell wall remodeling enzymes as well as hydrophobins were differentially expressed in the Δgul1 strain. The results suggest that the regulatory role of gul1 in cell morphogenesis is likely conserved in filamentous fungi. To our knowledge, this is the first report on the engineering of gul1 in an industrially important fungus.
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spelling pubmed-91134572022-06-10 Disruption of the Trichoderma reesei gul1 gene stimulates hyphal branching and reduces broth viscosity in cellulase production Zhao, Qinqin Liu, Qin Wang, Qi Qin, Yuqi Zhong, Yaohua Gao, Liwei Liu, Guodong Qu, Yinbo J Ind Microbiol Biotechnol Genetics and Molecular Biology of Industrial Organisms Hyphal morphology is considered to have a close relationship with the production level of secreted proteins by filamentous fungi. In this study, the gul1 gene, which encodes a putative mRNA-binding protein, was disrupted in cellulase-producing fungus Trichoderma reesei. The hyphae of Δgul1 strain produced more lateral branches than the parent strain. Under the condition for cellulase production, disruption of gul1 resulted in smaller mycelial clumps and significantly lower viscosity of fermentation broth. In addition, cellulase production was improved by 22% relative to the parent strain. Transcriptome analysis revealed that a set of genes encoding cell wall remodeling enzymes as well as hydrophobins were differentially expressed in the Δgul1 strain. The results suggest that the regulatory role of gul1 in cell morphogenesis is likely conserved in filamentous fungi. To our knowledge, this is the first report on the engineering of gul1 in an industrially important fungus. Oxford University Press 2021-02-10 /pmc/articles/PMC9113457/ /pubmed/33693788 http://dx.doi.org/10.1093/jimb/kuab012 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Society of Industrial Microbiology and Biotechnology. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Genetics and Molecular Biology of Industrial Organisms
Zhao, Qinqin
Liu, Qin
Wang, Qi
Qin, Yuqi
Zhong, Yaohua
Gao, Liwei
Liu, Guodong
Qu, Yinbo
Disruption of the Trichoderma reesei gul1 gene stimulates hyphal branching and reduces broth viscosity in cellulase production
title Disruption of the Trichoderma reesei gul1 gene stimulates hyphal branching and reduces broth viscosity in cellulase production
title_full Disruption of the Trichoderma reesei gul1 gene stimulates hyphal branching and reduces broth viscosity in cellulase production
title_fullStr Disruption of the Trichoderma reesei gul1 gene stimulates hyphal branching and reduces broth viscosity in cellulase production
title_full_unstemmed Disruption of the Trichoderma reesei gul1 gene stimulates hyphal branching and reduces broth viscosity in cellulase production
title_short Disruption of the Trichoderma reesei gul1 gene stimulates hyphal branching and reduces broth viscosity in cellulase production
title_sort disruption of the trichoderma reesei gul1 gene stimulates hyphal branching and reduces broth viscosity in cellulase production
topic Genetics and Molecular Biology of Industrial Organisms
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113457/
https://www.ncbi.nlm.nih.gov/pubmed/33693788
http://dx.doi.org/10.1093/jimb/kuab012
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