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Disruption of the Trichoderma reesei gul1 gene stimulates hyphal branching and reduces broth viscosity in cellulase production
Hyphal morphology is considered to have a close relationship with the production level of secreted proteins by filamentous fungi. In this study, the gul1 gene, which encodes a putative mRNA-binding protein, was disrupted in cellulase-producing fungus Trichoderma reesei. The hyphae of Δgul1 strain pr...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113457/ https://www.ncbi.nlm.nih.gov/pubmed/33693788 http://dx.doi.org/10.1093/jimb/kuab012 |
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author | Zhao, Qinqin Liu, Qin Wang, Qi Qin, Yuqi Zhong, Yaohua Gao, Liwei Liu, Guodong Qu, Yinbo |
author_facet | Zhao, Qinqin Liu, Qin Wang, Qi Qin, Yuqi Zhong, Yaohua Gao, Liwei Liu, Guodong Qu, Yinbo |
author_sort | Zhao, Qinqin |
collection | PubMed |
description | Hyphal morphology is considered to have a close relationship with the production level of secreted proteins by filamentous fungi. In this study, the gul1 gene, which encodes a putative mRNA-binding protein, was disrupted in cellulase-producing fungus Trichoderma reesei. The hyphae of Δgul1 strain produced more lateral branches than the parent strain. Under the condition for cellulase production, disruption of gul1 resulted in smaller mycelial clumps and significantly lower viscosity of fermentation broth. In addition, cellulase production was improved by 22% relative to the parent strain. Transcriptome analysis revealed that a set of genes encoding cell wall remodeling enzymes as well as hydrophobins were differentially expressed in the Δgul1 strain. The results suggest that the regulatory role of gul1 in cell morphogenesis is likely conserved in filamentous fungi. To our knowledge, this is the first report on the engineering of gul1 in an industrially important fungus. |
format | Online Article Text |
id | pubmed-9113457 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-91134572022-06-10 Disruption of the Trichoderma reesei gul1 gene stimulates hyphal branching and reduces broth viscosity in cellulase production Zhao, Qinqin Liu, Qin Wang, Qi Qin, Yuqi Zhong, Yaohua Gao, Liwei Liu, Guodong Qu, Yinbo J Ind Microbiol Biotechnol Genetics and Molecular Biology of Industrial Organisms Hyphal morphology is considered to have a close relationship with the production level of secreted proteins by filamentous fungi. In this study, the gul1 gene, which encodes a putative mRNA-binding protein, was disrupted in cellulase-producing fungus Trichoderma reesei. The hyphae of Δgul1 strain produced more lateral branches than the parent strain. Under the condition for cellulase production, disruption of gul1 resulted in smaller mycelial clumps and significantly lower viscosity of fermentation broth. In addition, cellulase production was improved by 22% relative to the parent strain. Transcriptome analysis revealed that a set of genes encoding cell wall remodeling enzymes as well as hydrophobins were differentially expressed in the Δgul1 strain. The results suggest that the regulatory role of gul1 in cell morphogenesis is likely conserved in filamentous fungi. To our knowledge, this is the first report on the engineering of gul1 in an industrially important fungus. Oxford University Press 2021-02-10 /pmc/articles/PMC9113457/ /pubmed/33693788 http://dx.doi.org/10.1093/jimb/kuab012 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Society of Industrial Microbiology and Biotechnology. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Genetics and Molecular Biology of Industrial Organisms Zhao, Qinqin Liu, Qin Wang, Qi Qin, Yuqi Zhong, Yaohua Gao, Liwei Liu, Guodong Qu, Yinbo Disruption of the Trichoderma reesei gul1 gene stimulates hyphal branching and reduces broth viscosity in cellulase production |
title | Disruption of the Trichoderma reesei gul1 gene
stimulates hyphal branching and reduces broth viscosity in cellulase
production |
title_full | Disruption of the Trichoderma reesei gul1 gene
stimulates hyphal branching and reduces broth viscosity in cellulase
production |
title_fullStr | Disruption of the Trichoderma reesei gul1 gene
stimulates hyphal branching and reduces broth viscosity in cellulase
production |
title_full_unstemmed | Disruption of the Trichoderma reesei gul1 gene
stimulates hyphal branching and reduces broth viscosity in cellulase
production |
title_short | Disruption of the Trichoderma reesei gul1 gene
stimulates hyphal branching and reduces broth viscosity in cellulase
production |
title_sort | disruption of the trichoderma reesei gul1 gene
stimulates hyphal branching and reduces broth viscosity in cellulase
production |
topic | Genetics and Molecular Biology of Industrial Organisms |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113457/ https://www.ncbi.nlm.nih.gov/pubmed/33693788 http://dx.doi.org/10.1093/jimb/kuab012 |
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