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Oral Vaccination of Mice With Trichinella spiralis Putative Serine Protease and Murine Interleukin-4 DNA Delivered by Invasive Lactiplantibacillus plantarum Elicits Protective Immunity

Trichinellosis is a serious zoonotic parasitic disease caused by Trichinella spiralis (T. spiralis) that causes considerable economic losses for the global pig breeding and food industries. As such, there is an urgent need for a vaccine that can prevent T. spiralis infection. Previous studies have r...

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Autores principales: Xue, Ying, Zhang, Bo, Wang, Nan, Huang, Hai-Bin, Quan, Yu, Lu, Hui-Nan, Zhu, Zhi-Yu, Li, Jun-Yi, Pan, Tian-Xu, Tang, Yue, Jiang, Yan-Long, Shi, Chun-Wei, Yang, Gui-Lian, Wang, Chun-Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113538/
https://www.ncbi.nlm.nih.gov/pubmed/35591986
http://dx.doi.org/10.3389/fmicb.2022.859243
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author Xue, Ying
Zhang, Bo
Wang, Nan
Huang, Hai-Bin
Quan, Yu
Lu, Hui-Nan
Zhu, Zhi-Yu
Li, Jun-Yi
Pan, Tian-Xu
Tang, Yue
Jiang, Yan-Long
Shi, Chun-Wei
Yang, Gui-Lian
Wang, Chun-Feng
author_facet Xue, Ying
Zhang, Bo
Wang, Nan
Huang, Hai-Bin
Quan, Yu
Lu, Hui-Nan
Zhu, Zhi-Yu
Li, Jun-Yi
Pan, Tian-Xu
Tang, Yue
Jiang, Yan-Long
Shi, Chun-Wei
Yang, Gui-Lian
Wang, Chun-Feng
author_sort Xue, Ying
collection PubMed
description Trichinellosis is a serious zoonotic parasitic disease caused by Trichinella spiralis (T. spiralis) that causes considerable economic losses for the global pig breeding and food industries. As such, there is an urgent need for a vaccine that can prevent T. spiralis infection. Previous studies have reported that recombinant invasive Lactococcus lactis (LL) expressing Staphylococcus aureus fibronectin binding protein A (LL-FnBPA+) can transfer DNA vaccines directly to dendritic cells (DCs) across an epithelial cell monolayer, leading to significantly higher amounts of heterologous protein expression compared to non-invasive Lactococcus lactis. In this study, the invasive bacterium Lactiplantibacillus plantarum (L. plantarum) expressing FnBPA was used as a carrier to deliver a novel oral DNA vaccine consisting of T. spiralis adult putative serine protease (Ts-ADpsp) and murine interleukin (IL)-4 DNA to mouse intestinal epithelial cells. Experimental mice were orally immunized 3 times at 10-day intervals. At 10 days after the last vaccination, mice were challenged with 350 T. spiralis infective larvae by oral inoculation. Immunization with invasive L. plantarum harboring pValac-Ts-ADpsp/pSIP409-FnBPA induced the production of anti-Ts-ADpsp-specific IgG of serum, type 1 and 2 helper T cell cytokines of mesenteric lymph node (MLN) and spleen, secreted (s) IgA of intestinal lavage, and decreased T. spiralis burden and intestinal damage compared to immunization with non-invasive L. plantarum expressing Ts-ADpsp (pValac-Ts-ADpsp/pSIP409). Thus, invasive L. plantarum expressing FnBPA and IL-4 stimulates both mucosal and cellular immune response to protect against T. spiralis infection, highlighting its therapeutic potential as an effective DNA vaccine for trichinellosis.
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spelling pubmed-91135382022-05-18 Oral Vaccination of Mice With Trichinella spiralis Putative Serine Protease and Murine Interleukin-4 DNA Delivered by Invasive Lactiplantibacillus plantarum Elicits Protective Immunity Xue, Ying Zhang, Bo Wang, Nan Huang, Hai-Bin Quan, Yu Lu, Hui-Nan Zhu, Zhi-Yu Li, Jun-Yi Pan, Tian-Xu Tang, Yue Jiang, Yan-Long Shi, Chun-Wei Yang, Gui-Lian Wang, Chun-Feng Front Microbiol Microbiology Trichinellosis is a serious zoonotic parasitic disease caused by Trichinella spiralis (T. spiralis) that causes considerable economic losses for the global pig breeding and food industries. As such, there is an urgent need for a vaccine that can prevent T. spiralis infection. Previous studies have reported that recombinant invasive Lactococcus lactis (LL) expressing Staphylococcus aureus fibronectin binding protein A (LL-FnBPA+) can transfer DNA vaccines directly to dendritic cells (DCs) across an epithelial cell monolayer, leading to significantly higher amounts of heterologous protein expression compared to non-invasive Lactococcus lactis. In this study, the invasive bacterium Lactiplantibacillus plantarum (L. plantarum) expressing FnBPA was used as a carrier to deliver a novel oral DNA vaccine consisting of T. spiralis adult putative serine protease (Ts-ADpsp) and murine interleukin (IL)-4 DNA to mouse intestinal epithelial cells. Experimental mice were orally immunized 3 times at 10-day intervals. At 10 days after the last vaccination, mice were challenged with 350 T. spiralis infective larvae by oral inoculation. Immunization with invasive L. plantarum harboring pValac-Ts-ADpsp/pSIP409-FnBPA induced the production of anti-Ts-ADpsp-specific IgG of serum, type 1 and 2 helper T cell cytokines of mesenteric lymph node (MLN) and spleen, secreted (s) IgA of intestinal lavage, and decreased T. spiralis burden and intestinal damage compared to immunization with non-invasive L. plantarum expressing Ts-ADpsp (pValac-Ts-ADpsp/pSIP409). Thus, invasive L. plantarum expressing FnBPA and IL-4 stimulates both mucosal and cellular immune response to protect against T. spiralis infection, highlighting its therapeutic potential as an effective DNA vaccine for trichinellosis. Frontiers Media S.A. 2022-05-03 /pmc/articles/PMC9113538/ /pubmed/35591986 http://dx.doi.org/10.3389/fmicb.2022.859243 Text en Copyright © 2022 Xue, Zhang, Wang, Huang, Quan, Lu, Zhu, Li, Pan, Tang, Jiang, Shi, Yang and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Xue, Ying
Zhang, Bo
Wang, Nan
Huang, Hai-Bin
Quan, Yu
Lu, Hui-Nan
Zhu, Zhi-Yu
Li, Jun-Yi
Pan, Tian-Xu
Tang, Yue
Jiang, Yan-Long
Shi, Chun-Wei
Yang, Gui-Lian
Wang, Chun-Feng
Oral Vaccination of Mice With Trichinella spiralis Putative Serine Protease and Murine Interleukin-4 DNA Delivered by Invasive Lactiplantibacillus plantarum Elicits Protective Immunity
title Oral Vaccination of Mice With Trichinella spiralis Putative Serine Protease and Murine Interleukin-4 DNA Delivered by Invasive Lactiplantibacillus plantarum Elicits Protective Immunity
title_full Oral Vaccination of Mice With Trichinella spiralis Putative Serine Protease and Murine Interleukin-4 DNA Delivered by Invasive Lactiplantibacillus plantarum Elicits Protective Immunity
title_fullStr Oral Vaccination of Mice With Trichinella spiralis Putative Serine Protease and Murine Interleukin-4 DNA Delivered by Invasive Lactiplantibacillus plantarum Elicits Protective Immunity
title_full_unstemmed Oral Vaccination of Mice With Trichinella spiralis Putative Serine Protease and Murine Interleukin-4 DNA Delivered by Invasive Lactiplantibacillus plantarum Elicits Protective Immunity
title_short Oral Vaccination of Mice With Trichinella spiralis Putative Serine Protease and Murine Interleukin-4 DNA Delivered by Invasive Lactiplantibacillus plantarum Elicits Protective Immunity
title_sort oral vaccination of mice with trichinella spiralis putative serine protease and murine interleukin-4 dna delivered by invasive lactiplantibacillus plantarum elicits protective immunity
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113538/
https://www.ncbi.nlm.nih.gov/pubmed/35591986
http://dx.doi.org/10.3389/fmicb.2022.859243
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