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Serological and Molecular Characterization of Hepatitis B Virus Infection in Gastric Cancer

Hepatitis B virus (HBV) infection has been reported to be associated with gastric cancer (GC). Nonetheless, no study has revealed the role of HBV infection in the survival of patients with GC, and the mutation profiles of HBV-infected patients with GC have never been documented. Here, we performed a...

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Autores principales: Li, Mengge, Wu, Shusheng, Luo, Huiqin, Niu, Jiayu, Yan, Ying, Fang, Yuan, Ke, Lihong, Chen, Wenju, Xu, Huijun, Li, Huimin, Hu, Xiaoxiu, Cao, Lulu, Chen, Yaolin, Tu, Hong, He, Yifu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113707/
https://www.ncbi.nlm.nih.gov/pubmed/35592651
http://dx.doi.org/10.3389/fcimb.2022.894836
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author Li, Mengge
Wu, Shusheng
Luo, Huiqin
Niu, Jiayu
Yan, Ying
Fang, Yuan
Ke, Lihong
Chen, Wenju
Xu, Huijun
Li, Huimin
Hu, Xiaoxiu
Cao, Lulu
Chen, Yaolin
Tu, Hong
He, Yifu
author_facet Li, Mengge
Wu, Shusheng
Luo, Huiqin
Niu, Jiayu
Yan, Ying
Fang, Yuan
Ke, Lihong
Chen, Wenju
Xu, Huijun
Li, Huimin
Hu, Xiaoxiu
Cao, Lulu
Chen, Yaolin
Tu, Hong
He, Yifu
author_sort Li, Mengge
collection PubMed
description Hepatitis B virus (HBV) infection has been reported to be associated with gastric cancer (GC). Nonetheless, no study has revealed the role of HBV infection in the survival of patients with GC, and the mutation profiles of HBV-infected patients with GC have never been documented. Here, we performed an updated meta-analysis and found a significantly increased risk of GC in HBV-infected individuals (sOR, 1.29; 95% CI, 1.22-1.37). Furthermore, we observed that in the Anhui area, the rate of serum HBsAg positivity (OR, 1.62; 95% CI, 1.03-2.55) was significantly higher in GC patients than in controls. Moreover, our results showed that HBV-positive patients had significantly worse disease-free survival (HR, 1.98; 95% CI, 1.39-2.82) and overall survival (HR, 1.84; 95% CI, 1.19-2.85) than HBV-negative patients. The results of Cox proportional hazards regression proved that HBV infection was an independent adverse prognostic factor in GC. Furthermore, by performing targeted-NGS, we found unique mutation profiles in HBV-infected GC samples, including five frequently mutated protein-coding genes (KMT2B, KMT2D, SOX1, FGF12, and TUBB2B). Expression and survival analyses of these genes identified three novel candidate genes that may have potential roles in GC development. Gene Ontology enrichment analysis showed that the recurrent mutations in HBV-positive GC samples were related to cell proliferation, cell migration, and transcription. Taking together, our study proved that HBV infection is an independent prognostic factor in GC patients. The unique mutation profiles of HBV-infected patients with GC open a new research direction toward the underling mechanism between HBV infection and GC.
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spelling pubmed-91137072022-05-18 Serological and Molecular Characterization of Hepatitis B Virus Infection in Gastric Cancer Li, Mengge Wu, Shusheng Luo, Huiqin Niu, Jiayu Yan, Ying Fang, Yuan Ke, Lihong Chen, Wenju Xu, Huijun Li, Huimin Hu, Xiaoxiu Cao, Lulu Chen, Yaolin Tu, Hong He, Yifu Front Cell Infect Microbiol Cellular and Infection Microbiology Hepatitis B virus (HBV) infection has been reported to be associated with gastric cancer (GC). Nonetheless, no study has revealed the role of HBV infection in the survival of patients with GC, and the mutation profiles of HBV-infected patients with GC have never been documented. Here, we performed an updated meta-analysis and found a significantly increased risk of GC in HBV-infected individuals (sOR, 1.29; 95% CI, 1.22-1.37). Furthermore, we observed that in the Anhui area, the rate of serum HBsAg positivity (OR, 1.62; 95% CI, 1.03-2.55) was significantly higher in GC patients than in controls. Moreover, our results showed that HBV-positive patients had significantly worse disease-free survival (HR, 1.98; 95% CI, 1.39-2.82) and overall survival (HR, 1.84; 95% CI, 1.19-2.85) than HBV-negative patients. The results of Cox proportional hazards regression proved that HBV infection was an independent adverse prognostic factor in GC. Furthermore, by performing targeted-NGS, we found unique mutation profiles in HBV-infected GC samples, including five frequently mutated protein-coding genes (KMT2B, KMT2D, SOX1, FGF12, and TUBB2B). Expression and survival analyses of these genes identified three novel candidate genes that may have potential roles in GC development. Gene Ontology enrichment analysis showed that the recurrent mutations in HBV-positive GC samples were related to cell proliferation, cell migration, and transcription. Taking together, our study proved that HBV infection is an independent prognostic factor in GC patients. The unique mutation profiles of HBV-infected patients with GC open a new research direction toward the underling mechanism between HBV infection and GC. Frontiers Media S.A. 2022-05-03 /pmc/articles/PMC9113707/ /pubmed/35592651 http://dx.doi.org/10.3389/fcimb.2022.894836 Text en Copyright © 2022 Li, Wu, Luo, Niu, Yan, Fang, Ke, Chen, Xu, Li, Hu, Cao, Chen, Tu and He https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Li, Mengge
Wu, Shusheng
Luo, Huiqin
Niu, Jiayu
Yan, Ying
Fang, Yuan
Ke, Lihong
Chen, Wenju
Xu, Huijun
Li, Huimin
Hu, Xiaoxiu
Cao, Lulu
Chen, Yaolin
Tu, Hong
He, Yifu
Serological and Molecular Characterization of Hepatitis B Virus Infection in Gastric Cancer
title Serological and Molecular Characterization of Hepatitis B Virus Infection in Gastric Cancer
title_full Serological and Molecular Characterization of Hepatitis B Virus Infection in Gastric Cancer
title_fullStr Serological and Molecular Characterization of Hepatitis B Virus Infection in Gastric Cancer
title_full_unstemmed Serological and Molecular Characterization of Hepatitis B Virus Infection in Gastric Cancer
title_short Serological and Molecular Characterization of Hepatitis B Virus Infection in Gastric Cancer
title_sort serological and molecular characterization of hepatitis b virus infection in gastric cancer
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113707/
https://www.ncbi.nlm.nih.gov/pubmed/35592651
http://dx.doi.org/10.3389/fcimb.2022.894836
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