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Synchronization in renal microcirculation unveiled with high-resolution blood flow imaging

Internephron interaction is fundamental for kidney function. Earlier studies have shown that nephrons signal to each other, synchronize over short distances, and potentially form large synchronized clusters. Such clusters would play an important role in renal autoregulation, but due to the technolog...

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Autores principales: Postnov, Dmitry, Marsh, Donald J, Cupples, Will A, Holstein-Rathlou, Niels-Henrik, Sosnovtseva, Olga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113743/
https://www.ncbi.nlm.nih.gov/pubmed/35522041
http://dx.doi.org/10.7554/eLife.75284
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author Postnov, Dmitry
Marsh, Donald J
Cupples, Will A
Holstein-Rathlou, Niels-Henrik
Sosnovtseva, Olga
author_facet Postnov, Dmitry
Marsh, Donald J
Cupples, Will A
Holstein-Rathlou, Niels-Henrik
Sosnovtseva, Olga
author_sort Postnov, Dmitry
collection PubMed
description Internephron interaction is fundamental for kidney function. Earlier studies have shown that nephrons signal to each other, synchronize over short distances, and potentially form large synchronized clusters. Such clusters would play an important role in renal autoregulation, but due to the technological limitations, their presence is yet to be confirmed. In the present study, we introduce an approach for high-resolution laser speckle imaging of renal blood flow and apply it to estimate the frequency and phase differences in rat kidney microcirculation under different conditions. The analysis unveiled the spatial and temporal evolution of synchronized blood flow clusters of various sizes, including the formation of large (>90 vessels) and long-lived clusters (>10 periods) locked at the frequency of the tubular glomerular feedback mechanism. Administration of vasoactive agents caused significant changes in the synchronization patterns and, thus, in nephrons’ co-operative dynamics. Specifically, infusion of vasoconstrictor angiotensin II promoted stronger synchronization, while acetylcholine caused complete desynchronization. The results confirm the presence of the local synchronization in the renal microcirculatory blood flow and that it changes depending on the condition of the vascular network and the blood pressure, which will have further implications for the role of such synchronization in pathologies development.
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spelling pubmed-91137432022-05-18 Synchronization in renal microcirculation unveiled with high-resolution blood flow imaging Postnov, Dmitry Marsh, Donald J Cupples, Will A Holstein-Rathlou, Niels-Henrik Sosnovtseva, Olga eLife Medicine Internephron interaction is fundamental for kidney function. Earlier studies have shown that nephrons signal to each other, synchronize over short distances, and potentially form large synchronized clusters. Such clusters would play an important role in renal autoregulation, but due to the technological limitations, their presence is yet to be confirmed. In the present study, we introduce an approach for high-resolution laser speckle imaging of renal blood flow and apply it to estimate the frequency and phase differences in rat kidney microcirculation under different conditions. The analysis unveiled the spatial and temporal evolution of synchronized blood flow clusters of various sizes, including the formation of large (>90 vessels) and long-lived clusters (>10 periods) locked at the frequency of the tubular glomerular feedback mechanism. Administration of vasoactive agents caused significant changes in the synchronization patterns and, thus, in nephrons’ co-operative dynamics. Specifically, infusion of vasoconstrictor angiotensin II promoted stronger synchronization, while acetylcholine caused complete desynchronization. The results confirm the presence of the local synchronization in the renal microcirculatory blood flow and that it changes depending on the condition of the vascular network and the blood pressure, which will have further implications for the role of such synchronization in pathologies development. eLife Sciences Publications, Ltd 2022-05-06 /pmc/articles/PMC9113743/ /pubmed/35522041 http://dx.doi.org/10.7554/eLife.75284 Text en © 2022, Postnov et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Medicine
Postnov, Dmitry
Marsh, Donald J
Cupples, Will A
Holstein-Rathlou, Niels-Henrik
Sosnovtseva, Olga
Synchronization in renal microcirculation unveiled with high-resolution blood flow imaging
title Synchronization in renal microcirculation unveiled with high-resolution blood flow imaging
title_full Synchronization in renal microcirculation unveiled with high-resolution blood flow imaging
title_fullStr Synchronization in renal microcirculation unveiled with high-resolution blood flow imaging
title_full_unstemmed Synchronization in renal microcirculation unveiled with high-resolution blood flow imaging
title_short Synchronization in renal microcirculation unveiled with high-resolution blood flow imaging
title_sort synchronization in renal microcirculation unveiled with high-resolution blood flow imaging
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113743/
https://www.ncbi.nlm.nih.gov/pubmed/35522041
http://dx.doi.org/10.7554/eLife.75284
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